Sustained release of stem cell secretome from nano-villi chitosan microspheres for effective treatment of atopic dermatitis.

Canine atopic dermatitis (AD) arises from hypersensitive immune reactions. AD symptoms entail severe pruritus and skin inflammation, with frequent relapses. Consequently, AD patients require continuous management, imposing financial burdens and mental fatigue on pet owners. In this study, we aimed to investigate the therapeutic relevance of secretome from canine adipose tissue-derived mesenchymal stem cells (MSCs), especially after encapsulation in nano-villi chitosan microspheres (CS-MS) to expect improved efficacy. Conditioned media (CM) from MSCs significantly inhibited the proliferation of splenocytes, induced the generation of regulatory T cells, and decreased mast cell degranulation. We found that beneficial soluble factors known to reduce AD symptoms, including transforming growth factor-beta 1, were detectable after sequential concentration and lyophilization of CM. The CS-MS, developed by a phase inversion regeneration method, showed high loading and sustained release of the secretome. Local injection of secretome-loaded CS-MS (ST/SC-MS) effectively reduced clinical severity compared to groups treated with secretome. Histological analysis revealed that ST/SC-MS potently suppressed epidermal hyperplasia, immunocyte infiltration and mast cell activation in the lesion. Taken together, this study presents a novel therapeutic approach exhibiting more potent and prolonged immunoregulatory efficacy of MSC secretome for canine AD treatment.

Low frequency changes in CO2 concentration in East Asia related to Pacific decadal oscillation and Atlantic multi-decadal oscillation for mid-summer and early fall.

The climatological seasonal maximum and minimum CO2 concentrations in East Asia for 1987-2020 have been recorded at April and August, respectively. We found that the CO2 concentration in East Asia during July, August, and September (JAS) is lower than normal before the late 1990s and after the early 2010s (Low_CO2 period), and higher than normal from the late 1990s to the early 2010s (High_CO2 period). The low-frequency variability of CO2 concentration in East Asia during JAS correlates with both Pacific Decadal Oscillation (PDO) and Atlantic Multi-decadal Oscillation (AMO)-related sea surface temperatures (SSTs). We analyzed atmospheric and oceanic conditions during JAS between the two periods, finding that precipitation in East Asia decreased during JAS in High_CO2 period than that in Low_CO2 period, possibly due to PDO and AMO-related SST forcing, which decreases vegetation's photosynthetic activity. This may lead to a higher CO2 concentration than normal in East Asia in High_CO2 period through reduced uptake of CO2 from the atmosphere. This implies that terrestrial vegetation activity influenced by remote SST forcings should be monitored to better understand regional carbon cycles in East Asia.

In-depth analysis on electrical parameters of floating gate IGZO synaptic transistor affecting pattern recognition accuracy.

The reliable conductance modulation of synaptic devices is key when implementing high-performance neuromorphic systems. Herein, we propose a floating gate indium gallium zinc oxide (IGZO) synaptic device with an aluminum trapping layer to investigate the correlation between its diverse electrical parameters and pattern recognition accuracy. Basic synaptic properties such as excitatory postsynaptic current, paired pulse facilitation, long/short term memory, and long-term potentiation/depression are demonstrated in the IGZO synaptic transistor. The effects of pulse tuning conditions associated with the pulse voltage magnitude, interval, duration, and cycling number of the applied pulses on the conductance update are systematically investigated. It is discovered that both the nonlinearity of the conductance update and cycle-to-cycle variation should be critically considered using an artificial neural network simulator to ensure the high pattern recognition accuracy of Modified National Institute of Standards and Technology (MNIST) handwritten digit images. The highest recognition rate of the MNIST handwritten dataset is 94.06% for the most optimized pulse condition. Finally, a systematic study regarding the synaptic parameters must be performed to optimize the developed synapse device.

In-depth analysis on electrical parameters of floating gate IGZO synaptic transistor affecting pattern recognition accuracy.

The reliable conductance modulation of synaptic devices is key when implementing high-performance neuromorphic systems. Herein, we propose a floating gate IGZO synaptic device with an aluminum trapping layer to investigate the correlation between its diverse electrical parameters and pattern recognition accuracy. Basic synaptic properties such as excitatory postsynaptic current, paired pulse facilitation, long/short term memory, and long-term potentiation/depression are demonstrated in the IGZO synaptic transistor. The effects of pulse tuning conditions associated with the pulse voltage magnitude, interval, duration, and cycling number of the applied pulses on the conductance update are systematically investigated. It is discovered that both the nonlinearity of the conductance update and cycle-to-cycle variation should be critically considered using an artificial neural network simulator to ensure the high pattern recognition accuracy of Modified National Institute of Standards and Technology (MNIST) handwritten digit images. The highest recognition rate of the MNIST handwritten dataset is 94.06% for the most optimized pulse condition. Finally, a systematic study regarding the synaptic parameters must be performed to optimize the developed synapse device.

Preclinical mouse model of optical coherence tomography for subcortical brain imaging without dissection.

The purpose of this study was to investigate the feasibility of using optical coherence tomography (OCT) to identify internal brain lesions, specifically intracerebral hemorrhage, without dissection. Mice with artificially injected brain hematomas were used to test the OCT system, and the recorded images were compared with microscopic images of the same mouse brains after hematoxylin and eosin staining. The intracranial structures surrounding the hematomas were clearly visualized by the OCT system without dissection. These images reflect the ability of OCT to determine the extent of a lesion in several planes. OCT is a useful technology, and these findings could be used as a starting point for future research in intraoperative imaging.

Characteristic distribution patterns of perfluoroalkyl substances in soils according to land-use types.

In this study, a nationwide monitoring of perfluoroalkyl substances (PFAS) in soils was conducted for various land-use types around South Korea, such as industrial complexes, landfills, farmlands, mountains, and woodlands. The ∑15PFAS concentrations in soil samples ranged from 0.175 to 11.7 ng/g dry weight (dw) (median 2.39 ng/g dw). The soil samples from industrial complexes (0.346-11.7 ng/g dw; median 3.25 ng/g dw) and landfills (0.504-10.4 ng/g dw; median 2.13 ng/g dw) had higher median ∑15PFAS concentrations than other regions (0.175-3.01 ng/g dw; median 1.15 ng/g dw). In the soil samples from industrial areas, the ∑15PFAS concentrations ranged from 3.11 to 11.7 ng/g dw (median 6.32 ng/g dw) from the textile industries, 3.26-8.74 ng/g dw (median 6.20 ng/g dw) from the metal industries, 1.53-5.71 ng/g dw (median 3.38 ng/g) from the chemical industries, and 0.346-3.48 ng/g dw (median 2.39 ng/g dw) from the electronics industries. Perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), and long chain perfluorinated carboxylic acids (PFCAs) between C9 and C12 were generally dominant in these soils. In particular, PFOS, perfluorohexane sulfonate (PFHxS), and PFCAs with ≤ C6 were more frequently found in soils from industrial complexes than from other land-use types. On the other hand, PFCAs between C7 and C12 had relatively high detection frequencies regardless of the soil type.

Evaluation of the current contamination status of PFASs and OPFRs in South Korean tap water associated with its origin.

We investigated the concentrations of perfluoroalkyl substances (PFASs) and organophosphate flame retardants (OPFRs) in 44 tap water samples, collected from eight major cities in South Korea served by four representative watersheds, to evaluate the water contamination status. The total concentrations of PFASs and OPFRs ranged from 1.44 to 224ng/L (median=11.9ng/L), and 74.0 to 342ng/L (median=151ng/L), respectively. The predominant compounds in tap water were perfluorohexane sulfonate (PFHxS), perfluoropentanoic acid (PFPeA), perfluorohexanoic acid (PFHxA), perfluorooctanoic acid (PFOA), tris(2-chloroethyl) phosphate (TCEP), tris(chloroisopropyl) phosphate (TCIPP), and tris(2-butoxyethyl) phosphate (TBOEP). Tap water originating from the Nakdong River within an industrial complex showed a notably higher PFHxS proportion to total PFASs. In addition, significantly higher PFAS levels were found in river-originating tap water than in lake/reservoir-originating tap water (Mann-Whitney U test, p<0.05). Meanwhile, major OPFRs showed no clear difference in distribution by region, and no significant difference in major OPFR levels was observed according to tap water origin. Finally, the average human exposure via tap water consumption was estimated for PFASs (46.8ng/person/day) and OPFRs (254ng/person/day).