An Energy-Efficient Multi-Level Sleep Strategy for Periodic Uplink Transmission in Industrial Private 5G Networks.

This paper proposes an energy-efficient multi-level sleep mode control for periodic transmission (MSC-PUT) in private fifth-generation (5G) networks. In general, private 5G networks meet IIoT requirements but face rising energy consumption due to dense base station (BS) deployment, particularly impacting operating expenses (OPEX). An approach of BS sleep mode has been studied to reduce energy consumption, but there has been insufficient consideration for the periodic uplink transmission of industrial Internet of Things (IIoT) devices. Additionally, 5G New Reno's synchronization signal interval limits the effectiveness of the deepest sleep mode in reducing BS energy consumption. By addressing this issue, the aim of this paper is to propose an energy-efficient multi-level sleep mode control for periodic uplink transmission to improve the energy efficiency of BSs. In advance, we develop an energy-efficient model that considers the trade-off between throughput impairment caused by increased latency and energy saving by sleep mode operation for IIoT's periodic uplink transmission. Then, we propose an approach based on proximal policy optimization (PPO) to determine the deep sleep mode of BSs, considering throughput impairment and energy efficiency. Our simulation results verify the proposed MSC-PUT algorithm's effectiveness in terms of throughput, energy saving, and energy efficiency. Specifically, we verify that our proposed MSC-PUT enhances energy efficiency by nearly 27.5% when compared to conventional multi-level sleep operation and consumes less energy at 75.21% of the energy consumed by the conventional method while incurring a throughput impairment of nearly 4.2%. Numerical results show that the proposed algorithm can significantly reduce the energy consumption of BSs accounting for periodic uplink transmission of IIoT devices.

CRISPRi-TnSeq: A genome-wide high-throughput tool for bacterial essential-nonessential genetic interaction mapping.

Genetic interaction networks can help identify functional connections between genes and pathways, which can be leveraged to establish (new) gene function, drug targets, and fill pathway gaps. Since there is no optimal tool that can map genetic interactions across many different bacterial strains and species, we develop CRISPRi-TnSeq, a genome-wide tool that maps genetic interactions between essential genes and nonessential genes through the knockdown of a targeted essential gene (CRISPRi) and the simultaneous knockout of individual nonessential genes (Tn-Seq). CRISPRi-TnSeq thereby identifies, on a genome-wide scale, synthetic and suppressor-type relationships between essential and nonessential genes, enabling the construction of essential-nonessential genetic interaction networks. To develop and optimize CRISPRi-TnSeq, CRISPRi strains were obtained for 13 essential genes in Streptococcus pneumoniae, involved in different biological processes including metabolism, DNA replication, transcription, cell division and cell envelope synthesis. Transposon-mutant libraries were constructed in each strain enabling screening of ∼24,000 gene-gene pairs, which led to the identification of 1,334 genetic interactions, including 754 negative and 580 positive genetic interactions. Through extensive network analyses and validation experiments we identify a set of 17 pleiotropic genes, of which a subset tentatively functions as genetic capacitors, dampening phenotypic outcomes and protecting against perturbations. Furthermore, we focus on the relationships between cell wall synthesis, integrity and cell division and highlight: 1) how essential gene knockdown can be compensated by rerouting flux through nonessential genes in a pathway; 2) the existence of a delicate balance between Z-ring formation and localization, and septal and peripheral peptidoglycan (PG) synthesis to successfully accomplish cell division; 3) the control of c-di-AMP over intracellular K + and turgor, and thereby modulation of the cell wall synthesis machinery; 4) the dynamic nature of cell wall protein CozEb and its effect on PG synthesis, cell shape morphology and envelope integrity; 5) functional dependency between chromosome decatenation and segregation, and the critical link with cell division, and cell wall synthesis. Overall, we show that CRISPRi-TnSeq uncovers genetic interactions between closely functionally linked genes and pathways, as well as disparate genes and pathways, highlighting pathway dependencies and valuable leads for gene function. Importantly, since both CRISPRi and Tn-Seq are widely used tools, CRISPRi-TnSeq should be relatively easy to implement to construct genetic interaction networks across many different microbial strains and species.