[Spectrofluorometric analysis of protein fractions of blood serum of health donors and patients with kidney diseases]. / Spektrofluorimetricheskii analiz belkovykh fraktsii syvorotki krovi zdorovykh donorov i bol'nykh s zabolevaniiami pochek.

A comparison was made of ultra-violet fluorescence characteristics of albumin enriched protein fractions of serum blood of 35 glomerulonephritis (GN) patients, 30 uremic haemodialysis patients, and 40 donors. It has been found that fluorescence spectra of the albumin enriched fractions of serum from GN patients are blue shifted as compared with those of donors' serum preparations. At the same time fluorescence spectra of the albumin enriched fractions of donors' serum are similar, while those of GN patients vary significantly. The ratio of fluorescence intensities at 320 and 365 nm (A = I320/I365), characterizing the spectrum position is 1.27 +/- 0.05, for protein preparations of donors, while that for GN patients varies within the limits of 1.3-2.1. Fluorescence spectra of protein fractions from blood serum of uremic patients are red shifted (A = 0.77-1.29) in comparison with those of donors' blood. Chromatographic investigations show that protein preparations of GN patients' blood contain monomers and dimers of albumin, that can be divided according to their molecular masses or hydrophobic properties of the surface. Joint chromatographic and spectral analysis allowed to distinguish up to six albumin enriched fractions. A protein fraction with blue fluorescence spectrum was obtained by gel-filtration and ion exchange chromatography. A higher concentration of this fraction determines a high value of parameter A, that is typical for protein preparations of GN patients' blood. Amino acid sequence shows that one component of this fraction is beta-haptoglobin.

[The detection of modified forms of serum albumin in glomerulonephritis patients by spectrofluorometry]. / Obnaruzhenie modifitsirovannykh form syvorotochnogo al'bumina u bol'nykh glomerulonefritom s pomoshch'iu spektrofliuorimetrii.

A comparative investigation covered UV-fluorescence characteristics of partially purified preparations of serum albumin (SA) in 16 glomerulonephritis (GN) patients and 12 healthy subjects. Fluorescence spectra of SA in GN patients proved shifted to short-wave region, this evidencing differences in SA structure of the patients and healthy subjects. The fluorescence spectra were characterized by parameter A. A = I320/I365, where I320 and I365 are intensities at the spectra in the wave lengths 320 and 365 nm, respectively. In 16 GN patients with normal renal function an inverse relationship was derived between the A value and blood albumin levels. A value and daily protein loss were related directly. This may denote predominant passage of structurally deformed albumin through glomerular filter.

[Effect of heavy water on the viability of bacteria]. / Vliianie tiazheloi vody na protsessy zhiznedeiatel'nosti bakterii.

Influence of heavy water (D2O) on the membrane energization, the efflux of hydrogen ions and the respiration of bacteria E. coli M-17 was studied. As has been shown, heavy water of a low concentration (0.05-0.20% v/v) activates and of a high concentration (above 10%) inhibits the absorption of lipophilic cation tetraphenylphosphonium (TPP+) and of oxygen by cells. The return of these characteristics to the initial levels after the removal of D2O points to a reversible action of D2O. A protective effect of D2O towards membrane energization and rate of respiration on dried cells was observed. This fact is in agreement with the data on viability of bacteria. The indicated protective action increases at the stage of rehydration in the presence of D2O.