Meta-omic evaluation of bacterial microbial community structure and activity for the environmental assessment of soils: overcoming protein extraction pitfalls.

Microorganisms play unique, essential and integral roles in the biosphere. This work aims to assess the utility of soil's metaomics for environmental diagnosis. Doñana National Park (DNP) was selected as a natural lab since it contains a strictly protected core that is surrounded by numerous threats of pollution. Culture-independent high-throughput molecular tools were used to evaluate the alterations of the global structure and metabolic activities of the microbiome. 16S rRNA sequencing shows lower bacterial abundance and diversity in areas historically exposed to contamination that surround DNP. For metaproteomics, an innovative post-alkaline protein extraction protocol was developed. After NaOH treatment, successive washing with Tris-HCl buffer supplemented with glycerol was essential to eliminate interferences. Starting from soils with different physicochemical characteristics, the method renders proteins with a remarkable resolution on SDS-PAGE gels. The proteins extracted were analysed by using an in-house database constructed from the rRNA data. LC-MS/MS analysis identified 2182 non-redundant proteins with 135 showing significant differences in relative abundance in the soils around DNP. Relevant global biological processes were altered in response to the environmental changes, such as protective and antioxidant mechanisms, translation, folding and homeostasis of proteins, membrane transport and aerobic respiratory metabolism.

Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden-Meyerhof-Parnas and Entner-Doudoroff pathways.

BACKGROUND: The halophilic bacterium Chromohalobacter salexigens metabolizes glucose exclusively through the Entner-Doudoroff (ED) pathway, an adaptation which results in inefficient growth, with significant carbon overflow, especially at low salinity. Preliminary analysis of C. salexigens genome suggests that fructose metabolism could proceed through the Entner-Doudoroff and Embden-Meyerhof-Parnas (EMP) pathways. In order to thrive at high salinity, this bacterium relies on the biosynthesis and accumulation of ectoines as major compatible solutes. This metabolic pathway imposes a high metabolic burden due to the consumption of a relevant proportion of cellular resources, including both energy molecules (NADPH and ATP) and carbon building blocks. Therefore, the existence of more than one glycolytic pathway with different stoichiometries may be an advantage for C. salexigens. The aim of this work is to experimentally characterize the metabolism of fructose in C. salexigens. RESULTS: Fructose metabolism was analyzed using in silico genome analysis, RT-PCR, isotopic labeling, and genetic approaches. During growth on fructose as the sole carbon source, carbon overflow was not observed in a wide range of salt concentrations, and higher biomass yields were reached. We unveiled the initial steps of the two pathways for fructose incorporation and their links to central metabolism. While glucose is metabolized exclusively through the Entner-Doudoroff (ED) pathway, fructose is also partially metabolized by the Embden-Meyerhof-Parnas (EMP) route. Tracking isotopic label from [1-13C] fructose to ectoines revealed that 81% and 19% of the fructose were metabolized through ED and EMP-like routes, respectively. Activities of enzymes from both routes were demonstrated in vitro by 31P-NMR. Genes encoding predicted fructokinase and 1-phosphofructokinase were cloned and the activities of their protein products were confirmed. Importantly, the protein encoded by csal1534 gene functions as fructose bisphosphatase, although it had been annotated previously as pyrophosphate-dependent phosphofructokinase. The gluconeogenic rather than glycolytic role of this enzyme in vivo is in agreement with the lack of 6-phosphofructokinase activity previously described. CONCLUSIONS: Overall, this study shows that C. salexigens possesses a greater metabolic flexibility for fructose catabolism, the ED and EMP pathways contributing to a fine balancing of energy and biosynthetic demands and, subsequently, to a more efficient metabolism.

Perforación esofágica durante el monitoreo intraoperatorio con ecocardiografía transesofágica. Reparación en el mismo acto quirúrgico / Esophageal injury secondary to transesophageal ecocardiography monitoring and repair

Monitoring with intraoperative Transesophageal Echocardiography (TEE) has proven to be a tool of very high utility for both control of the hemodynamic status, cardiac function and to make new diagnoses. It is a minimally invasive technique and, like any medical procedure, it is not exempt from complications that do not exceed 1%. These range from mild oropharyngeal lesions to the most serious lesion, esophageal perforation. We describe a case of esophageal perforation from the esophagogastric junction to the middle third of the esophagus in the intraoperative period of laparoscopic Nissen surgery. The injury was repaired immediately and the closure of the lesion was verified with pneumatic maneuvers through the nasogastric tube. The patient was discharged after 35 days

El monitoreo con Ecocardiografía Transesofágica intraoperatorio (ETE) ha demostrado ser una herramienta de muy alta utilidad tanto para control del estado hemodinámico, función cardíaca y para realizar nuevos diagnósticos. Se trata de una técica seiinvasiva y como todo procedimiento médico no está exento de complicaciones que no superan al 1%. Estas son desde lesiones leves orofaríngeas hasta la lesión más grave que es la perforación esofágica. Se describe un caso de perforación esofágica con desgrarro de éste desde la unión esofagogástrica hacia el tercio medio del esófago en el íntraoperatorio de cirugía de Nissen laparoscópico. La reparación de la injuria se realizó en forma inmediata y se comprobó con maniobras neumáticas a través de la sonda nasogástrica el cierre de la lesión. La paciente fue dada de alta a los 35 días.

Insights into metabolic osmoadaptation of the ectoines-producer bacterium Chromohalobacter salexigens through a high-quality genome scale metabolic model.

BACKGROUND: The halophilic bacterium Chromohalobacter salexigens is a natural producer of ectoines, compatible solutes with current and potential biotechnological applications. As production of ectoines is an osmoregulated process that draws away TCA intermediates, bacterial metabolism needs to be adapted to cope with salinity changes. To explore and use C. salexigens as cell factory for ectoine(s) production, a comprehensive knowledge at the systems level of its metabolism is essential. For this purpose, the construction of a robust and high-quality genome-based metabolic model of C. salexigens was approached. RESULTS: We generated and validated a high quality genome-based C. salexigens metabolic model (iFP764). This comprised an exhaustive reconstruction process based on experimental information, analysis of genome sequence, manual re-annotation of metabolic genes, and in-depth refinement. The model included three compartments (periplasmic, cytoplasmic and external medium), and two salinity-specific biomass compositions, partially based on experimental results from C. salexigens. Using previous metabolic data as constraints, the metabolic model allowed us to simulate and analyse the metabolic osmoadaptation of C. salexigens under conditions for low and high production of ectoines. The iFP764 model was able to reproduce the major metabolic features of C. salexigens. Flux Balance Analysis (FBA) and Monte Carlo Random sampling analysis showed salinity-specific essential metabolic genes and different distribution of fluxes and variation in the patterns of correlation of reaction sets belonging to central C and N metabolism, in response to salinity. Some of them were related to bioenergetics or production of reducing equivalents, and probably related to demand for ectoines. Ectoines metabolic reactions were distributed according to its correlation in four modules. Interestingly, the four modules were independent both at low and high salinity conditions, as they did not correlate to each other, and they were not correlated with other subsystems. CONCLUSIONS: Our validated model is one of the most complete curated networks of halophilic bacteria. It is a powerful tool to simulate and explore C. salexigens metabolism at low and high salinity conditions, driving to low and high production of ectoines. In addition, it can be useful to optimize the metabolism of other halophilic bacteria for metabolite production.

Understanding the interplay of carbon and nitrogen supply for ectoines production and metabolic overflow in high density cultures of Chromohalobacter salexigens.

BACKGROUND: The halophilic bacterium Chromohalobacter salexigens has been proposed as promising cell factory for the production of the compatible solutes ectoine and hydroxyectoine. This bacterium has evolved metabolic adaptations to efficiently grow under high salt concentrations by accumulating ectoines as compatible solutes. However, metabolic overflow, which is a major drawback for the efficient conversion of biological feedstocks, occurs as a result of metabolic unbalances during growth and ectoines production. Optimal production of ectoines is conditioned by the interplay of carbon and nitrogen metabolisms. In this work, we set out to determine how nitrogen supply affects the production of ectoines. RESULTS: Chromohalobacter salexigens was challenged to grow in media with unbalanced carbon/nitrogen ratio. In C. salexigens, overflow metabolism and ectoines production are a function of medium composition. At low ammonium conditions, the growth rate decreased importantly, up to 80%. Shifts in overflow metabolism were observed when changing the C/N ratio in the culture medium. 13C-NMR analysis of ectoines labelling revealed a high metabolic rigidity, with almost constant flux ratios in all conditions assayed. Unbalanced C/N ratio led to pyruvate accumulation, especially upon N-limitation. Analysis of an ect - mutant demonstrated the link between metabolic overflow and ectoine biosynthesis. Under non ectoine synthesizing conditions, glucose uptake and metabolic overflow decreased importantly. Finally, in fed-batch cultures, biomass yield was affected by the feeding scheme chosen. High growth (up to 42.4 g L-1) and volumetric ectoine yields (up to 4.21 g L-1) were obtained by minimizing metabolite overflow and nutrient accumulation in high density cultures in a low nitrogen fed-batch culture. Moreover, the yield coefficient calculated for the transformation of glucose into biomass was 30% higher in fed-batch than in the batch culture, demonstrating that the metabolic efficiency of C. salexigens can be improved by careful design of culture feeding schemes. CONCLUSIONS: Metabolic shifts observed at low ammonium concentrations were explained by a shift in the energy required for nitrogen assimilation. Carbon-limited fed-batch cultures with reduced ammonium supply were the best conditions for cultivation of C. salexigens, supporting high density growth and maintaining high ectoines production.

Effect of obstacle position in the flow of sheep through a narrow door.

In a recent work [Phys. Rev. E 91, 022808 (2015)PLEEE81539-375510.1103/PhysRevE.91.022808] it was reported that placing an obstacle in front of a gate has a beneficial effect in the flow of sheep through it. Here, we extend such results by implementing three different obstacle positions. We have observed that the flow is improved in two cases, while it worsens in the other one; the last instance happens when the obstacle is too close to the door. In this situation, the outcomes suggest that clogging develops between the doorjamb and the obstacle, contrary to the cases when the obstacle is farther, in which case clogging always occurs at the very door. The effectiveness of the obstacle (a strategy put forward to alleviate clogging in emergency exits) is therefore quite sensitive to its location. In addition, the study of the temporal evolution of the flow rate as the test develops makes evident a steady behavior during the entire duration of the entrance. This result is at odds with recent findings in human evacuation tests where the flow rate varies over time, therefore challenging the fairness of straightforward comparisons between pedestrian behavior and animal experimental observations.

Contribution of RpoS to metabolic efficiency and ectoines synthesis during the osmo- and heat-stress response in the halophilic bacterium Chromohalobacter salexigens.

Chromohalobacter salexigens is a halophilic γ-proteobacterium that responds to osmotic and heat stresses by accumulating ectoine and hydroxyectoine respectively. Evolution has optimized its metabolism to support high production of ectoines. We analysed the effect of an rpoS mutation in C. salexigens metabolism and ectoines synthesis. In long-term adapted cells, the rpoS strain was osmosensitive but not thermosensitive and showed unaltered ectoines content, suggesting that RpoS regulates ectoine(s)-independent osmoadaptive mechanisms. RpoS is involved in the regulation of C. salexigens metabolic adaptation to stress, as early steps of glucose oxidation through the Entner-Doudoroff pathway were deregulated in the rpoS mutant, leading to improved metabolic efficiency at low salinity. Moreover, a reduced pyruvate (but not acetate) overflow was displayed by the rpoS strain at low salt, probably linked to a slowdown in gluconate production and/or subsequent metabolism. Interestingly, RpoS does not seem to be the main regulator triggering the immediate transcriptional response of ectoine synthesis to osmotic or thermal upshifts. However, it contributed to the expression of the ect genes in cells previously adapted to low or high salinity.

Experimental proof of faster-is-slower in systems of frictional particles flowing through constrictions.

The "faster-is-slower" (FIS) effect was first predicted by computer simulations of the egress of pedestrians through a narrow exit [D. Helbing, I. J. Farkas, and T. Vicsek, Nature (London) 407, 487 (2000)]. FIS refers to the finding that, under certain conditions, an excess of the individuals' vigor in the attempt to exit causes a decrease in the flow rate. In general, this effect is identified by the appearance of a minimum when plotting the total evacuation time of a crowd as a function of the pedestrian desired velocity. Here, we experimentally show that the FIS effect indeed occurs in three different systems of discrete particles flowing through a constriction: (a) humans evacuating a room, (b) a herd of sheep entering a barn, and (c) grains flowing out a 2D hopper over a vibrated incline. This finding suggests that FIS is a universal phenomenon for active matter passing through a narrowing.

Role of central metabolism in the osmoadaptation of the halophilic bacterium Chromohalobacter salexigens.

Bacterial osmoadaptation involves the cytoplasmic accumulation of compatible solutes to counteract extracellular osmolarity. The halophilic and highly halotolerant bacterium Chromohalobacter salexigens is able to grow up to 3 m NaCl in a minimal medium due to the de novo synthesis of ectoines. This is an osmoregulated pathway that burdens central metabolic routes by quantitatively drawing off TCA cycle intermediaries. Consequently, metabolism in C. salexigens has adapted to support this biosynthetic route. Metabolism of C. salexigens is more efficient at high salinity than at low salinity, as reflected by lower glucose consumption, lower metabolite overflow, and higher biomass yield. At low salinity, by-products (mainly gluconate, pyruvate, and acetate) accumulate extracellularly. Using [1-(13)C]-, [2-(13)C]-, [6-(13)C]-, and [U-(13)C6]glucose as carbon sources, we were able to determine the main central metabolic pathways involved in ectoines biosynthesis from glucose. C. salexigens uses the Entner-Doudoroff pathway rather than the standard glycolytic pathway for glucose catabolism, and anaplerotic activity is high to replenish the TCA cycle with the intermediaries withdrawn for ectoines biosynthesis. Metabolic flux ratios at low and high salinity were similar, revealing a certain metabolic rigidity, probably due to its specialization to support high biosynthetic fluxes and partially explaining why metabolic yields are so highly affected by salinity. This work represents an important contribution to the elucidation of specific metabolic adaptations in compatible solute-accumulating halophilic bacteria.

Acetate scavenging activity in Escherichia coli: interplay of acetyl-CoA synthetase and the PEP-glyoxylate cycle in chemostat cultures.

Impairment of acetate production in Escherichia coli is crucial for the performance of many biotechnological processes. Aerobic production of acetate (or acetate overflow) results from changes in the expression of central metabolism genes. Acetyl-CoA synthetase scavenges extracellular acetate in glucose-limited cultures. Once converted to acetyl-CoA, it can be catabolized by the tricarboxylic acid cycle or the glyoxylate pathway. In this work, we assessed the significance of these pathways on acetate overflow during glucose excess and limitation. Gene expression, enzyme activities, and metabolic fluxes were studied in E. coli knock-out mutants related to the glyoxylate pathway operon and its regulators. The relevance of post-translational regulation by AceK-mediated phosphorylation of isocitrate dehydrogenase for pathway functionality was underlined. In chemostat cultures performed at increasing dilution rates, acetate overflow occurs when growing over a threshold glucose uptake rate. This threshold was not affected in a glyoxylate-pathway-deficient strain (lacking isocitrate lyase, the first enzyme of the pathway), indicating that it is not relevant for acetate overflow. In carbon-limited chemostat cultures, gluconeogenesis (maeB, sfcA, and pck), the glyoxylate operon and, especially, acetyl-CoA synthetase are upregulated. A mutant in acs (encoding acetyl-CoA synthetase) produced acetate at all dilution rates. This work demonstrates that, in E. coli, acetate production occurs at all dilution rates and that overflow is the result of unbalanced synthesis and scavenging activities. The over-expression of acetyl-CoA synthetase by cAMP-CRP-dependent induction limits this phenomenon in cultures consuming glucose at low rate, ensuring the recycling of the acetyl-CoA and acetyl-phosphate pools, although establishing an energy-dissipating substrate cycle.

Ectoines in cell stress protection: uses and biotechnological production.

Microorganisms produce and accumulate compatible solutes aiming at protecting themselves from environmental stresses. Among them, the wide spread in nature ectoines are receiving increasing attention by the scientific community because of their multiple applications. In fact, increasing commercial demand has led to a multiplication of efforts in order to improve processes for their production. In this review, the importance of current and potential applications of ectoines as protecting agents for macromolecules, cells and tissues, together with their potential as therapeutic agents for certain diseases are analyzed and current theories for the understanding of the molecular basis of their biological activity are discussed. The genetic, biochemical and environmental determinants of ectoines biosynthesis by natural and engineered producers are described. The major limitations of current bioprocesses used for ectoines production are discussed, with emphasis on the different microorganisms, environments, molecular engineering and fermentation strategies used to optimize the production and recovery of ectoines. The combined application of both bioprocess and metabolic engineering strategies, allowing a deeper understanding of the main factors controlling the production process is also stated. Finally, this review aims to summarize and update the state of the art in ectoines uses and applications and industrial scale production using bacteria, emphasizing the importance of reactor design and operation strategies, together with the metabolic engineering aspects and the need for feedback between wet and in silico work to optimize bioproduction.

“Consultas de pasillo” (curbside consultation): otra actividad asistencial del infectólogo / Curbside consultation: Another healthcare activity of the infectious disease specialist

Introducción Se define “consulta de pasillo” (CP) como el proceso informal en el que un médico obtiene información de otro médico en el tratamiento de un paciente que nunca ha valorado el médico consultado. Material y métodos Estudio prospectivo observacional que tiene como objetivo conocer la frecuencia, los contenidos, los servicios demandantes y el resultado final de las CP realizadas a la Unidad de Enfermedades Infecciosas (UEI) en el período del 25-03-2008 al 5-12-2008. Las variables recogidas eran relativas al medio de contacto, tipo de pregunta, persona solicitante, complejidad del tema, tema relativo, recomendaciones y grado de seguimiento y evolución de la consulta. El grado de dificultad se evaluó mediante el sistema de la ACP (American Collage of Physicians). Se emplearon métodos estadísticos paramétricos y no paramétricos. ResultadosSe recabaron 208 CP (1,13/día), con una mediana de 2min. Los motivos de consulta más frecuentes fueron seleccionar un antimicrobiano (54,4%), evaluar el diagnóstico (10,5%), interpretación de datos microbiológicos (9,6%) y la combinación de varios motivos (27,4%). En el 5,8% se precisó ingreso para estudio y tratamiento de la enfermedad infecciosa. Se requirió una interconsulta formal en el 27% de los casos. Se encontraron diferencias significativas entre médicos adjuntos y residentes y las realizadas por servicios médicos y quirúrgicos. Conclusiones Queremos destacar que las CP suponen una parte importante de la actividad asistencial de la UEI, aunque en su mayoría son sencillas y no suponen una carga de trabajo excesiva. Sin embargo, cuando se trata de consultas de caso clínico, realizadas por staff, con un grado de dificultad alto y que implican servicios quirúrgicos generan una actividad formal significativa (AU)

Introduction “Curbside consultation” is the term used to describe an informal process in which a physician requests information from another physician about the management of a patient who has not been assessed by the person consulted. Material and Methods Prospective, observational study designed to determine the frequency, services requested, and final result of curbside consultations made over the period of 3 March to 12 May 2008. The variables recorded included the means by which contact was made, the type of question, person requesting information, complexity of the subject, related subject, recommendations, and degree of follow-up and evolution of the consultation. The degree of difficulty was evaluated using the system of the American Collage of Physicians. Parametric and nonparametric statistical tests were used in the analysis. Results A total of 208 consultations (1.13/day) were reported, lasting a median of 2 minutes. The most common reasons for consulting were selection of an antimicrobial agent (54.4%), evaluation of a diagnosis (10.5%), interpretation of microbiological data (9.6%), and a combination of reasons (27.4%). In 5.8% of cases, hospitalization was required to study and treat the infectious disease. A formal consultation was required in 27% of cases. Significant differences were found between staff physicians and medical residents and between medical and surgical departments. Conclusions Curbside consultations comprise an important part of healthcare activity in the Infectious Disease Department, although most requests are easily resolved and do not imply an excessive work burden. Nonetheless, when the consultation involves a difficult clinical case, the request is by a staff physician, and the surgery department is implicated, a significant amount of formal activity is generated (AU)

[Curbside consultation: another healthcare activity of the infectious disease specialist]. / "Consultas de pasillo" (curbside consultation): otra actividad asistencial del infectólogo.

INTRODUCTION: "Curbside consultation" is the term used to describe an informal process in which a physician requests information from another physician about the management of a patient who has not been assessed by the person consulted. MATERIAL AND METHODS: Prospective, observational study designed to determine the frequency, services requested, and final result of curbside consultations made over the period of 3 March to 12 May 2008. The variables recorded included the means by which contact was made, the type of question, person requesting information, complexity of the subject, related subject, recommendations, and degree of follow-up and evolution of the consultation. The degree of difficulty was evaluated using the system of the American Collage of Physicians. Parametric and nonparametric statistical tests were used in the analysis. RESULTS: A total of 208 consultations (1.13/day) were reported, lasting a median of 2 minutes. The most common reasons for consulting were selection of an antimicrobial agent (54.4%), evaluation of a diagnosis (10.5%), interpretation of microbiological data (9.6%), and a combination of reasons (27.4%). In 5.8% of cases, hospitalization was required to study and treat the infectious disease. A formal consultation was required in 27% of cases. Significant differences were found between staff physicians and medical residents and between medical and surgical departments. CONCLUSIONS: Curbside consultations comprise an important part of healthcare activity in the Infectious Disease Department, although most requests are easily resolved and do not imply an excessive work burden. Nonetheless, when the consultation involves a difficult clinical case, the request is by a staff physician, and the surgery department is implicated, a significant amount of formal activity is generated.

An insight into the role of phosphotransacetylase (pta) and the acetate/acetyl-CoA node in Escherichia coli.

BACKGROUND: Acetate metabolism in Escherichia coli plays an important role in the control of the central metabolism and in bioprocess performance. The main problems related to the use of E. coli as cellular factory are i) the deficient utilization of carbon source due to the excretion of acetate during aerobic growth, ii) the inhibition of cellular growth and protein production by acetate and iii) the need for cofactor recycling (namely redox coenzymes and free CoASH) to sustain balanced growth and cellular homeostasis. RESULTS: This work analyzes the effect of mutations in the acetate excretion/assimilation pathways, acetyl-CoA synthethase (acs) and phosphotransacetylase (pta), in E. coli BW25113 grown on glucose or acetate minimal media. Biomass and metabolite production, redox (NADH/NAD+) and energy (ATP) state, enzyme activities and gene expression profiles related to the central metabolism were analyzed. The knock-out of pta led to a more altered phenotype than that of acs. Deletion of pta reduced the ability to grow on acetate as carbon source and strongly affected the expression of several genes related to central metabolic pathways. CONCLUSION: Results showed that pta limits biomass yield in aerobic glucose cultures, due to acetate production (overflow metabolism) and its inefficient use during glucose starvation. Deletion of pta severely impaired growth on acetate minimal medium and under anaerobiosis due to decreased acetyl-coenzyme A synthethase, glyoxylate shunt and gluconeogenic activities, leading to lower growth rate. When acetate is used as carbon source, the joint expression of pta and acs is crucial for growth and substrate assimilation, while pta deletion severely impaired anaerobic growth. Finally, at an adaptive level, pta deficiency makes the strain more sensitive to environmental changes and de-regulates the central metabolism.

Depth profiling by confocal Raman microspectroscopy: semi-empirical modeling of the Raman response.

It has been well documented that the use of dry optics in depth profiling by confocal Raman microspectroscopy significantly distorts the laser focal volume, thus negatively affecting the spatial resolution of the measurements. In that case, the resulting in-depth confocal profile is an outcome of several contributions: the broadening of the laser spot due to instrumental factors and diffraction, the spreading of the illuminated region due to refraction of the laser beam at the sample surface, and the influence of the confocal aperture in the collection path of the laser beam. Everall and Batchelder et al. developed simple models that describe the effect of the last two factors, i.e., laser refraction and the diameter of the pinhole aperture, on the confocal profile. In this work, we compare these theoretical predictions with experimental data obtained on a series of well-defined planar interfaces, generated by contact between thin polyethylene (PE) films (35, 53, 75, and 105 microm thickness) and a much thicker poly(methyl methacrylate) (PMMA) piece. We included two refinements in the above-mentioned models: the broadening of the laser spot due to instrumental factors and diffraction and a correction for the overestimation in the decay rate of collection efficiency predicted by Batchelder et al. These refinements were included through a semiempirical approach, consisting of independently measuring the Raman step-response in the absence of refraction by using a silicon wafer and the actual intensity decay of a thick and transparent polymer film. With these improvements, the model reliably reproduces fine features of the confocal profiles for both PE films and PMMA substrates. The results of this work show that these simple models can not only be used to assist data interpretation, but can also be used to quantitatively predict in-depth confocal profiles in experiments carried out with dry optics.

Infección en quemaduras graves. Nuevas perspectivas de tratamiento en atención primaria / No disponible

No disponible

Interphase evolution in polymer films by confocal Raman microspectroscopy.

Liquid-glassy polymer diffusion is an important topic in polymer physics, with several mechanistic aspects that still remain unclear. Here we describe the use of confocal Raman microspectroscopy (CRM) to study directly several features of interphase evolution in a system of this type. The interphase studied was generated by contact between liquid polystyrene (PS) and glassy polyphenylene oxide (PPO). Interphase evolution on thin films made from these polymers was followed by depth profiling in combination with immersion optics. We also applied regularized deconvolution to improve the spatial resolution of the measurements. With the help of these techniques, we examined interphase PPO concentration profiles and kinetics of interphase evolution in the range 120-180 degrees C, well below the glass transition temperature of the PPO-based films (185 degrees C). Overall, the experiment captures the most important features needed to discern the mechanistic factors that control this process. In this sense, confocal Raman microspectroscopy emerges as one of the best experimental techniques for the study of diffusion kinetics in this type of system.

Reforma de la unidad de hospitalización pediátrica del Hospital de Móstoles / Refurbishment in the paediatric hospitalization unit of the Hospital de Móstoles

Descripción del Plan Funcional de reforma de la Unidad de Hospitalización Pediátrica del Hospital de Móstoles (AU)

Description of the refurbishment Functional Plan of the Paediatric Hospitalization Unit of the Hospital de Móstoles (AU)

Eficacia, e influencia sobre la función ventilatoria de la analgesia regional intrapleural / Efficacy of regional interpleural analgesia and its influence on ventilatory function

Se estudiaron en el postoperatorio inmediato 20 pacientes A.S.A. I-II de cirugía electiva de tórax, abdomen superior, mama y cirugía renal, con el propósito de investigar un nuevo método de analgesia regional: la Analgesia Regional Intrapleural. Una vez finalizada la cirugía pero con el paciente aún dormido, se colocó en el octavo espacio intercostal por vía posterior un catéter epidural dentro del espacio intrapleural. Se describe la técnica de acceso al espacio pleural, y se discute el mecanismo de acción. Cuando los pacientes manifestaron dolor, se inyectaron 20 ml. de bupivacaína al 0,5 por ciento con epinefrina por el catéter intrapleural. Se evaluó la analgesia, su duración y la función ventilatoria mediante gasometría arterial y espirometría dinámica. Se consiguió analgesia completa de larga duración a los pocos minutos de inyectada la droga sin efectos secundarios significativos.

Eficacia, e influencia sobre la función ventilatoria de la analgesia regional intrapleural / Efficacy of regional interpleural analgesia and its influence on ventilatory function

Se estudiaron en el postoperatorio inmediato 20 pacientes A.S.A. I-II de cirugía electiva de tórax, abdomen superior, mama y cirugía renal, con el propósito de investigar un nuevo método de analgesia regional: la Analgesia Regional Intrapleural. Una vez finalizada la cirugía pero con el paciente aún dormido, se colocó en el octavo espacio intercostal por vía posterior un catéter epidural dentro del espacio intrapleural. Se describe la técnica de acceso al espacio pleural, y se discute el mecanismo de acción. Cuando los pacientes manifestaron dolor, se inyectaron 20 ml. de bupivacaína al 0,5 por ciento con epinefrina por el catéter intrapleural. Se evaluó la analgesia, su duración y la función ventilatoria mediante gasometría arterial y espirometría dinámica. Se consiguió analgesia completa de larga duración a los pocos minutos de inyectada la droga sin efectos secundarios significativos. (AU)