Nanocellulose-assisted mechanically tough hydrogel platforms for sustained drug delivery.

The controlled delivery of the desired bioactive molecules is required to achieve the maximum therapeutic effects with minimum side effects. Biopolymer-based hydrogels are ideal platforms for delivering the desired molecules owing to their superior biocompatibility, biodegradability, and low-immune response. However, the prolonged delivery of the drugs through biopolymer-based hydrogels is restricted due to their weak mechanical stability. We developed mechanically tough and biocompatible hydrogels to address these limitations using carboxymethyl chitosan, sodium alginate, and nanocellulose for sustained drug delivery. The hydrogels were cross-linked through calcium ions to enhance their mechanical strength. Nanocellulose-added hydrogels exhibited improved mechanical strength (Young's modulus; 23.36 → 30.7 kPa, Toughness; 1.39 → 5.65 MJm-3) than pure hydrogels. The composite hydrogels demonstrated increased recovery potential (66.9 → 84.5 %) due to the rapid reformation of damaged polymeric networks. The hydrogels were stable in an aqueous medium and demonstrated reduced swelling potential. The hydrogels have no adverse effects on embryonic murine fibroblast (3 T3), showing their biocompatibility. No bacterial growth was observed in hydrogels-treated groups, indicating their antibacterial characteristics. The sustained drug released was observed from nanocellulose-assisted hydrogel scaffolds compared to the pure polymer hydrogel scaffold. Thus, hydrogels have potential and could be used as a sustained drug carrier.

Intrinsically Multistable Soft Actuator Driven by Mixed-Mode Snap-Through Instabilities.

Actuators utilizing snap-through instabilities are widely investigated for high-performance fast actuators and shape reconfigurable structures owing to their rapid response and limited reliance on continuous energy input. However, prevailing approaches typically involve a combination of multiple bistable actuator units and achieving multistability within a single actuator unit still remains an open challenge. Here, a soft actuator is presented that uses shape memory alloy (SMA) and mixed-mode elastic instabilities to achieve intrinsically multistable shape reconfiguration. The multistable actuator unit consists of six stable states, including two pure bending states and four bend-twist states. The actuator is composed of a pre-stretched elastic membrane placed between two elastomeric frames embedded with SMA coils. By controlling the sequence and duration of SMA activation, the actuator is capable of rapid transition between all six stable states within hundreds of milliseconds. Principles of energy minimization are used to identify actuation sequences for various types of stable state transitions. Bending and twisting angles corresponding to various prestretch ratios are recorded based on parameterizations of the actuator's geometry. To demonstrate its application in practical conditions, the multistable actuator is used to perform visual inspection in a confined space, light source tracking during photovoltaic energy harvesting, and agile crawling.

Unzipped carbon nanotubes assisted 3D printable functionalized chitosan hydrogels for strain sensing applications.

Developing multifunctional hydrogels for wearable strain sensors has received significant attention due to their diverse applications, including human motion detection, personalized healthcare, soft robotics, and human-machine interfaces. However, integrating the required characteristics into one component remains challenging. To overcome these limitations, we synthesized multifunctional hydrogels using carboxymethyl chitosan (CMCS) and unzipped carbon nanotubes (f-CNTs) as strain sensor via a one-pot strategy. The polar groups in CMCS and f-CNTs enhance the properties of the hydrogels through different interactions. The hydrogels show superior printability with a uniformity factor (U) of 0.996 ± 0.049, close to 1. The f-CNTs-assisted hydrogels showed improved storage modulus (8.8 × 105 Pa) than the pure polymer hydrogel. The hydrogels adequately adhered to different surfaces, including human skin, plastic, plastic/glass interfaces, and printed polymers. The hydrogels demonstrated rapid self-healing and good conductivity. The biocompatibility of the hydrogels was assessed using human fibroblast cells. No adverse effects were observed with hydrogels, showing their biocompatibility. Furthermore, hydrogels exhibited antibacterial potential against Escherichia coli. The developed hydrogel exhibited unidirectional motion and complex letter recognition potential with a strain sensitivity of 2.4 at 210 % strain. The developed hydrogels could explore developing wearable electronic devices for detecting human motion.

Polyphenolic Carbon Quantum Dots with Intrinsic Reactive Oxygen Species Amplification for Two-Photon Bioimaging and In Vivo Tumor Therapy.

Recent studies indicate that mitochondrial dysfunctions and DNA damage have a critical influence on cell survival, which is considered one of the therapeutic targets for cancer therapy. In this study, we demonstrated a comparative study of the effect of polyphenolic carbon quantum dots (CQDs) on in vitro and in vivo antitumor efficacy. Dual emissive (green and yellow) shape specific polyphenolic CQDs (G-CQDs and Y-CQDs) were synthesized from easily available nontoxic precursors (phloroglucinol), and the antitumor property of the as-synthesized probe was investigated as compared to round-shaped blue emissive CQDs (B-CQDs) derived from well-reported precursor citric acid and urea. The B-CQDs had a nuclei-targeting property, and G-CQDs and Y-CQDs had mitochondria-targeting properties. We have found that the polyphenol containing CQDs (at a dose of 100 µg mL-1) specifically attack mitochondria by excess accumulation, altering the metabolism, inhibiting branching pattern, imbalanced Bax/Bcl-2 homeostasis, and ultimately generating oxidative stress levels, leading to oxidative stress-induced cell death in cancer cells in vitro. We show that G-CQDs are the main cause of oxidative stress in cancer cells because of their ability to produce sufficient •OH- and 1O2 radicals, evidenced by electron paramagnetic resonance spectroscopy and a terephthalic acid test. Moreover, the near-infrared absorption properties of the CQDs were exhibited in two-photon (TP) emission, which was utilized for TP cellular imaging of cancer cells without photobleaching. The in vivo antitumor test further discloses that intratumoral injection of G-CQDs can significantly augment the treatment efficacy of subcutaneous tumors without any adverse effects on BalB/c nude mice. We believe that shape-specific polyphenolic CQD-based nanotheranostic agents have a potential role in tumor therapy, thus proving an insight on treatment of malignant cancers.

Soft magnetic thin film deformation with a bistable electropermanent magnet.

Physically soft magnetic materials (PSMMs) represent an emerging class of materials that can change shape or rheology in response to an external magnetic field. However, until now, no studies have investigated using an electropermanent magnet (EPM) and magnetic repulsion to magnetically deform PSMMs. Such capabilities would enable the ability to deform PSMMs without the need for continuous electrical input and produce PSMM film deformation without an air gap, as would be required with magnetic attraction. To address this, we introduce a PSMM-EPM architecture in which the shape of a soft deformable thin film is controlled by switching between bistable on/off states of the EPM circuit. We characterized the deflection of a PSMM thin film when placed at controlled distances normal to the surface of the EPM and compared its response for cases when the EPM is in the 'on' and 'off' states. This work is the first to demonstrate a magnetically repelled soft deformable thin film that achieves two electronically-controlled modes of deformation through the on and off states of an EPM. This work has the potential to advance the development of new magneto-responsive soft materials and systems.

Multimaterial Printing of Liquid Crystal Elastomers with Integrated Stretchable Electronics.

Liquid crystal elastomers (LCEs) have grown in popularity in recent years as a stimuli-responsive material for soft actuators and shape reconfigurable structures. To make these material systems electrically responsive, they must be integrated with soft conductive materials that match the compliance and deformability of the LCE. This study introduces a design and manufacturing methodology for combining direct ink write (DIW) 3D printing of soft, stretchable conductive inks with DIW-based "4D printing" of LCE to create fully integrated, electrically responsive, shape programmable matter. The conductive ink is composed of a soft thermoplastic elastomer, a liquid metal alloy (eutectic gallium indium, EGaIn), and silver flakes, exhibiting both high stretchability and conductivity (order of 105 S m-1). Empirical tuning of the LCE printing parameters gives rise to a smooth surface (<10 µm) for patterning the conductive ink with controlled trace dimensions. This multimaterial printing method is used to create shape reconfigurable LCE devices with on-demand circuit patterning that could otherwise not be easily fabricated through traditional means, such as an LCE bending actuator able to blink a Morse code signal and an LCE crawler with an on/off photoresistor controller. In contrast to existing fabrication methodologies, the inclusion of the conductive ink allows for stable power delivery to surface mount devices and Joule heating traces in a highly dynamic LCE system. This digital fabrication approach can be leveraged to push LCE actuators closer to becoming functional devices, such as shape programmable antennas and actuators with integrated sensing.

Nanocellulose-assisted 3D-printable, transparent, bio-adhesive, conductive, and biocompatible hydrogels as sensors and moist electric generators.

Transparent hydrogels have found increasing applications in wearable electronics, printable devices, and tissue engineering. Integrating desired properties, such as conductivity, mechanical strength, biocompatibility, and sensitivity, in one hydrogel remains challenging. To address these challenges, multifunctional hydrogels of methacrylate chitosan, spherical nanocellulose, and ß-glucan with distinct physicochemical characteristics were combined to develop multifunctional composite hydrogels. The nanocellulose facilitated the self-assembly of the hydrogel. The hydrogels exhibited good printability and adhesiveness. Compared with the pure methacrylated chitosan hydrogel, the composite hydrogels exhibited improved viscoelasticity, shape memory, and conductivity. The biocompatibility of the composite hydrogels was monitored using human bone marrow-derived stem cells. Their motion-sensing potential was analyzed on different parts of the human body. The composite hydrogels also possessed temperature-responsiveness and moisture-sensing abilities. These results suggest that the developed composite hydrogels demonstrate excellent potential to fabricate 3D-printable devices for sensing and moist electric generator applications.

Recent Advances in Centrifugal Spinning and Their Applications in Tissue Engineering.

Over the last decade, researchers have investigated the potential of nano and microfiber scaffolds to promote wound healing, tissue regeneration, and skin protection. The centrifugal spinning technique is favored over others due to its relatively straightforward mechanism for producing large quantities of fiber. Many polymeric materials have yet to be investigated in search of those with multifunctional properties that would make them attractive in tissue applications. This literature presents the fundamental process of fiber generation, and the effects of fabrication parameters (machine, solution) on the morphologies such as fiber diameter, distribution, alignment, porous features, and mechanical properties. Additionally, a brief discussion is presented on the underlying physics of beaded morphology and continuous fiber formation. Consequently, the study provides an overview of the current advancements in centrifugally spun polymeric fiber-based materials and their morphological features, performance, and characteristics for tissue engineering applications.

Multifunctional 3D platforms for rapid hemostasis and wound healing: Structural and functional prospects at biointerfaces.

354Fabrication of multifunctional hemostats is indispensable against chronic blood loss and accelerated wound healing. Various hemostatic materials that aid wound repair or rapid tissue regeneration has been developed in the last 5 years. This review provides an overview of the three-dimensional (3D) hemostatic platforms designed through the latest technologies like electrospinning, 3D printing, and lithography, solely or in combination, for application in rapid wound healing. We critically discuss the pivotal role of micro/nano-3D topography and biomaterial properties in mediating rapid blood clots and healing at the hemostat-biointerface. We also highlight the advantages and limitations of the designed 3D hemostats. We anticipate that this review will guide the fabrication of smart hemostats of the future for tissue engineering applications.

Autonomous self-burying seed carriers for aerial seeding.

Aerial seeding can quickly cover large and physically inaccessible areas1 to improve soil quality and scavenge residual nitrogen in agriculture2, and for postfire reforestation3-5 and wildland restoration6,7. However, it suffers from low germination rates, due to the direct exposure of unburied seeds to harsh sunlight, wind and granivorous birds, as well as undesirable air humidity and temperature1,8,9. Here, inspired by Erodium seeds10-14, we design and fabricate self-drilling seed carriers, turning wood veneer into highly stiff (about 4.9 GPa when dry, and about 1.3 GPa when wet) and hygromorphic bending or coiling actuators with an extremely large bending curvature (1,854 m-1), 45 times larger than the values in the literature15-18. Our three-tailed carrier has an 80% drilling success rate on flat land after two triggering cycles, due to the beneficial resting angle (25°-30°) of its tail anchoring, whereas the natural Erodium seed's success rate is 0%. Our carriers can carry payloads of various sizes and contents including biofertilizers and plant seeds as large as those of whitebark pine, which are about 11 mm in length and about 72 mg. We compare data from experiments and numerical simulation to elucidate the curvature transformation and actuation mechanisms to guide the design and optimization of the seed carriers. Our system will improve the effectiveness of aerial seeding to relieve agricultural and environmental stresses, and has potential applications in energy harvesting, soft robotics and sustainable buildings.

Cellulose nanocrystals vs. cellulose nanospheres: A comparative study of cytotoxicity and macrophage polarization potential.

Nanocellulose application has been increasing owing to its appealing physicochemical properties. Monitoring of the crystallinity, surface topography, and reactivity of this high-aspect-ratio nanomaterial is crucial for efficient tissue engineering. Controlling macrophage polarization phenotype remains a challenge in regenerative medicine and tissue engineering. Herein, we monitored the effects of shape-regulated (rod and spherical) nanocellulose on the macrophage modulatory potential of RAW 246.7 cells in vitro. Spherical nanocellulose (s-NC) exhibited higher thermal stability and biocompatibility than rod nanocellulose. Macrophage polarization was profoundly affected by nanocellulose topography and incubation period. M2 polarization was observed in vitro after 1 day of treatment with s-NC, followed by M1 polarization after treatment for longer periods. Transcriptome analysis similarly revealed that M1 polarization was dominant after 1 day h of incubation with both nanocellulose types. These findings demonstrate that macrophage polarization can be controlled by selecting suitable nanocellulose shape and incubation time for desired applications.

Transcriptomic Changes toward Osteogenic Differentiation of Mesenchymal Stem Cells on 3D-Printed GelMA/CNC Hydrogel under Pulsatile Pressure Environment.

Biomimetic soft hydrogels used in bone tissue engineering frequently produce unsatisfactory outcomes. Here, it is investigated how human bone-marrow-derived mesenchymal stem cells (hBMSCs) differentiated into early osteoblasts on remarkably soft 3D hydrogel (70 ± 0.00049 Pa). Specifically, hBMSCs seeded onto cellulose nanocrystals incorporated methacrylate gelatin hydrogels are subjected to pulsatile pressure stimulation (PPS) of 5-20 kPa for 7 days. The PPS stimulates cellular processes such as mechanotransduction, cytoskeletal distribution, prohibition of oxidative stress, calcium homeostasis, osteogenic marker gene expression, and osteo-specific cytokine secretions in hBMSCs on soft substrates. The involvement of Piezo 1 is the main ion channel involved in mechanotransduction. Additionally, RNA-sequencing results reveal differential gene expression concerning osteogenic differentiation, bone mineralization, ion channel activity, and focal adhesion. These findings suggest a practical and highly scalable method for promoting stem cell commitment to osteogenesis on soft matrices for clinical reconstruction.

Highly stretchable, adhesive, and biocompatible hydrogel platforms of tannic acid functionalized spherical nanocellulose for strain sensors.

The development of multifunctional wearable electronic devices has received considerable attention because of their attractive applications. However, integrating multifunctional abilities into one component remains a challenge. To address this, we have developed a tannic acid-functionalized spherical nanocellulose/polyvinyl alcohol composite hydrogel using borax as a crosslinking agent for strain-sensing applications. The hydrogel demonstrates improved mechanical and recovery strengths and maintains its mechanical strength under freezing conditions. The hydrogels show ultra-stretching, adhesive, self-healing, and conductive properties, making them ideal candidates for developing strain-based wearable devices. The hydrogel exhibits good sensitivity with a 4.75 gauge factor. The cytotoxicity of the developed hydrogels was monitored with human dermal fibroblast cells by WST-8 assay in vitro. The antibacterial potential of the hydrogels was evaluated using Escherichia coli. The hydrogels demonstrate enhanced antibacterial ability than the control. Therefore, the developed multifunctional hydrogels with desirable properties are promising platforms for strain sensor devices.

Electrically stimulated 3D bioprinting of gelatin-polypyrrole hydrogel with dynamic semi-IPN network induces osteogenesis via collective signaling and immunopolarization.

In recent years, three-dimensional (3D) bioprinting of conductive hydrogels has made significant progress in the fabrication of high-resolution biomimetic structures with gradual complexity. However, the lack of an effective cross-linking strategy, ideal shear-thinning, appropriate yield strength, and higher print fidelity with excellent biofunctionality remains a challenge for developing cell-laden constructs, hindering the progress of extrusion-based 3D printing of conductive polymers. In this study, a highly stable and conductive bioink was developed based on polypyrrole-grafted gelatin methacryloyl (GelMA-PPy) with a triple cross-linking (thermo-photo-ionically) strategy for direct ink writing-based 3D printing applications. The triple-cross-linked hydrogel with dynamic semi-inner penetrating polymer network (semi-IPN) displayed excellent shear-thinning properties, with improved shape fidelity and structural stability during 3D printing. The as-fabricated hydrogel ink also exhibited "plug-like non-Newtonian" flow behavior with minimal disturbance. The bioprinted GelMA-PPy-Fe hydrogel showed higher cytocompatibility (93%) of human bone mesenchymal stem cells (hBMSCs) under microcurrent stimulation (250 mV/20 min/day). Moreover, the self-supporting and tunable mechanical properties of the GelMA-PPy bioink allowed 3D printing of high-resolution biological architectures. As a proof of concept, we printed a full-thickness rat bone model to demonstrate the structural stability. Transcriptomic analysis revealed that the 3D bioprinted hBMSCs highly expressed gene hallmarks for NOTCH/mitogen-activated protein kinase (MAPK)/SMAD signaling while down-regulating the Wnt/ß-Catenin and epigenetic signaling pathways during osteogenic differentiation for up to 7 days. These results suggest that the developed GelMA-PPy bioink is highly stable and non-toxic to hBMSCs and can serve as a promising platform for bone tissue engineering applications.

A Method for 3D Printing and Rapid Prototyping of Fieldable Untethered Soft Robots.

Because they are made of elastically deformable and compliant materials, soft robots can passively change shape and conform to their environment, providing potential advantages over traditional robotics approaches. However, existing manufacturing workflows are often labor intensive and limited in their ability to create highly integrated three-dimensional (3D) heterogeneous material systems. In this study, we address this with a streamlined workflow to produce field-deployable soft robots based on 3D printing with digital light processing (DLP) of silicone-like soft materials. DLP-based 3D printing is used to create soft actuators (2.2 g) capable of exerting up to 0.5 Newtons of force that are integrated into a bioinspired untethered soft robot. The robot walks underwater at speeds comparable with its biological analog, the brittle star. Using a model-free planning algorithm and feedback, the robot follows remote commands to move to desired positions. Moreover, we show that the robot is able to perform untethered locomotion outside of a laboratory and in a natural aquatic environment. Our results represent progress in soft robot manufacturing autonomy for a 3D printed untethered soft robot.

Recent Advances in 3D Printing of Photocurable Polymers: Types, Mechanism, and Tissue Engineering Application.

The conversion of liquid resin into solid structures upon exposure to light of a specific wavelength is known as photopolymerization. In recent years, photopolymerization-based 3D printing has gained enormous attention for constructing complex tissue-specific constructs. Due to the economic and environmental benefits of the biopolymers employed, photo-curable 3D printing is considered an alternative method for replacing damaged tissues. However, the lack of suitable bio-based photopolymers, their characterization, effective crosslinking strategies, and optimal printing conditions are hindering the extensive application of 3D printed materials in the global market. This review highlights the present status of various photopolymers, their synthesis, and their optimization parameters for biomedical applications. Moreover, a glimpse of various photopolymerization techniques currently employed for 3D printing is also discussed. Furthermore, various naturally derived nanomaterials reinforced polymerization and their influence on printability and shape fidelity are also reviewed. Finally, the ultimate use of those photopolymerized hydrogel scaffolds in tissue engineering is also discussed. Taken together, it is believed that photopolymerized 3D printing has a great future, whereas conventional 3D printing requires considerable sophistication, and this review can provide readers with a comprehensive approach to developing light-mediated 3D printing for tissue-engineering applications.

3D Printing of Liquid Metal Embedded Elastomers for Soft Thermal and Electrical Materials.

Liquid metal embedded elastomers (LMEEs) are composed of a soft polymer matrix embedded with droplets of metal alloys that are liquid at room temperature. These soft matter composites exhibit exceptional combinations of elastic, electrical, and thermal properties that make them uniquely suited for applications in flexible electronics, soft robotics, and thermal management. However, the fabrication of LMEE structures has primarily relied on rudimentary techniques that limit patterning to simple planar geometries. Here, we introduce an approach for direct ink write (DIW) printing of a printable LMEE ink to create three-dimensional shapes with various designs. We use eutectic gallium-indium (EGaIn) as the liquid metal, which reacts with oxygen to form an electrically insulating oxide skin that acts as a surfactant and stabilizes the droplets for 3D printing. To rupture the oxide skin and achieve electrical conductivity, we encase the LMEE in a viscoelastic polymer and apply acoustic shock. For printed composites with a 80% LM volume fraction, this activation method allows for a volumetric electrical conductivity of 5 × 104 S cm-1 (80% LM volume)─significantly higher than what had been previously reported with mechanically sintered EGaIn-silicone composites. Moreover, we demonstrate the ability to print 3D LMEE interfaces that provide enhanced charge transfer for a triboelectric nanogenerator (TENG) and improved thermal conductivity within a thermoelectric device (TED). The 3D printed LMEE can be integrated with a highly soft TED that is wearable and capable of providing cooling/heating to the skin through electrical stimulation.

Plant-Actuated Micro-Nanorobotics Platforms: Structural Designs, Functional Prospects, and Biomedical Applications.

Plants are anatomically and physiologically different from humans and animals; however, there are several possibilities to utilize the unique structures and physiological systems of plants and adapt them to new emerging technologies through a strategic biomimetic approach. Moreover, plants provide safe and sustainable results that can potentially solve the problem of mass-producing practical materials with hazardous and toxic side effects, particularly in the biomedical field, which requires high biocompatibility. In this review, it is investigated how micro-nanostructures available in plants (e.g., nanoparticles, nanofibers and their composites, nanoporous materials, and natural micromotors) are adapted and utilized in the design of suitable materials for a micro-nanorobot platform. How plants' work on micro- and nanoscale systems (e.g., surface roughness, osmotically induced movements such as nastic and tropic, and energy conversion and harvesting) that are unique to plants, can provide functionality on the platform and become further prospective resources are examined. Furthermore, implementation across organisms and fields, which is promising for future practical applications of the plant-actuated micro-nanorobot platform, especially on biomedical applications, is discussed. Finally, the challenges following its implementation in the micro-nanorobot platform are also presented to provide advanced adaptation in the future.

Bioengineered Lab-Grown Meat-like Constructs through 3D Bioprinting of Antioxidative Protein Hydrolysates.

Lab-grown bovine meat analogues are emerging alternatives to animal sacrifices for cultured meat production. The most challenging aspect of the production process is the rapid proliferation of cells and establishment of the desired 3D structure for mass production. In this study, we developed a direct ink writing-based 3D-bioprinted meat culture platform composed of 6% (w/v) alginate and 4% (w/v) gelatin (Alg/Gel)-based hydrogel scaffolds supplemented with naturally derived protein hydrolysates (PHs; 10%) from highly nutritive plants (soybean, pigeon pea, and wheat), and some selected edible insects (beetles, crickets, and mealworms) on in vitro proliferation of bovine myosatellite cells (bMSCs) extracted from fresh meat samples. The developed bioink exhibited excellent shear-thinning behavior (n < 1) and mechanical stability during 3D bioprinting. Commercial proteases (Alcalase, Neutrase, and Flavourzyme) were used for protein hydrolysis. The resulting hydrolysates exhibited lower-molecular-weight bands (12-50 kDa) than those of crude isolates (55-160 kDa), as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The degree of hydrolysis was higher in the presence of Alcalase for both plant (34%) and insect (62%) PHs than other enzymes. The 3D-printed hydrogel scaffolds displayed excellent bioactivity and stability after 7 days of incubation. The developed prototype structure (pepperoni meat, 20 × 20 × 5 mm) provided a highly stable, nutritious, and mechanically strong structure that supported the rapid proliferation of myoblasts in a low-serum environment during the entire culture period. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay enhanced the free radical reduction of Alcalase- and Neutrase-treated PHs. Furthermore, the bioprinted bMSCs displayed early myogenesis (desmin and Pax7) in the presence of PHs, suggesting its role in bMSC differentiation. In conclusion, we developed a 3D bioprinted and bioactive meat culture platform using Alg/Gel/PHs as a printable and edible component for the mass production of cultured meat.

Liquid Crystal Elastomer with Integrated Soft Thermoelectrics for Shape Memory Actuation and Energy Harvesting.

Liquid crystal elastomers (LCEs) have attracted tremendous interest as actuators for soft robotics due to their mechanical and shape memory properties. However, LCE actuators typically respond to thermal stimulation through active Joule heating and passive cooling, which make them difficult to control. In this work, LCEs are combined with soft, stretchable thermoelectrics to create transducers capable of electrically controlled actuation, active cooling, and thermal-to-electrical energy conversion. The thermoelectric layers are composed of semiconductors embedded within a 3D printed elastomer matrix and wired together with eutectic gallium-indium (EGaIn) liquid metal interconnects. This layer is covered on both sides with LCE, which alternately heats and cools to achieve cyclical bending actuation in response to voltage-controlled Peltier activation. Moreover, the thermoelectric layer can harvest energy from thermal gradients between the two LCE layers through the Seebeck effect, allowing for regenerative energy harvesting. As demonstrations, first, closed-loop control of the transducer is performed to rapidly track a changing actuator position. Second, a soft robotic walker that is capable of walking toward a heat source and harvesting energy is introduced. Lastly, phototropic-inspired autonomous deflection of the limbs toward a heat source is shown, demonstrating an additional method to increase energy recuperation efficiency for soft systems.