Determination of Aldicarb, Carbofuran and Methamidophos in Blood Derived from Forensic Cases through Liquid Chromatography with Electrospray Ionization and Tandem Mass Spectrometry (LC--ESI-MS-MS).

The presence and use of carbamate and organophosphate pesticides with agricultural and urban purposes in Colombia has been justified for pest control. However, these substances pose a national problem because of their toxic nature, which is associated with accidental poisoning or even with homicides or suicides related to acute fatal poisoning. This study aims to develop and to validate an analytical methodology for the determination of the aldicarb, carbofuran and methamidophos pesticides in blood through liquid chromatography--tandem mass spectrometry (LC-MS-MS). To this end, the method for extracting pesticides from the blood was developed, the conditions of LC were defined, the instrumental system MS-MS was optimized and the bioanalytical methodology was validated. This methodology proved to be selective, precise, accurate and linear in the concentration range from 0.10 to 5.0 µg/mL, with a limit of detection of 0.020 µg/mL for aldicarb and carbofuran and 0.050 µg/mL for methamidophos, recovery between 90% and 102%, and stability at room temperature and in the autosampler between 80% and 120%. The analytical methodology was applied to 34 forensic cases. Carbofuran was found at a concentration ranging from 0.020 to >5.0 µg/mL, aldicarb was found at a concentration ranging from 0.10 to 2.5 µg/mL and methamidophos was found at a concentration >5.0 µg/mL. In 62% of the cases, the pesticides under study were used to commit suicide. Necropsy findings of pesticide poisoning are non-specific. Therefore, toxicological blood analysis provides significant information at the forensic level, and the analytical method validated represents a sensitive, fast and reliable analysis with little solvent consumption of a small sample amount, so it is suitable for routine application in fatal pesticide poisonings.

Un acercamiento desde la ecología social a la lectura de territorio en la intervención del trabajo social / An approach from social ecology to the reading of territory in social work intervention

Este artículo de reflexión desarrolla la siguiente premisa: realizar lectura de territorio para llevar a cabo procesos de gestión ambiental y planificación territorial, como un resultado de la investigación realizada en el 2018, Prácticas y saberes de intervención del Trabajo Social en el escenario ambiental; desde la experiencia de los departamentos de Antioquia y Caldas. El presente artículo pretende entonces abordar el papel que asume el trabajador social en relación con la intervención en el escenario ambiental, partiendo del reconocimiento y la lectura del territorio, entendido este no solo como un espacio geográfico, sino también como un espacio donde convergen diferentes aristas, tales como los ámbitos sociales y culturales inmersos en la cotidianidad del hombre; con ello se espera generar reflexiones y movilizaciones frente al reconocimiento de derechos e incentivar la participación ciudadana. Teniendo en cuenta este panorama, y pensando desde la ecología social, es indispensable que el ser humano comprenda el vínculo que tiene con la naturaleza, no solo desde el enfoque natural, sino también desde el social y el cultural.

This reflection article develops the following premise: to perform territory reading to carry out processes of environmental management and territorial planning, as a result of the research conducted in 2018, Practices and knowledge of Social Work intervention in the environmental scenario; from the experience of the departments of Antioquia and Caldas. This article aims then to address the role assumed by the social worker in relation to the intervention in the environmental scenario, starting from the recognition and reading of the territory, understood not only as a geographical space, but also as a space where different edges converge, such as the social and cultural spheres immersed in the daily life of man; with this, it is expected to generate reflections and mobilizations in front of the recognition of rights and encourage citizen participation. Considering this panorama, and thinking from the perspective of social ecology, it is essential that human beings understand the link they have with nature, not only from the natural approach, but also from the social and cultural ones.