[Bartonella henselae in children with regional adenitis treated in a Peruvian national hospital, 2012]. / Bartonella henselae en niños con adenitis regional atendidos en un hospital nacional del Perú, 2012.

In order to determine the frequency of seropositive cases of Bartonella henselae in children with regional adenitis treated in a national hospital in Peru, a cross-sectional study was conducted in 106 children with regional adenitis greater than 1 cm in diameter. The sample was selected from patients aged 5-11 years seen at the National Institute of Child Health for acute onset of regional adentitis, with more than five days of symptoms. B. henselae seropositivity was defined by indirect immunofluorescence test. We found that 86 children (81.1%) were positive for B.henselae. The median age of the patients was 7 years. In the bivariate analysis, the following associated factors were found: aged 5 years, history of fever, lymphadenopathy greater than 4 cm and reported contact with cat. In conclusion, children with regional adenitis treated in this national referral hospital showed a high frequency of positive serology for B. henselae.

Bartonella henselae en niños con adenitis regional atendidos en un hospital nacional del Perú, 2012 / Bartonella henselae in children with regional adenitis treated in a Peruvian national hospital, 2012

Con el objetivo de determinar la frecuencia de casos seropositivos a Bartonella henselae en niños con adenitis regional atendidos en un hospital nacional del Perú, se realizó un estudio trasversal en 106 niños con adenitis regional mayor de 1 cm de diámetro, de aparición aguda, con tiempo de enfermedad mayor de cinco días, atendidos en el Instituto Nacional de Salud del Niño durante el año 2012. Se definió seropositividad para B. henselae mediante el examen de inmunofluorescencia indirecta, siendo positivos 86 niños (81,1%) con una mediana de edad de 7 años, rango de 5 a 11; en el análisis bivariado se encontraron como factores asociados, edad mayor de 5 años, antecedentes de fiebre, adenopatía mayor de 4 cm y reporte de contacto con gato. En conclusión, los niños con adenitis regional atendidos en este hospital de referencia nacional presentaron una frecuencia alta de serología positiva para B. henselae.

In order to determine the frequency of seropositive cases of Bartonella henselae in children with regional adenitis treated in a national hospital in Peru, a cross-sectional study was conducted in 106 children with regional adenitis greater than 1 cm in diameter. The sample was selected from patients aged 5-11 years seen at the National Institute of Child Health for acute onset of regional adentitis, with more than five days of symptoms. B. henselae seropositivity was defined by indirect immunofluorescence test. We found that 86 children (81.1%) were positive for B.henselae. The median age of the patients was 7 years. In the bivariate analysis, the following associated factors were found: aged 5 years, history of fever, lymphadenopathy greater than 4 cm and reported contact with cat. In conclusion, children with regional adenitis treated in this national referral hospital showed a high frequency of positive serology for B. henselae.

Bartonella henselae en niños con adenitis regional atendidos en un hospital nacional del Perú, 2012 / Bartonella henselae in children with regional adenitis treated in a peruvian national hospital, 2012

Con el objetivo de determinar la frecuencia de casos seropositivos a Bartonella henselae en niños con adenitis regional atendidos en un hospital nacional del Perú, se realizó un estudio trasversal en 106 niños con adenitis regional mayor de 1 cm de diámetro, de aparición aguda, con tiempo de enfermedad mayor de cinco días, atendidos en el Instituto Nacional de Salud del Niño durante el año 2012. Se definió seropositividad para B. henselae mediante el examen de inmunofluorescencia indirecta, siendo positivos 86 niños (81,1%) con una mediana de edad de 7 años, rango de 5 a 11; en el análisis bivariado se encontraron como factores asociados, edad mayor de 5 años, antecedentes de fiebre, adenopatía mayor de 4 cm y reporte de contacto con gato. En conclusión, los niños con adenitis regional atendidos en este hospital de referencia nacional presentaron una frecuencia alta de serología positiva para B. henselae. (AU)

In order to determine the frequency of seropositive cases of Bartonella henselae in children with regional adenitis treated in a national hospital in Peru, a cross-sectional study was conducted in 106 children with regional adenitis greater than 1 cm in diameter. The sample was selected from patients aged 5-11 years seen at the National Institute of Child Health for acute onset of regional adentitis, with more than five days of symptoms. B. henselae seropositivity was defined by indirect immunofluorescence test. We found that 86 children (81.1%) were positive for B.henselae. The median age of the patients was 7 years. In the bivariate analysis, the following associated factors were found: aged 5 years, history of fever, lymphadenopathy greater than 4 cm and reported contact with cat. In conclusion, children with regional adenitis treated in this national referral hospital showed a high frequency of positive serology for B. henselae. (AU)

Actinomycetes bioactivos de sedimento marino de la costa central del Perú / Bioactives Actinomycetes of marine sediment from the central coast of Peru

En el presente trabajo evaluamos la actividad antibacteriana y antifúngica de actinomycetes marinos sobre patógenos de origen clínico. Asimismo, fueron evaluadas la capacidad de producir enzimas extracelulares como carbohidrasas, lipasas y proteasas. Los Actinomycetes fueron aislados de sedimentos colectados entre setiembre a diciembre del 2005 de las Bahías de Ancón (Lima) e Independencia (Ica) de 34 y 100 m de profundidad. El aislamiento se realizó en Agar Caseína - Almidón (ACA) y Agar Marino (AM) con adición de Cicloheximide (10 ng/mL). Las evaluaciones antimicrobianas fueron realizadas frente a bacterias patógenas antibiótico-multirresistentes y hongos de origen clínico; en tanto, para evaluar su actividad multienzimática se utilizaron sustratos poliméricos diversos. Se aislaron un total de 62 actinomycetes, de los cuales 31 (50 por ciento) mostraron actividad antibacteriana frente a Staphylococcus aureus, 36 (59 por ciento) frente a Pseudomonas aeruginosa y 23 (37 por ciento) a ambos patógenos. Las cepas de actinomycetes I-400A y M10-77 identificadas en cada caso como Streptomyces y Thermoactinomyces fueron las que exhibieron mayor actividad inhibitoria frente a P. aeruginosa y S. aureus respectivamente. Asimismo, 13 actinomycetes (20,97 por ciento) mostraron actividad antifúngica frente a cultivos de Candida albicans cepa 1511 y 17 (27,42 por ciento) frente a Candida albicans cepa 1511MIC; sin embargo, ningún actinomycete presentó actividad inhibitoria frente a Aspergillus niger, Aspergillus fumigatus y Trichophyton mentagrophytes. La mayoría de los actinomycetes mostraron tener actividad multienzimática capaz de hidrolizar compuestos poliméricos como el tween-80 (96 por ciento), la gelatina (95 por ciento), almidón (93 por ciento), lecitina (88 por ciento) y la caseína (74 por ciento).

In the present research we evaluated the antibacterial and antifungical activity of marine actinomycetes over pathogen of clinical origin. Likewise, it was evaluated the capacity to produce extracellular enzymes like carbohidrases, lipases and proteases. The Actinomycetes were isolated from sediments collected between September to December 2005 of Ancón (Lima) and Independencia (Ica) Bays at depths of 34 and 100 m. The isolation was performed in Casein - Starch Agar (CSA) and Marine Agar (AM) with addition of Cicloheximide (10 ng/mL). The antimicrobial evaluations were done comparing them with pathogenic antibiotic-multiresistant bacteria and fungi from clinical origin; in as much, to evaluate their multienzimatic activity several polimeric substrates were used. A total of 62 actinomycetes were isolated, 31 of there (50 per cent) showed antibacterial activity in opposite to Staphylococcus aureus, 36 (59 per cent) in opposite to Pseudomonas aeruginosa and 23 (37 per cent) to both pathogens. Strains of actinomycetes I-400A and M10-77 identified in each case like Streptomyces and Thermoactinomyces exhibited higher inhibitory activity against S. aureus and P. aeruginosa respectively. Also, 13 actinomycetes (20,97 per cent) showed to antifungical activity against cultures of Candida albicans strain 1511 and 17 (27,42 per cent) with Candida albicans strain 1511MIC; nevertheless, no actinomycete displayed inhibitory activity to the growth of Aspergillus niger, Aspergillus fumigatus and Trichophyton mentagrophytes. Most Actinomycetes showed to have multienzymic activity able to hydrolysis polymerics compounds like the tween-80 (96 per cent), gelatin (95 per cent), starch (93 per cent), lecitine (88 per cent) and casein (74 per cent).