Effect of sodium nitroprusside on physiological and anatomical features of salt-stressed Raphanus sativus.

Sodium nitroprusside (SNP), which produces nitric oxide (NO) has the well-documented potential to alleviate the adverse effects of various abiotic stressors such as salinity. The present study aimed at investigating how the application of SNP can ameliorate the adverse effects of salt stress and boost tolerance in Raphanus sativus. Salt stress induced by application of 100 or 200 mM NaCl significantly decreased photosynthetic pigments and chlorophyll fluorescence, followed by a significant reduction in carbohydrate content. SNP treatment increased salt-tolerance in plants by inhibiting the adverse effect of salinity on the photosynthetic apparatus and the accumulation of sugars. Salt stress was accompanied by a reduction in total antioxidant power (FRAP), accumulation of damaging levels of H2O2, lipid peroxidation, and reduction in protein, while SNP enhanced FRAP, reduced H2O2 and lipid peroxidation, and restored protein abundance. SNP treatment also increased hypocotyl growth of salt-stressed plants, accompanied by improvement in anatomical structure. Cross sections of the hypocotyl showed increased diameter of the central cylinder and thickness of the casparian strip in the SNP-treated plants under stress conditions. Indeed, the observed improvement in the growth of hypocotyl and leaves of salt-stressed radish plants treated with SNP, in parallel with improved physiology and anatomical features, suggested that NO can regulate diverse mechanisms to effectively increase salt tolerance.

Cross-talk between nitric oxide, hydrogen peroxide and calcium in salt-stressed Chenopodium quinoa Willd. At seed germination stage.

Seed germination is critical for successful crop production and this growth stage can be very sensitive to salt stress depending on the plant's tolerance mechanisms. The pretreatment of Chenopodium quinoa (quinoa) seeds with CaCl2, H2O2 and sodium nitroprusside (SNP) limited the adverse effect of salt stress on seed germination. The pre-treated seeds showed a significant increase in germination rate, relative germination rate and germination index while the mean germination time was significantly reduced under both optimal and stress conditions. In parallel with seed germination, the negative effect of salt stress on the activity of α-amylase and ß-amylase was reduced in pre-treated seeds. The amylase enzymes are responsible for starch hydrolysis, so the reduction of amylase activity by salt stress resulted in higher starch content in the seeds and lower concentrations of water-soluble sugars such as glucose. Pretreatment stimulated amylase activity resulting in starch breakdown and increased content of water-soluble sugars in the salt-stressed seeds. Protein and amino acid contents were significantly enhanced in salt-stressed seeds, which were highlighted in pre-treated seeds. The findings of this study demonstrate that pretreatment of quinoa seeds with CaCl2, H2O2 and SNP at 5, 5 and 0.2 mM, respectively, concentration to achieve rapid germination at high levels under optimal and salt-stress conditions.