Antioxidant and anti-inflammatory activity of curcumin transdermal gel in an IL-10 knockout mouse model of inflammatory bowel disease.

Inflammatory bowel diseases are a group of inflammatory disorders of the gastrointestinal tract. Their prevalence is still low in Brazil, but the incidence is increasing annually. A variety of compounds present in Curcuma longa L., particularly curcumin, have been shown to reduce oxidative stress and aid in the prevention of associated diseases. This study aimed to assess the effect of curcumin transdermal gel on oxidative stress and intestinal inflammation in IL-10 knockout mice. Female mice were divided into four groups: a control group (C0) treated with vehicle and three experimental groups treated with transdermal gel containing 50 (C50), 75 (C75), and 100 (C100) mg curcumin kg-1 body weight. Colon malondialdehyde concentrations were lower in C50 and C75 groups. C100 treatment led to reduced catalase activity in the small intestine, whereas C50, C75, and C100 treatments resulted in decreased catalase activity in the colon. In contrast, superoxide dismutase activity increased in the small intestine of C50 and C75 mice and decreased in the colon of C50, C75, and C100 mice. Glutathione S-transferase activity increased in the small intestine and decreased in the colon of C75 animals. These findings suggest that curcumin transdermal gel exerts a protective effect against oxidative stress.

Dieta hiperlipídica e ácido linoleico conjugado: efeitos nos lipídios séricos, peso e composição corporal de camundongos Apo E(-/-) exercitados / High-fat diet and conjugated linoleic acid: effects on serun lipids, weight and body composition of exercised Apo E(-/-) mice

Estratégias têm sido utilizadas para a prevenção de doenças cardiovasculares e aumento de peso. Sendo assim, muito tem sido especulado sobre alimentos funcionais e seus efeitos benéficos para a saúde humana e, em especial do Ácido Linoleico Conjugado (CLA). O objetivo foi avaliar os efeitos da dieta hiperlipídica e do CLA sobre os lipídios séricos, peso e composição corporal de camundongos Apolipoproteina E(-/-) (Apo E) exercitados. Camundongos knockout para Apo E foram alocados em quatro grupos/dieta: Normal (n=5), Hiperlipídica (n=6), Normal+CLA (n=5) e Hiperlipídica+CLA (n=6). Todos os grupos foram submetidos a um protocolo de corrida em esteira. Determinou-se o colesterol total, LDL-c e HDL-c no sangue, o peso e a composição corporal. Utilizou-se ANOVA e Tukey ao nível de significância de 5%. A dieta hiperlipídica elevou o colesterol total (Hiperlipídica=920,2±392,3 e Normal=382,3±207,9), LDL-c (Hiperlipídica=893,9±402,9 e Normal=339,9±204,8) e o peso corporal (Hiperlipídica=25,83±1,90 e Normal=339,9±204,8). O CLA reduziu a gordura (CLA=4,24±1,82 e Sem CLA=6,28±2,77) e elevou a proteína (CLA=23,02±1,04 e Sem CLA=21,45±1,04) na carcaça. Concluiu-se que a dieta hiperlipídica aumenta colesterol total e LDL-c e, o consumo de CLA diminui o gordura e aumenta a proteína na carcaça de camundongos Apo E(-/-) exercitados.

Strategies have been used for prevention of cardiovascular disease and weight gain. So much has been talked about functional foods and their beneficial effects on human health and, in particular conjugated linoleic acid. Evaluate the effects of high-fat diet and CLA on serum lipids, weight and body composition in Apolipoprotein E (-/-) mice (Apo E) exercised. Knockout mice ApoE were divided into four groups/diet: Normal (n=5), High-fat (n = 6), Normal+CLA (n=5) and High-fat+CLA (n=6). All groups underwent a protocol of treadmill running. Total cholesterol, LDL-c and HDL-c in the serum, weight and body composition were measured ANOVA followed by Tukey test were used (P<0.05). The high-fat diet elevated total cholesterol (High-fat=920,2± 392,3 and Normal=382,3±207,9), LDL-c (High fat=893,9±402,9 and Normal=339,9±204,8) and body weight (High-fat=25,83±1,90 and Normal= 339,9±204,8). The CLA reduced fat (CLA=4,24±1,82 and Without CLA=6,28±2,77) and increased the protein (CLA=23,02±1,04 and Without CLA=21,45±1,04) in the carcass. We conclude that the High-fat diet increases total cholesterol and LDL-C, and the consumption of the CLA reduces fat and increases the protein in the body composition of exercised ApoE(-/-) mice.