Brain glucose hypometabolism and hippocampal inflammation in Goto-Kakizaki rats.

Brain glucose hypometabolism and neuroinflammation are early pathogenic manifestations in neurological disorders. Neuroinflammation may also disrupt leptin signaling, an adipokine that centrally regulates appetite and energy balance by acting on the hypothalamus and exerting neuroprotection in the hippocampus. The Goto-Kakizaki (GK) rat is a non-obese type 2 diabetes mellitus (T2DM) animal model used to investigate diabetes-associated molecular mechanisms without obesity jeopardizing effects. Wistar and GK rats received the maintenance adult rodent diet. Also, an additional control group of Wistar rats received a high-fat and high-sugar diet (HFHS) provided by free consumption of condensed milk. All diets and water were provided ad libitum for eight weeks. Brain glucose uptake was evaluated by 2-deoxy-2-[fluorine-18] fluoro-D-glucose under basal (saline administration) or stimulated (CL316,243, a selective ß3-AR agonist) conditions. The animals were fasted for 10-12 h, anesthetized, and euthanized. The brain was quickly dissected, and the hippocampal area was sectioned and stored at -80°C in different tubes for protein and RNA analyses on the same animal. GK rats exhibited attenuated brain glucose uptake compared to Wistar animals and the HFHS group under basal conditions. Also, the hippocampus of GK rats displayed upregulated leptin receptor, IL-1ß, and IL-6 gene expression and IL-1ß and the subunit of the transcription factor NF-κB (p-p65) protein expression. No significant alterations were detected in the hippocampus of HFHS rats. Our data indicated that a genetic predisposition to T2DM has significant brain deteriorating features, including brain glucose hypometabolism, neuroinflammation, and leptin signaling disruption in the hippocampal area.

Brain glucose hypometabolism and hippocampal inflammation in Goto-Kakizaki rats

Brain glucose hypometabolism and neuroinflammation are early pathogenic manifestations in neurological disorders. Neuroinflammation may also disrupt leptin signaling, an adipokine that centrally regulates appetite and energy balance by acting on the hypothalamus and exerting neuroprotection in the hippocampus. The Goto-Kakizaki (GK) rat is a non-obese type 2 diabetes mellitus (T2DM) animal model used to investigate diabetes-associated molecular mechanisms without obesity jeopardizing effects. Wistar and GK rats received the maintenance adult rodent diet. Also, an additional control group of Wistar rats received a high-fat and high-sugar diet (HFHS) provided by free consumption of condensed milk. All diets and water were provided ad libitum for eight weeks. Brain glucose uptake was evaluated by 2-deoxy-2-[fluorine-18] fluoro-D-glucose under basal (saline administration) or stimulated (CL316,243, a selective β3-AR agonist) conditions. The animals were fasted for 10-12 h, anesthetized, and euthanized. The brain was quickly dissected, and the hippocampal area was sectioned and stored at -80°C in different tubes for protein and RNA analyses on the same animal. GK rats exhibited attenuated brain glucose uptake compared to Wistar animals and the HFHS group under basal conditions. Also, the hippocampus of GK rats displayed upregulated leptin receptor, IL-1β, and IL-6 gene expression and IL-1β and the subunit of the transcription factor NF-κB (p-p65) protein expression. No significant alterations were detected in the hippocampus of HFHS rats. Our data indicated that a genetic predisposition to T2DM has significant brain deteriorating features, including brain glucose hypometabolism, neuroinflammation, and leptin signaling disruption in the hippocampal area.

Prevalência de teníase no município de Pedra Branca Estado do Ceará, Brasil / Prevalence of taeniasis in municipal district Pedra Branca of Ceará State, Brazil

A teníase é reconhecida como importante problema de saúde pública em regiões onde as condições sanitárias são precárias e em países industrializados que recebem imigrantes de regiões onde a infecção é endêmica. O homem, responsável pela manutenção do ciclo parasitário, é o único hospedeiro da forma adulta dos parasitos Taenia solium e Taenia saginata. Apesar da importância do porco no ciclo de vida da Taenia solium, pouca atenção é dada em relação à infecção suína e humana. Estudo epidemiológico realizado no município de Pedra Branca no período de 1997 a 1998 demonstrou alto índice de carne de porco com cisticercose. O objetivo do presente estudo foi investigar a prevalência de teníase na população de Pedra Branca a fim de identificar os casos de teníase. Para isso empregou-se o método de Kato modificado por Katz por ser de fácil execução e de nos fornecer preparações quase permanentes. Nesse estudo, foram analisadas 831 amostras, de indivíduos de ambos os sexos, na faixa etária de 02 a 70 anos, com 54 (6,5%) de positivas. Os resultados mostraram a prevalência de diferentes espécies de helmintos, onde a maior freqüência foi de Ascaris lumbricoides 19 (2,30%), seguido de ancilostomideos 12 (1,44%) e em terceiro de Taenia sp 10 (1,20%). A prevalência de teníase foi bastante significativa levando-se em consideração que o método utilizado não é específico para o diagnóstico de teníase.

Taeniasis are recognized as important public health problem in many developed countries for regions with bad sanitary conditions as well as for industrial countries in the cases of emigration from the regions with endemic infection. Human intestin is responsible for realization of the parasite cycle and is unique accommodation for adult parasite form: Taenia solium and Taenia saginata In spite of importance of pigs in the life cycle of Taenia solium, it was small attention to relation between human and pig infection. Epidemiological study accomplished in Pedra Branca in the period 1997–1998 demonstrated the high index of cysticercosis contamination of pork. The purpose of the present study was for measuring the prevalence of human taeniasis on the Pedra Branca population. For this it was used the Kato method, modificated by Katz in order to be easy in use and to supply the preparations quasi permanent. In this study were analyzed 831 samples of stool of persons of both sexes between 2 and 70 year, with 54 (6.5%) of positives. The results show prevalent of different types of helminths, where Ascaris lumbricoides 19 (2.30%) has the biggest index, second was ancilostomideos 12 (1.44%) and third Taenia sp 10 (1.20%). The prevalence of taeniasis was significant considering that the used method was not specific for it diagnostic.