RESUMO
O objetivo desse plano de trabalho foi avaliar criticamente a evidência atual sobre a associação entre erosão dental e asma em crianças. Foi realizada uma revisão sistemática com estudos observacionais transversais do tipo caso-controle em crianças de 3 a 12 anos de idade. A pesquisa foi conduzida por dois revisores independentes (Kappa>0,8), em cinco bancos de dados eletrônicos primários, além de uma pesquisa de literatura cinzenta. O risco de viés dos artigos incluídos foi realizado usando o Joanna Briggs Institute Critical. Lista de verificação de avaliação para estudos transversais analíticos. RevMan 5.4 foi usado para gerar figura do risco de viés e realizar a meta- análise. A qualidade da evidência foi avaliada de acordo com o Classificação de Recomendações Avaliação, Desenvolvimento e Avaliação (GRADE). Cinco estudos foram incluídos para análise qualitativa, dos quais quatro foram incluídos para análise quantitativa, com total de 2047 crianças. Três dos trabalhos apresentaram moderado risco de viés, um com alto risco, e um com baixo risco de viés. A confiança na evidência foi classificada como "muito baixa". Concluiu-se que não há associação entre asma e erosão dental.
The aim of this study was to critically evaluate the current evidence on the association between dental erosion and asthma in children. A systematic review was carried out with case-control cross-sectional observational studies in children aged 3 to 12 years. The search was conducted by two independent reviewers (Kappa>0.8), across five primary electronic databases, in addition to a gray literature search. The risk of bias of included articles was performed using the Joanna Briggs Institute Critical. Evaluation checklist for analytical cross-sectional studies. RevMan 5.4 was used to generate the risk of bias figure and perform a meta-analysis. The quality of the evidence was assessed according to the Assessment, Development and Evaluation (GRADE) Recommendations Rating. Five studies were included for qualitative analysis, of which four were included for quantitative analysis, with a total of 2047 children. Three of the studies adopted moderate risk of bias, one with high risk, and one with low risk of bias. Confidence in the evidence was rated "very low". It was concluded that there is no association between asthma and dental erosion.
El objetivo de este plan de trabajo fue evaluar críticamente la evidencia actual sobre la asociación entre erosión dental y asma en niños. Se realizó una revisión sistemática con estudios observacionales transversales de casos y controles en niños de 3 a 12 años. La búsqueda fue realizada por dos revisores independientes (Kappa>0,8), en cinco bases de datos electrónicas primarias, además de una búsqueda en la literatura gris. El riesgo de sesgo de los artículos incluidos se realizó mediante el Joanna Briggs Institute Critical. Lista de verificación de evaluación para estudios transversales analíticos. Se utilizó RevMan 5.4 para generar la cifra de riesgo de sesgo y realizar el metanálisis. La calidad de la evidencia se evaluó de acuerdo con la Clasificación de recomendaciones de evaluación, desarrollo y evaluación (GRADE). Se incluyeron cinco estudios para el análisis cualitativo, de los cuales cuatro se incluyeron para el análisis cuantitativo, con un total de 2047 niños. Tres de los artículos tenían riesgo moderado de sesgo, uno con alto riesgo y uno con bajo riesgo de sesgo. La confianza en la evidencia se calificó como "muy baja". Se concluyó que no existe asociación entre el asma y la erosión dental.
RESUMO
BACKGROUND: Dental erosion has become a relevant public health problem in recent years and is related to the increase in the consumption of acidic beverages. Objective: The aim of the present study was to evaluate the erosive potential of energy drinks on dental enamel using an in vitro erosion model. MATERIAL AND METHODS: Thirty-eight blocks of human enamel were divided into four groups: G1- TNT Energy Drink®(n=8), G2- Red Bull® (n=10), G3- Monster Energy® (n=10), and G4- Coca-Cola® (n=10) (positive control). For the chemical analysis, the pH values, titratable acidity, and buffering capacity of the beverages were measured in triplicate. For the erosive test, the specimens were immersed in the beverages (5ml/block) for 30 minutes at room temperature with gentle shaking. Initial and final surface microhardness values were measured and the percentage of the loss of surface microhardness was calculated. Profilometry (surface loss and lesion depth) and mineral loss analysis (quantitative light-induced fluorescence) were performed. The data were analysed statistically using ANOVA followed by the Bonferroni correction, Pearson's correlation test, and multiple linear regression (p<0.05). RESULTS: The energy drinks had pH values ranging from 2.36 to 3.41. The lowest titratable acidity value was recorded for Monster Energy® and the highest was recorded for TNT Energy Drink®. All energy drinks had buffering capacity values higher than Coca-Cola®. Analysing the eroded enamel surface, the specimens submitted to TNT Energy Drink® had the greatest percentage loss of surface microhardness, surface loss, depth, and mineral loss, followed by those submitted to Red Bull® and Monster Energy®. Surface loss was the only predictor of mineral loss (p<0.001). CONCLUSIONS: Based on the study model employed, all the energy drinks examined were erosive to tooth enamel and TNT Energy Drink® had the worst behaviour. Key words:Energy drinks, tooth erosion, tooth demineralisation, hardness tests, quantitative light-induced fluorescence.
RESUMO
AIM: The aim of this study was to investigate the smoking habit influence on DNA methylation status in the promoters of the cancer related-genes MLH1, hTERT and TP53 in oral epithelial cells of healthy subjects. MATERIALS AND METHODS: DNA methylation analysis was performed using methylation-sensitive restriction enzymes (MSRE) in oral epithelial cells from non-smokers, smokers and ex-smokers. RESULTS: The investigated CpG dinucleotides located at HhaI and HpaII sites in the MLH1 gene promoter were observed to be fully methylated in the majority of DNA samples from the smoker group and statistical differences were found between non-smokers and smokers and between smokers and ex-smokers (p<0.05). The same was observed in the hTERT gene promoter at HhaI sites (p<0.05) and for HpaII sites the unmethylated condition was more frequent in smokers in comparison to non-smokers (p<0.05). For TP53, no differences were found among groups (p>0.05), with the fully methylated condition found to be a common event in healthy oral epithelial cells. CONCLUSION: We conclude that smoking may induce changes in DNA methylation status in cancer-related genes of oral epithelial cells and that the cessation of smoking is capable of reversing this process. Based on our data, we suggest that DNA methylation status of the hTERT and MLH1 gene promoters are promising markers for screening a set of smoking-related alterations in oral cells.
Assuntos
Proteínas Adaptadoras de Transdução de Sinal/genética , Biomarcadores Tumorais/análise , Metilação de DNA , Células Epiteliais/patologia , Mucosa Bucal/citologia , Neoplasias Bucais/diagnóstico , Neoplasias Bucais/genética , Proteínas Nucleares/genética , Fumar , Proteína Supressora de Tumor p53/genética , Feminino , Marcadores Genéticos , Humanos , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/patologia , Proteína 1 Homóloga a MutL , Reação em Cadeia da Polimerase , Regiões Promotoras GenéticasRESUMO
O esmalte dentário é um tecido duro, submetido constantemente aos processos de des e remineralização pelo contato com os fluidos bucais. Acredita-se que é necessário compreender o mecanismo de formação do esmalte para entender como os ácidos orgânicos e inorgânicos interferem na sua estrutura por visão microscópica e clínica. Desta forma, o objetivo desse trabalho foi realizar uma revisão da literatura sobre os aspectos clínicos e histopatológicos de dois tipos de desgaste dentário: cárie e erosão. Enfatiza-se que apesar da cárie e da erosão serem resultado de processos de perda de tecido do dente, o mecanismo de ação do agente etiológico é diferenciado a nível microestrutural, repercutindo em aspectos clínicos distintos.
Dental enamel is a hard tissue, des and remineralization processes is frequently because buccal fluids constant expouse. It is believed that is necessary to understand the enamel formation mechanism to understand as the organic and inorganic acids interfere in structure one for microscopic and clinical vision. This way, the aim of this work was carry out literature review about clinical and histopatological aspects of two types of dental wear: caries and erosion. It is emphasized that dental caries and erosion resulted of loss tooth tissue processes, the etiological agent action mechanism is diff erentiated by microstrutural and clinical level.
RESUMO
O objetivo desse estudo foi avaliar o perfil profissional dos Odontopediatras e Dentistas Clínicos Gerais que atendem crianças na cidade de João Pessoa - PB, Brasil. A amostra foi constituída de dois grupos: 26 Odontopediatras e 56 Cirurgiões-dentistas (proporção aproximada de 1:2). Uma entrevista foi realizada para investigar os seguintes aspectos: faculdade de graduação, tempo de formado, local de trabalho e titulação profissional. Observou-se predominância do gênero feminino nos dois grupos, sendo 96,15% dos Odontopediatras e 91,1% dos Clínicos Gerais. Em relação ao tipo de faculdade, se pública ou privada, 96,15% dos Odontopediatras e 96,4% dos Clínicos Gerais fizeram a graduação em instituições públicas. Trabalharam a maior parte do tempo no setor privado, 65,38% dos Odontopediatras e 53,6% dos Clínicos Gerais. Apenas 23,08% dos Odontopediatras realizaram pós-graduação em nível de Mestrado e/ou Doutorado. Entre os Clínicos Gerais, um terço (32,1%) relatou ter realizado curso de pós-graduação. O profissional que atende crianças na cidade de João Pessoa - PB caracteriza-se por ser predominantemente do gênero feminino, ter formação em instituição pública de ensino e dedicar maior tempo de trabalho ao setor privado.
The aim of this study was to evaluate the pediatric dentistry and general dentistry profile of professionals working in the city of João Pessoa, Paraíba, Brazil. The sample was composed of two groups: 26 pediatric dentistry and 56 general dentistry (proportion of 1:2). The interview technique was used to investigate the following aspects: educational institution, professional years experience, work place and professional titulation. Among those interviewed, 96.15 and 91.1% of the pediatric dentistry and general dentistry, respectively, were of the feminine gender; 96.15 and 96.4% of the pediatric dentistry and general dentistry were graduated from public universities, respectively, and 65.38 and 53.6% of the pediatric dentistry and general dentistry, respectively, worked mostly in the private sector. Only 23.08% of pediatric dentistry professionals had attended to postgraduate school. About a third of the general dentistry (32.1%) had attended postgraduate school. Therefore, this data indicates that children living in Joao Pessoa, PB are attended by female practitioners, graduated from public universities, and who are largely employed on private sector.
Assuntos
Humanos , Autonomia Profissional , Odontólogos , Prática Profissional , Recursos Humanos em OdontologiaRESUMO
O objetivo desse estudo foi avaliar o perfil profissional dos Odontopediatras e Dentistas Clínicos Gerais que atendem crianças na cidade de João Pessoa - PB, Brasil. A amostra foi constituída de dois grupos: 26 Odontopediatras e 56 Cirurgiões-dentistas (proporção aproximada de 1:2). Uma entrevista foi realizada para investigar os seguintes aspectos: faculdade de graduação, tempo de formado, local de trabalho e titulação profissional. Observou-se predominância do gênero feminino nos dois grupos, sendo 96,15% dos Odontpediatras e 91,1% dos Clínicos Gerais. Em relação ao tipo de faculdade, se pública ou privada, 96,15% dos Odontopediatras e 96,4% dos Clínicos Gerais fizeram a graduação em instituições públicas. Trabalharam a maior parte do tempo no setor privado, 65,38% dos Odontopediatras e 53,6% dos Clínicos Gerais. Apenas 23,08% dos Odontopediatras realizaram pós-graduação em nível de Mestrado e/ou Doutorado. Entre os Clínicos Gerais, um terço (32,1%) relatou ter realizado curso de pós-graduação. O profissional que atende crianças na cidade de João Pessoa - PB caracteriza-se por ser predominantemente do gênero feminino, ter formação em instituição pública de ensino e dedicar maior tempo de trabalho ao setor privado.
The aim of this study was to evaluate the pediatric dentistry and general dentistry profile of professionals working in the city of João Pessoa, Paraíba, Brazil. The sample was composed of two groups: 26 pediatric dentistry and 56 general dentistry (proportion of 1:2). The interview technique was used to investigate the following aspects: educational institution, professional years experience, work place and professional titulation. Among those interviewed, 96.15 and 91.1% of the pediatric dentistry and general dentistry, respectively, were of the feminine gender; 96.15 and 96.4% of the pediatric dentistry and general dentistry were graduated from public universities, respectively, and 65.38 and 53.6% of the pediatric dentistry and general dentistry, respectively, worked mostly in the private sector. Only 23.08% of pediatric dentistry professionals had attended to postgraduate school. About a third of the general dentistry (32.1%) had attended postgraduate school. Therefore, this data indicates that children living in Joao Pessoa, PB are attended by female practitioners, graduated from public universities, and who are largely employed on private sector.
