Microencapsulation of roasted mate tea extractives with lasiodiplodan (a (1 → 6)-ß-D-glucan) and maltodextrin as combined coating materials: A strategic tool to stabilize and protect the bioactive components.

Microencapsulation has emerged as a promising strategy to enhance the stability and protection of bioactive compounds. In this work, roasted mate tea was microencapsulated using 15 % maltodextrin and lasiodiplodan (0.5-1.25 %) as wall coating materials. The microcapsules were characterized for encapsulation efficiency, hygroscopicity, moisture, water activity, water solubility, dissolubility, scanning electron microscopy, FT-IR spectroscopy, thermal analysis, colorimetry, antioxidant activity, as well as quantification of phenolic compounds and caffeine. Microencapsulation yields ranged from 44.92 to 56.39 %, and the efficiency of encapsulation varied from 66.54 to 70.16 by increasing the lasiodiplodan concentration. FT-IR revealed phenolic acids, flavonoids, and polyphenolics. Minor color variations were observed among the samples. Thermal analysis demonstrated the microencapsulates exhibited good thermal stability with no degradation below 250 °C. Encapsulated samples showed high levels of bioactive compounds, suggesting that microencapsulation by spray-drying was a favorable process, where maltodextrin, a low-cost protective agent, when combined with the properties of lasiodiplodan, can be a good option for stabilizing mate extracts.

Effects of an alternative bleaching method for yerba mate leaves on total phenolic content: evaluation and prediction with soft-computing approaches.

BACKGROUND: The effects of an alternative bleaching method on the total phenolic content (TPC) and antioxidant activity (AA) of yerba mate extracts were evaluated. Traditional bleaching ('zapeco') was compared with submerging the leaves in water followed by a hot air oven-drying process. Seven different approaches, i.e. linear model (LM), response surface model (RSM), Mamdani, Larsen, adaptive neuro-fuzzy inference system (ANFIS) with the product (Prod) and the minimum (Min) operators, and ANFIS with automatically membership functions (Auto), were employed to compare the TPC of yerba mate extracts based on drying temperature and AA assays. RESULTS: The results showed that if leaves were bleached followed by drying at higher temperatures, we obtained higher AA and TPC values. For submerging bleaching treatment, RSM model delivered the best accuracy measures with a mean absolute error (MAE), average absolute percentage error (MAPE), and mean squared error (MSE) of 0.128, 0.006, and 0.028, respectively. The ANFIS Auto model was the best for traditional bleaching treatment, with MAE, MAPE, and MSE of 0.490, 0.013, and 0.612, respectively. CONCLUSION: The results suggest a second-order linear relation between drying temperature, AA assays to TPC, and a high level of relation complexity of drying temperature, AA assays, and TPC. The evaluated soft-computing approaches have the excellent ability to estimate TPC from bleached leaves. © 2022 Society of Chemical Industry.

Hydrogel containing (1 → 6)-ß-D-glucan (lasiodiplodan) effectively promotes dermal wound healing.

A hydrogel containing exocellular (1 → 6)-ß-D-glucan (lasiodiplodan, LAS) was developed and its wound healing potential was evaluated. ß-Glucans have attracted much interest by the cosmetic industry sector because of their bioactive and functional properties and in promoting skin health. In the present work an ß-glucan was studied as a healing biomaterial that has not hitherto been reported in the scientific literature. LAS produced by the ascomycete Lasiodiplodia theobromae MMPI was used in the formulation of a healing hydrogel. Physicochemical and microbiological quality parameters, antioxidant potential and stability of the formulation was evaluated. FTIR, thermal analysis and SEM techniques were also employed in the characterization. Wistar rats were used as a biological model to investigate the wound healing potential. Histological analyses of cutaneous tissue from the dorsal region were conducted after 4, 7, 10 and 14 days of treatment, and evaluated re-epithelialization, cell proliferation and collagen production. Physicochemical stability, microbiological quality and antioxidant potential, especially in relation to its ability to scavenge hydroxyl radicals were found. The hydrogel stimulated cell re-epithelialization and proliferation during all days of the treatment, and stimulated an increase of collagen fibers. Lasiodiplodan showed immunomodulatory activity in wound healing and this biomacromolecule could be an alternative compound in wound care.

Amino acid profile of food fishes with potential to diversify fish farming activity.

In Brazil, 47% of the fish farming correspond to the tilapia species, and the main producers are the south and northeast regions. This work aimed at characterizing the amino acid profile of three fishes with the potential for rearing and diversification in Brazil and worldwide. The fishes grass carp (Ctenopharyngodon idella), pacu (Piaractus mesopotamicus), and catfish (Ictalurus punctatus) were obtained from a rural property located in the city of Pato Branco, PR, Brazil, and analyzed regarding the amino acid composition through High-Performance Liquid Chromatography. The amino acid profile showed the presence of glutamic acid, followed by lysine, aspartic acid, and leucine as the most prominent amino acids for the three fishes. Among the studied species, the grass carp presented the highest content of essential amino acids, registering all values superior to the minimum recommendation standards determined by the Food and Agriculture Organization of the United Nations (FAO). The disclosure of the significant nutritional value presented by these species consolidate their protein quality and expand possibilities to improve the fish farming and development of derivative products.

Proximate composition and kinectics drying of sweet pine nuts compared to typical nuts of Araucaria angustifolia / Composição proximal e cinética de secagem de pinhões doces e pinhões típicos de Araucaria angustifolia

ABSTRACT: The objective of this research was to determine the proximate composition and kinetics drying of sweet pine nuts compared to typical pine nuts of Araucaria angustifolia. This study is based on the proximate components, color, and duration of pine nuts drying of both types, and the influence of temperature and cutting geometry on the dehydration process. Sweet pine nuts had lower calorific value and carbohydrate content, but higher lipid, dietary fiber, protein, and ash contents when compared to typical pine nuts. Color of sweet pine nuts is light pink whereas the typical pine nut is yellowish-white. Sweet pine nuts were also softer. Drying kinetics of the seeds fit well into the logistic and Thompson models. To accelerate drying rates, we recommend slicing pine nuts into thin cross-sections and drying them at temperatures of 60 °C for typical pine nuts and 70 °C for sweet pine nuts.

RESUMO: O objetivo deste trabalho foi determinar a composição centesimal e a cinética de secagem de pinhões doces em comparação com pinhões típicos de Araucaria angustifolia. Este estudo baseia-se na composição centesimal, cor e duração da secagem dos pinhões de ambos os tipos e na influência da temperatura e da geometria de corte no processo de desidratação. Os pinhões doces apresentaram menor valor calorífico e conteúdo de carboidratos. Por outro lado, apresentaram maior teor de lipídios, fibras alimentares, proteínas e cinzas quando comparados aos pinhões típicos. A cor dos pinhões doces é rosa claro, enquanto o pinhão típico é branco amarelado. Os pinhões doces também são mais macios. A cinética de secagem das sementes se enquadra bem nos modelos logístico e de Thompson. Para acelerar as taxas de secagem, recomendamos fatiar os pinhões em seções finas e secá-los a temperaturas de 60 °C para pinhões típicos e 70 °C para pinhões doces.

Acceptance of Santo Giorno cheese typical of the Southwestern region of Paraná, Brazil / Aceitação do queijo Santo Giorno típico da região Sudoeste do Paraná

ABSTRACT: Santo Giorno cheese, obtained from raw milk and selected autochthonous starters, is emerging as the newest typical food product from the Southwestern region of Paraná, Brazil. The objective of this study was to evaluate the acceptance of the cheese with two ripening times of 60 and 180 days, produced in two dairy factories, testing two starters and two preservatives. Subjective sensory evaluation was applied using 129 consumers and hedonic scales for the attributes and for purchasing intent. A questionnaire involving the rate of cheese consumption was also used. The results suggested good reproducibility of the cheese preparation by the dairy factories, with no significant differences between the type of preservative used and the suitability of the two types of starter tested. An inverse relationship between hedonic scores for attributes (appearance, color, odor, texture, flavor) and ripening time was observed, except for texture. By applying multinomial logistic regression and a box plot analysis, a significant effect of age on cheese purchasing intent was verified, with a mean acceptance rate of 87.8% for 60 days ageing and 81.8% for 180 days ageing. Results revealed a possible Santo Giorno cheese public of older consumers who had a cheese consuming habit.

RESUMO: Obtido a partir de leite cru e fermento autóctone selecionado, o queijo Santo Giorno surge como o mais novo produto alimentício típico da Região Sudoeste do Paraná. O objetivo do presente trabalho foi avaliar a aceitação do queijo em dois tempos de maturação 60 e 180 dias produzidos em dois laticínios, onde foram testados dois fermentos desenvolvidos e dois conservantes. Avaliação sensorial subjetiva foi aplicada a um total de 129 consumidores através do uso de escalas hedônicas por atributos e intenção de compra. Questionário envolvendo a frequência de consumo de queijos também foi utilizado. Os resultados sugeriram reprodutibilidade na elaboração dos queijos pelos laticínios, diferenças não significativas no tipo de conservante a ser empregado e aptidão para os dois tipos de fermentos testados. Relação inversa entre os escores hedônicos dos atributos (aparência, cor, odor, sabor) e o tempo de maturação foram verificadas, exceção para o atributo textura. Mediante aplicação de regressão logística multinomial e análises por box plot verificou-se importante efeito da idade na intenção de compra do queijo. Com índice de aceitação médio de 87,8 % para 60 dias e 81,8 % para 180 dias, os resultados revelam um possível público do queijo Santo Giorno, consumidores com mais idade e que possuem o hábito de consumir queijos.

Milk coagulation properties and methods of detection / Propriedades de coagulação do leite e métodos de detecção

ABSTRACT: One of the most crucial steps in cheesemaking is the coagulation process, and knowledge of the parameters involved in the clotting process plays an important technological role in the dairy industry. Milk of different ruminant species vary in terms of their coagulation capacities because they are influenced by the milk composition and mainly by the milk protein genetic variants. The milk coagulation capacity can be measured by means of mechanical and/or optical devices, such as Lactodynamographic Analysis and Near-Infrared and Mid-Infrared Spectroscopy.

RESUMO: Uma das etapas mais importantes na fabricação de queijos é o processo de coagulação. O conhecimento dos parâmetros envolvidos nesse processo desempenha um papel tecnológico importante para a indústria de laticínios. O leite de diferentes espécies de ruminantes varia em termos de capacidade de coagulação, pois são influenciados pela composição e principalmente pelas variantes genéticas da caseína. Estas características podem ser avaliadas por meio de dispositivos mecânicos e/ou ópticos, tais como, a Análise Lactodinamográfica e a Espectroscopia de Reflectância do Visível ao Infravermelho Próximo (NIR) e Médio (MIR).