Assembling the layers of the hematopoietic system: A window of opportunity for thymopoiesis in the embryo.

During embryonic development, several independent generations of hematopoietic cells were identified. They occur in the yolk sac and the intra-embryonic major arteries, in a narrow window of development. They arise sequentially, starting with primitive erythrocytes in the yolk sac blood islands, progressing to less differentiated erythromyeloid progenitors still in the yolk sac, and culminating with multipotent progenitors, some of which will generate the adult hematopoietic stem cell compartment. All these cells contribute to the formation of a layered hematopoietic system that reflects adaptative strategies to the fetal environment and the embryo's needs. It is mostly composed, at these stages, of erythrocytes and tissue-resident macrophages both of yolk sac origin, the latter persisting throughout life. We propose that subsets of lymphocytes of embryonic origin derive from a different intra-embryonic generation of multipotent cells occurring before the emergence of hematopoietic stem cell progenitors. These multipotent cells have a limited lifespan and generate cells that provide basic protection against pathogens before the adaptive immune system is functional, contribute to tissue development and homeostasis, and shape the establishment of a functional thymus. Understanding the properties of these cells will impact the understanding of childhood leukemia and of adult autoimmune pathology and thymic involution.

Isolated open tibial shaft fracture: a seven-hospital, prospective observational study in two Latin America countries.

INTRODUCTION: open tibial fractures are challenging due to the frequent severe bone injury associated with poor soft tissue conditions. This is relevant in low- and middle-income countries, mainly related to delayed definitive fixation and lack of adequate training in soft tissue coverage procedures. Due to these factors, open tibial fracture is an important source of disability for Latin American countries. Herein we sought to provide an epidemiological overview of isolated open tibial shaft fracture across seven hospitals in southern cone of Latin America. The secondary goal was to assess the impact on quality of life based on return-to-work rate (RWR). METHODS: patients with an isolated open tibial shaft fracture treated in seven different hospitals from Brazil and Argentina from November 2017 to March 2020 were included in the study. Clinical and radiographic results were evaluated throughout the 120-day follow-up period. Final evaluation compared RWR with the SF-12 questionnaire, bone healing, and gait status. RESULTS: Seventy-two patients were treated, 57 followed for 120 days and 48 completed the SF-12 questionnaire. After 120 days, 70.6% had returned to work, 61.4% had experienced bone healing. Age, antibiotic therapy, type of definitive treatment, and infection significantly influenced the RWR. Gait status exhibited strong correlations with RWR and SF-12 physical component score. CONCLUSIONS: Isolated open tibial shaft fractures are potentially harmful to the patient's quality of life after 120 days of the initial management. RWR is significantly higher for younger patients, no history of infection, and those who could run in the gait status assessment.

An Alternative Study about the Geometry and the First Law of Thermodynamics for AdS Lovelock Gravity, Using the Definition of Conserved Charges.

In this work, we introduce an extension of the study of the first law of thermodynamics of black holes based on the geometry of the extended phase space for AdS Lovelock gravities, which includes changes in scales. As expected, the result obtained coincides with the previously known four-dimensional case. For higher dimensions, the result is the rise of two new contributions to the first law of thermodynamics. The first term corresponds to corrections of the usual definition of thermodynamic volumes at the horizon due to the presence of the higher curvature terms. The second term arises in odd dimensions, comes from the asymptotic region, and corresponds to a scale transformation of the form ∝δ^ln(l/ℓ), with l the AdS radius and ℓ a parameter. A particularly interesting case corresponds to the Chern Simons gravity where the change scale does not generate a contribution at the asymptotic region, likely due to the Chern Simons AdS local symmetry.

Patellofemoral Pain Syndrome Risk Associated with Squats: A Systematic Review.

Patellofemoral pain syndrome (PFPS) is highly prevalent; it can cause severe pain and evolve into progressive functional loss, leading to difficulties performing daily tasks such as climbing and descending stairs and squatting. This systematic review aimed to find evidence, in the literature, of squat movements that can cause or worsen PFPS. This work was based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement, and its protocol was registered in PROSPERO (CRD42019128711). From the 6570 collected records, 37 were included. From these 37 articles, 27 present a causal relationship between knee flexion and PFPS, 8 describe a relationship, considering the greater existence of muscle contractions, and one article did not describe this relationship in its results. The main limitations stem from the fact that different studies used different evaluation parameters to compare the force exerted on the patellofemoral joint. Furthermore, most studies are focused on sports populations. After analysing the included works, it was concluded that all squat exercises can cause tension overload in the knee, especially with a knee flexion between 60° and 90° degrees. The main causal/worsening factors of PFPS symptoms are the knee translocation forward the toes (on the same body side) when flexing the knee, and the muscle imbalance between the thigh muscles.

Epidemiology of COVID-19 Among Indigenous Populations in Brazil.

BACKGROUND: Due to social and geographical isolation, indigenous people are more vulnerable to adverse conditions; however, there is a lack of data on the epidemics' impact on these populations. Thus, this article's objective was to describe the epidemiological situation of COVID-19 in indigenous communities in Brazil. METHODS: This descriptive observational study was carried out in indigenous communities in the municipality of Amaturá (Amazonas, Brazil). Individuals from the Alto Rio Solimões Special Indigenous Sanitary District (DSEI) who met the Sars-Cov-2 infection case definitions during the period between January and August 2020 were included. For case notification, the definitions adopted by the Ministry of Health of Brazil and by the Special Secretariat for Indigenous Health were considered. RESULTS: Out of the entire population served by the Alto Rio Solimões DSEI (n = 2890), 109 indigenous people were suspected of having been infected with Sars-Cov-R during the study period; a total of 89 cases were actually confirmed (rate: 3.08 cases/100,000 inhabitants). Most patients diagnosed with COVID-19 were female (56.2%), with a mean age of 32.4 (± 23.6) years. Predominant symptoms were fever (76.4%), dry cough (64%), and headache (60.7%). Complications occurred in 7.9% of the patients; no deaths were reported. CONCLUSION: These results enhance the observation that indigenous populations, even if relatively isolated, are exposed to COVID-19. The disease cases assessed showed a favorable evolution, which does not mean reducing the need for caring of this population.

Isolated open tibial shaft fracture: a seven-hospital, prospective observational study in two Latin America countries / Fraturas expostas isoladas da diáfise da tíbia: estudo prospectivo observacional em sete hospitais de dois países da América Latina

ABSTRACT Introduction: open tibial fractures are challenging due to the frequent severe bone injury associated with poor soft tissue conditions. This is relevant in low- and middle-income countries, mainly related to delayed definitive fixation and lack of adequate training in soft tissue coverage procedures. Due to these factors, open tibial fracture is an important source of disability for Latin American countries. Herein we sought to provide an epidemiological overview of isolated open tibial shaft fracture across seven hospitals in southern cone of Latin America. The secondary goal was to assess the impact on quality of life based on return-to-work rate (RWR). Methods: patients with an isolated open tibial shaft fracture treated in seven different hospitals from Brazil and Argentina from November 2017 to March 2020 were included in the study. Clinical and radiographic results were evaluated throughout the 120-day follow-up period. Final evaluation compared RWR with the SF-12 questionnaire, bone healing, and gait status. Results: Seventy-two patients were treated, 57 followed for 120 days and 48 completed the SF-12 questionnaire. After 120 days, 70.6% had returned to work, 61.4% had experienced bone healing. Age, antibiotic therapy, type of definitive treatment, and infection significantly influenced the RWR. Gait status exhibited strong correlations with RWR and SF-12 physical component score. Conclusions: Isolated open tibial shaft fractures are potentially harmful to the patient's quality of life after 120 days of the initial management. RWR is significantly higher for younger patients, no history of infection, and those who could run in the gait status assessment.

RESUMO Introdução: o tratamento de fraturas expostas isoladas da diáfise da tíbia (FEIDT) apresenta desafios por frequentemente associar severa lesão óssea com condições ruins de tecido mole, fatores relevantes em países de média e baixa renda, especialmente devido a atrasos na implementação da fixação definitiva e falta de treinamento adequado no manejo de tecidos moles. Consequentemente, FEIDTs representam importante fonte de incapacitação na América Latina. Este estudo objetivou apresentar uma visão geral das FEIDTs em sete hospitais do cone sul da América Latina. O objetivo secundário foi avaliar o seu impacto na qualidade de vida baseado na taxa de retorno ao trabalho (TRT). Métodos: foram incluídos no estudo pacientes com FEIDT tratados em sete hospitais de Brasil e Argentina entre novembro de 2017 e março de 2020. Resultados clínicos e radiográficos foram analisados num período de 120 dias. Avaliação final comparou TRT com o questionário SF-12, consolidação óssea e condições de marcha. Resultados: setenta e dois pacientes foram tratados, 57 seguidos por 120 dias e 48 completaram o questionário SF-12. Após 120 dias, 70,6% havia retornado ao trabalho, 61,4% tinha fratura consolidada. Idade, antibioticoterapia, tipo de tratamento definitivo e infecção influenciaram significativamente na TRT. A condição de marcha apresentou forte correlação com TRT e o componente físico do SF-12. Conclusão: FEIDTs são potencialmente deletérias à qualidade de vida dos pacientes 120 dias após o tratamento inicial. TRT é significativamente maior para pacientes mais jovens, sem história de infecção e que conseguem correr na avaliação da condição de marcha..

Sexually transmitted infections in indigenous communities of the Alto Rio Solimões / Infecções sexualmente transmissíveis em comunidades indígenas do Alto Rio Solimões

Introduction: The prevalence of sexually transmitted infections (STI) among indigenous communities is an appalling issue related to Brazilian public health, as there is an increasing underreporting and neglect related to the study and care of these people. Objective: To determine the prevalence of STI in the indigenous population of the Alto Rio Solimões. Methods: STI diagnostic records from the database of the Indigenous Health Care Information System - SIASI, of the indigenous communities of the Alto Rio Solimões, belonging to the Nova Itália base, in Amazonas, were evaluated during the period from January 2010 to August 2020. Sociodemographic data were also evaluated to determine the profile of the diagnosed indigenous population and the geographical and temporal distribution of cases. Results: The overall prevalence rate of STIs was 3.91% (113 notifications of STI in the population of 2890 indigenous people). The largest number of diagnosed cases was in Nova Itália (60.17%). The ethnic group with the highest number of cases was Tikuna (92.03%). Among the STI studied, gonorrhea / chlamydia had the highest prevalence (68.14%), followed by Hepatitis B (13.27%) and Syphilis (10.61%). Most cases were found among women (71.7%), aged 30­34 years. Conclusion: A higher prevalence of STIs was observed in indigenous women, mainly from the Nova Itália town and the Tikuna ethnic group.

Introdução: A prevalência das infecções sexualmente transmissíveis (IST) entre comunidades indígenas é um tema consternador relacionado à saúde pública brasileira, pois há crescente subnotificação e negligência relacionada ao estudo e ao cuidado desses povos. Objetivo: Determinar a prevalência de IST na população indígena do Alto Rio Solimões. Métodos: Foram avaliados os registros diagnósticos de IST da base de dados do Sistema de Informação da Atenção à Saúde Indígena (SIASI), das comunidades indígenas do Alto Rio Solimões, pertencentes ao polo-base de Nova Itália, no Amazonas, durante o período de janeiro de 2010 a agosto de 2020. Também foram avaliados dados sociodemográficos para determinação do perfil da população indígena diagnosticada e a distribuição geográfica e temporal dos casos. Resultados: A taxa de prevalência geral de IST foi de 3,91% (113 notificações de IST na população de 2.890 indígenas). O maior número de casos diagnosticados foi em Nova Itália (60,17%). A etnia com maiores números de casos foi a Tikuna (92,03%). Entre as IST estudadas, gonorreia/clamídia tiveram a maior prevalência (68,14%), seguidas por hepatite B (13,27%) e sífilis (10,61%). A maioria dos casos ocorreu entre mulheres (71,7%) e na faixa de 30­34 anos. Conclusão: Observou-se maior prevalência de IST em mulheres indígenas, principalmente do município de Nova Itália e da etnia Tikuna

Entendimento das políticas públicas de medicamentos e assistência farmacêutica por estudantes de medicina / Understanding of public policies for medicines and pharmaceutical assistance by medical students

Introdução: A Política Nacional de Medicamentos e a Política Nacional de Assistência Farmacêutica devem ser compreendidas como norteadoras para a formulação de políticas setoriais. Este estudo teve como objetivo analisar a compreensão das Políticas Públicas de medicamentos no Sistema Único de Saúde por estudantes de medicina de Santa Catarina. Métodos: Trata-se de estudo descritivo, transversal, com abordagem quantitativa, que utilizou como instrumento para a coleta de dados um questionário autoaplicável, semiestruturado, composto por 16 questões. Tal questionário foi adaptado a partir da Pesquisa Nacional sobre Acesso, Utilização e Promoção do Uso Racional de Medicamentos - PNAUM. A população de estudo foram 30 acadêmicos do curso de medicina. Foram utilizadas ferramentas de análise estatística descritiva, por meio da determinação de média, desvio padrão, frequência absoluta e relativa. Resultados: Os resultados demonstraram fragilidade no tocante à compreensão das políticas públicas de medicamentos pelos participantes, sendo entendida de uma forma reducionista como a simples disponibilização de medicamentos pelo governo, em sua maioria. Conclusão: Faltam ainda, entendimentos sobre as formas de seleção e acesso a medicamentos, bem como da sua importância para a garantia da efetividade das ações de assistência farmacêutica no SUS.

Introduction: The National Medicines Policy and the National Pharmaceutical Assistance Policy should be understood as guidelines for the formulation of sectoral policies. This study aimed to analyze the understanding of Public Policies on medicines in the Unified Health System (SUS) by medical students in Santa Catarina. Methods: This is a descriptive, cross-sectional study with a quantitative approach, which used a self-administered, semi-structured 16-question questionnaire as an instrument for data collection. This questionnaire was adapted from the National Survey on Access, Use and Promotion of Rational Use of Medicines (PNAUM). The study population was 30 medical students. Descriptive statistical analysis tools were used, through the determination of mean, standard deviation, and absolute and relative frequency. Results: The results showed fragility regarding the understanding of public policies for medicines by the participants, being understood for the most part in a reductionist way as the simple provision of medicines by the government. Conclusion: There is still a lack of understanding about the forms of selection and access to medicines, as well as their importance to guarantee the effectiveness of pharmaceutical assistance actions in the SUS.

Depressão em dependentes químicos: uma revisão sistemática da literatura / Depression in drug addicts: a systematic literature review

RESUMO Introdução: A co-ocorrência entre o abuso de substâncias químicas e o transtorno depressivo é reconhecida cada vez mais como um problema de saúde pública em âmbito mundial. Objetivos: compreender a problemática da depressão no dependente químico segundo uma análise da literatura nacional e internacional. Método: realizou-se uma revisão de artigos científicos publicados nas seguintes plataformas de pesquisa: Portal de Periódicos Eletrônicos de Psicologia (PePSIC), Cochrane Collaboration e Scientific Electronic Library Online (SCIELO). Resultados: foram encontrados 83 estudos relacionados aos descritores utilizados, sendo que após a primeira e segunda análise de adequação aos objetivos da revisão restaram oito. Os resultados evidenciaram que a prevalência de transtornos depressivos nos dependentes químicos teve uma média de 21,27%. O método diagnóstico utilizado com maior frequência foi o Inventário de Depressão de Beck (30%). Conclusões: Constatou-se que a prevalência de transtornos depressivos em dependentes químicos é de aproximadamente três vezes o da população em geral. Verificou-se que as "avaliações rápidas" como os instrumentos MINI e Inventário de depressão de Beck foram os mais utilizados para realização do diagnóstico de depressão na pessoa com dependência química. PALAVRA-CHAVE: Depressão, dependência química, alcoolismo

ABSTRACT Introduction: The co-occurrence between substance abuse and depressive disorder is increasingly recognized as a public health problem worldwide. Objectives: To understand the problem of depression in drug addicts according to an analysis of national and international literature. Method: A review of scientific articles published in the following research platforms was carried out: Psychology Electronic Journal Portal (PePSIC), Cochrane Collaboration, and Scientific Electronic Library Online (SCIELO). Results: Eighty-three studies related to the keywords used were found, and after the first and second analysis of adequacy to the review objectives, eight remained. The results showed that the prevalence of depressive disorders in drug addicts had an average of 21.27%. The most frequently used diagnostic method was the Beck Depression Inventory (30%). Conclusions: It was found that the prevalence of depressive disorders in drug addicts is approximately three times that of the general population. It was found that "quick assessments" such as the MINI and the Beck Depression Inventory were the most used to diagnose depression in people with drug addiction. KEYWORDS: Depression, drug addiction, alcoholism

O suicídio em indígenas da Amazônia Brasileira: revisão sistemática da literatura / Suicide in indigenous peoples in the Brazilian Amazon: a systematic literature review

RESUMO Introdução: No Brasil, o suicídio não atinge apenas as populações urbanas, mas se estende aos rincões culturais mais longínquos do país, como as populações quilombolas, ribeirinhas, do campo e indígenas das mais diversas etnias. O objetivo deste artigo foi apresentar dados sobre a mortalidade e os fatores associados ao suicídio em indígenas da região amazônica brasileira. Métodos: Foi realizada revisão sistemática da literatura, em bases de dados eletrônicas em artigos publicados entre 2000 e 2019. Resultados: Os resultados mostraram que a prevalência do suicídio entre indígenas é de aproximadamente 22,83%, e atinge principalmente homens (73,3%), jovens (47,7%), solteiros (79.5%) e que utilizam bebidas alcoólicas. Também observou-se que o local escolhido de preferência para a prática do suicídio é a própria residência (80,3%) e o enforcamento o método mais utilizado (85,5%). Conclusão: A população indígena apresenta prevalência de suicídio quatro vezes maior do que a população residente em área urbana. Sugere-se a realização de investigações sociais in loco nas aldeias como meio de obter maior compreensão sobre o fenômeno suicídio em indígenas. PALAVRA-CHAVE: indígenas, suicídio, amazônia.

ABSTRACT Introduction: In Brazil, suicide does not only affect urban populations, but extends to the most distant cultural corners of the country, such as quilombola, riverside, rural and indigenous populations of the most diverse ethnic groups. The aim of this article was to present data on mortality and factors associated with suicide in indigenous peoples in the Brazilian Amazon region. Methods: A systematic literature review was carried out in electronic databases of articles published between 2000 and 2019. Results: The results showed that the prevalence of suicide among indigenous people is approximately 22.83%, and affects mainly men (73.3 %), young people (47.7%), single (79.5%) and who use alcoholic beverages. It was also observed that the preferred location for committing suicide is the residence itself (80.3%), and hanging the most used method (85.5%). Conclusion: The indigenous population has a 4-fold higher suicide prevalence than the population residing in urban areas. It is suggested that social investigations be carried out in the villages as a means to better understand the phenomenon of suicide in indigenous peoples. KEYWORDS: indigenous, suicide, amazon.

Musculoskeletal Disorders' Classification Proposal for Application in Occupational Medicine.

Occupational-specific classifications of musculoskeletal disorders (MSD) are scarce and do not answer specific clinical questions. Thus, a specific classification was developed and proposed, covering criteria applicable to daily clinical activity. It was considered that the disorder development process is the same across all work-related MSDs (WRMSDs). Concepts of clinical pathology were applied to the characteristics of WRMSDs pathophysiology, cellular and tissue alterations. Then, the correlation of the inflammatory mechanisms with the injury onset mode was graded into four levels (MSDs 0-3). Criteria of legal, occupational and internal medicine, semiology, physiology and orthopaedics, image medicine and diagnostics were applied. Next, the classification was analysed by experts, two occupational physicians, two physiatrists and occupational physicians and one orthopaedist. This approach will allow WRMSD prevention and improve therapeutic management, preventing injuries from becoming chronic and facilitating communication between occupational health physicians and the other specialities. The four levels tool relate aetiopathogenic, clinical, occupational and radiological concepts into a single classification. This allows for improving the ability to determine a WRMSD and understanding what preventive and therapeutic measures should be taken, avoiding chronicity. The developed tool is straightforward, easy to understand and suitable for WRMSDs, facilitating communication between occupational physicians and physicians from other specialities.

Prevalência do transtorno depressivo maior em pessoas com dependência química / Prevalence of major depressive disorder in people with chemical addiction / Prevalencia del trastorno depressive major en personas con adicción

OBJETIVO: verificar a prevalência do Transtorno Depressivo Maior em pessoas com dependência química. MÉTODO: estudo transversal com a aplicação da entrevista estruturada para os transtornos do DSM-V em 183 indivíduos internados em comunidades terapêuticas de acolhimento para dependentes químicos, localizadas no sul de Santa Catarina, durante o de ano de 2019. RESULTADOS: todos os participantes não realizaram consulta psiquiátrica ao ingressar na instituição e a maioria não realizou consulta psiquiátrica regular (94,5%). Constatou-se que 89 participantes (55,3%) possuíam critérios diagnósticos para o Transtorno Depressivo Maior e 59,1% destes possuíam adicção ao álcool (p<0,028). CONCLUSÃO: o diagnóstico do transtorno depressivo deve-se consolidar como uma variável importante para a eficácia do tratamento, visto que sua prevalência é elevada e possui repercussões sobre a qualidade do tratamento e tempo de institucionalização.

OBJECTIVE: to verify the prevalence of Major Depressive Disorder in people with chemical dependence. METHOD: cross-sectional study with the application of the structured interview for the DSM-V disorders in 183 individuals admitted to therapeutic communities for chemical dependents, located in the south of Santa Catarina, during the year 2019. RESULTS: All participants did not have a psychiatric consultation when entering the institution and the majority did not have a regular psychiatric consultation (94.5%). It was found that 89 participants (55.3%) had diagnostic criteria for Major Depressive Disorder and 59.1% of these had alcohol addiction (p<0.028). CONCLUSION: the diagnosis of depressive disorder should be consolidated as an important variable for the effectiveness of treatment, since its prevalence is high and has repercussions on the quality of treatment and time of institutionalization.

OBJECTIVO: verificar la prevalencia del transtorno depressivo major en personas con adicción. METODO: estudio transversal con aplicación de la entrevista estructurada para los transtornos del DSM-V en 183 personas internadas en comunides de tratamiento de adicción en el departamento de Santa Catarina durante el ano de 2019. RESULTADOS: la totalidad de los pacientes no realizaron cita con psiquiatra al ingressar en las instituiciones y la mayoria no realiza visitas regulares al psiquiatra (94,5%). Se observó que 89 participantes (55,3%) tenian critérios diagnósticos para depressión y 59,1% de ellos eram adictos al álcohol (p<0,028). CONCLUSIÓN: el diagnóstico del transtorno depressivo debe consolidarse como una variable importante para la eficácia del tratamiento, pues su prevalencia es elevada y pose repercursiones sobre la calidad del tratamiento y el tiempo de permanencia en las comunidades.

A wave of bipotent T/ILC-restricted progenitors shapes the embryonic thymus microenvironment in a time-dependent manner.

During embryonic development, multiple waves of hematopoietic progenitors with distinct lineage potential are differentially regulated in time and space. Two different waves of thymic progenitors colonize the fetal thymus where they contribute to thymic organogenesis and homeostasis. The origin, the lineage differentiation potential of the first wave, and their relative contribution in shaping the thymus architecture, remained, however, unclear. Here, we show that the first wave of thymic progenitors comprises a unique population of bipotent T and innatel lymphoid cells (T/ILC), generating a lymphoid tissue inducer cells (LTi's), in addition to invariant Vγ5+ T cells. Transcriptional analysis revealed that innate lymphoid gene signatures and, more precisely, the LTi-associated transcripts were expressed in the first, but not in the second, wave of thymic progenitors. Depletion of early thymic progenitors in a temporally controlled manner showed that the progeny of the first wave is indispensable for the differentiation of autoimmune regulator-expressing medullary thymic epithelial cells (mTECs). We further show that these progenitors are of strict hematopoietic stem cell origin, despite the overlap between lymphopoiesis initiation and the transient expression of lymphoid-associated transcripts in yolk sac (YS) erythromyeloid-restricted precursors. Our work highlights the relevance of the developmental timing on the emergence of different lymphoid subsets, required for the establishment of a functionally diverse immune system.

Mothers' competence profiles and their relation to language and socioemotional development in Chilean children at 12 and 30 months.

The study of early parental competences is relevant because such competences are related to children's development; however, most studies have considered competences using a variable-centered approach in which each parental competence is examined in isolation. This paper approaches these competences using a person-centered approach, generating profiles that combine different competences in Chilean mothers assessed when their children were aged 12 months and again at 30 months. The aim of this study was to generate and compare these profiles and to analyze the associations of these profiles with children's language and socioemotional skills. Mother-child interactions in the contexts of storytelling and free play were videotaped at two different times. Ninety mother-child dyads were assessed using the Adult Sensitivity Scale (E.S.A.), the Evaluation of the Mentalization of Significant Caregivers, the Checklist of Observations Linked to Outcomes (PICCOLO), Bayley's language scale and the Functional Emotional Assessment Scale (FEAS). Profiles of mothers' behaviors were identified through person-centered within-group analyses of six aspects: sensitivity, mentalization, affection, responsiveness, encouragement, and teaching. Cluster analyses yielded three similar profiles for mothers at both ages: highly competent, average competent, and poorly competent. The mothers' profiles were related to maternal age, socioeconomic status (SES) and educational level, and the mothers improved their profiles at the 30-month assessment. The mothers' profiles were related to children's language and socioemotional outcomes at both ages. These results and their applicability to promotion and intervention programs are discussed.

Hematopoiesis: A Layered Organization Across Chordate Species.

The identification of distinct waves of progenitors during development, each corresponding to a specific time, space, and function, provided the basis for the concept of a "layered" organization in development. The concept of a layered hematopoiesis was established by classical embryology studies in birds and amphibians. Recent progress in generating reliable lineage tracing models together with transcriptional and proteomic analyses in single cells revealed that, also in mammals, the hematopoietic system evolves in successive waves of progenitors with distinct properties and fate. During embryogenesis, sequential waves of hematopoietic progenitors emerge at different anatomic sites, generating specific cell types with distinct functions and tissue homing capacities. The first progenitors originate in the yolk sac before the emergence of hematopoietic stem cells, some giving rise to progenies that persist throughout life. Hematopoietic stem cell-derived cells that protect organisms against environmental pathogens follow the same sequential strategy, with subsets of lymphoid cells being only produced during embryonic development. Growing evidence indicates that fetal immune cells contribute to the proper development of the organs they seed and later ensure life-long tissue homeostasis and immune protection. They include macrophages, mast cells, some γδ T cells, B-1 B cells, and innate lymphoid cells, which have "non-redundant" functions, and early perturbations in their development or function affect immunity in the adult. These observations challenged the view that all hematopoietic cells found in the adult result from constant and monotonous production from bone marrow-resident hematopoietic stem cells. In this review, we evaluate evidence for a layered hematopoietic system across species. We discuss mechanisms and selective pressures leading to the temporal generation of different cell types. We elaborate on the consequences of disturbing fetal immune cells on tissue homeostasis and immune development later in life.

Medicina Integrativa: um parecer situacional a partir da percepção de médicos no Sul do Brasil / Integrative Medicine: situational assessment through medical perception in the South of Brazil

RESUMO O estudo teve por objetivo identificar o interesse, a concordância e o conhecimento dos médicos atuantes no Sistema Único de Saúde (SUS) no município de Tubarão (SC) em relação às práticas da Medicina Integrativa e sua política nacional de inclusão ao sistema de saúde. Foi realizado estudo transversal, em amostra do tipo censo, composta por um total de 230 profissionais. A maioria dos médicos investigados referiu desconhecer a Política Nacional de Práticas Integrativas e Complementares (85,5%) e negou possuir interesse pelas suas modalidades terapêuticas (53,3%). Ainda assim, um número significativo deles referiu querer conhecer mais sobre as práticas (43%) e concordar com a inclusão delas no SUS (80,2%), sendo que 48,8% concordam somente com a inclusão de algumas práticas. O uso próprio ou de familiares influenciou no conhecimento e, com isso, na recomendação de uso aos pacientes.

ABSTRACT The study aimed to identify the interest, agreement and knowledge of the doctors working in the Unified Health System (SUS) in the city of Tubarão (SC) regarding the practices of Integrative Medicine and their national policy of inclusion in the health system. A cross-sectional study was conducted on a census-type sample composed of a total of 230 professionals. Most investigated doctors reported not knowing the national policy of integrative and complementary practices (85.5%) and denied having interest in their therapeutic modalities (53.3%). Even so, a significant number of them reported wanting to know more about the practices (43%) and agreeing to include them in the SUS (80.2%), and 48.8% agree only with the inclusion of some practices. The use of one's own or family members influenced the knowledge and, therefore, the recommendation of use to the patients.

New Molecular Insights into Immune Cell Development.

During development innate lymphoid cells and specialized lymphocyte subsets colonize peripheral tissues, where they contribute to organogenesis and later constitute the first line of protection while maintaining tissue homeostasis. A few of these subsets are produced only during embryonic development and remain in the tissues throughout life. They are generated through a unique developmental program initiated in lympho-myeloid-primed progenitors, which lose myeloid and B cell potential. They either differentiate into innate lymphoid cells or migrate to the thymus to give rise to embryonic T cell receptor-invariant T cells. At later developmental stages, adaptive T lymphocytes are derived from lympho-myeloid progenitors that colonize the thymus, while lymphoid progenitors become specialized in the production of B cells. This sequence of events highlights the requirement for stratification in the establishment of immune functions that determine efficient seeding of peripheral tissues by a limited number of cells.

Liga de Anatomia Aplicada (LAA): as Múltiplas Perspectivas sobre Participar de uma Liga Acadêmica / League of Applied Anatomy (LAA): Multiple Perspectives on Participation in an Academic League

RESUMO Relata-se a experiência dos membros da Liga Acadêmica de Anatomia Aplicada (LAA) da Universidade do Estado do Rio de Janeiro (Uerj), sejam eles ligantes, gestores ou orientadores. A motivação para esse trabalho reside no desejo de apontar a importância dessas organizações estudantis e seu impacto sobre os alunos, considerando o recente crescimento e criação de diversas ligas. O modelo tradicional de ensino praticado pela maioria das academias no Brasil, caracterizado por uma relação vertical entre professores e alunos, justifica, ao menos em parte, a limitação criativa dos alunos, e consequentemente, dos futuros profissionais. É nesse panorama que as ligas acadêmicas são necessárias, buscando aguçar o espírito crítico dos alunos e tornar palpáveis os assuntos contemplados pela grade curricular tradicional, que, devido aos cronogramas e horários extenuantes, permanecem abstratos e pouco atrativos, contribuindo para a desmotivação e quebra de expectativa dos alunos ingressantes. Nesse sentido, também se busca atender às demandas dos novos acadêmicos, ansiosos pela aplicação prática do conteúdo abordado no ciclo básico. Todas as atividades precisam ser bem organizadas para que atinjam seus objetivos. Assim, os orientadores devem garantir a supervisão, os gestores devem ser bem selecionados, e a divisão de tarefas deve ser efetiva, de modo a conquistar o engajamento dos ligantes. Todo esse trabalho, seja para a produção de conteúdos científicos, seja para a composição de palestras, cursos ou simpósios, influencia diretamente o modo de pensar de cada parte envolvida. Junto a todo o processo descrito, a base para desenvolver as atividades consiste no tripé ensino-pesquisa-extensão, que complementa a aprendizagem, desenvolve a investigação e contribui para a disseminação dos saberes acadêmicos e o cumprimento do dever social. Neste trabalho, é possível entender o impacto que uma liga tem na formação dos estudantes de Medicina e no constante estímulo à atualização do docente. Assim, foram recolhidos depoimentos e aplicados questionários que ratificam a importância da liga no crescimento tanto pessoal, quanto profissional, nas diversas esferas, e como essas atividades extracurriculares têm se mostrado benéficas na aquisição de novas experiências para discentes e docentes.

ABSTRACT This article reports on the experience of members of the Academic League of Applied Anatomy, in Portuguese "Liga de Anatomia Aplicada (LAA)" of Universidade do Estado do Rio de Janeiro (Uerj), whether listeners, organizers or advisers. The motivation behind this article consists in the desire to address the importance of academic organizations and their impact on students, considering the recent growth and inauguration of various leagues. The traditional model of education, practiced in the majority of Brazilian, is characterized by a vertical relationship between the teacher and the students. This view partly the creative limitation imposed on the students and on future professionals. Therefore, Academic Leagues are necessary, as a means of improving, students' critical thinking and solidifying a subject that has already been taught in the curricula but that, due to exhausting schedules and rigid time frames, remains abstract and unappealing to the students, resulting in a lack of motivation and disappointed expectations. The league seeks to meet the demands of recently enrolled students, eager to apply in practice subjects still only seen in theory during the basic cycle of school. However, all the activities need to be well organized if they are to achieve their goals. Teachers must ensure supervision, the league's board of directors must be well-selected, and the division of tasks must be effective, in order to ensure the engagement of all the participants. All this work, whether for the production of scientific content, planning of lectures, courses or symposia, directly impacts the thought processes of all those involved. Alongside the whole process described, the basis of all this evolution is the teaching, research and extension activities, which complement the learning; develop research, and contribute to the dissemination of academic knowledge and the fulfillment of social duty. This paper shows the impact that a league has on the formation of medical students and on the constant encouragement for teachers to update their skills. Therefore, statements were collected and questionnaires conducted, to confirm the importance of the League for personal and professional growth in various spheres, and show how these extracurricular activities have proven to be beneficial in enabling students and teachers to acquire new experiences.

Factors underlying compliance with a healthy snacking initiative in the school environment: accounts of school principals in Montevideo (Uruguay).

OBJECTIVE: To evaluate the implementation of the Uruguayan healthy snacking initiative in primary and secondary schools in the capital, and to explore the factors underlying compliance from the perspective of school principals. DESIGN: A mixed-method approach was used, which included semi-structured interviews with school principals and a survey of the foods and beverages sold and advertised in the schools. SETTING: Primary and secondary schools in Montevideo (the capital city of Uruguay). PARTICIPANTS: School principals. RESULTS: The great majority of the schools did not comply with the initiative. Exhibition of non-recommended products was the main cause for non-compliance, followed by advertising of non-recommended products through promotional activities of food and beverage companies. Although school principals were aware of the healthy snack initiative and showed a positive attitude towards it, the majority lacked knowledge about its specific content. Factors underlying compliance with the healthy snacking initiative were related to its characteristics, characteristics of the schools, and external factors such as family habits and advertising. CONCLUSIONS: Results showed that the rationale underlying the selling of products at schools favours the availability of ultra-processed products and constitutes the main barrier for the promotion of healthy dietary habits among children and adolescents. Strategies aimed at facilitating the identification of unhealthy foods and beverages and provision of incentives to canteen managers to modify their offer are recommended.

Metalaxyl Degradation by Mucorales Strains Gongronella sp. and Rhizopus oryzae.

In this study, the degradation of metalaxyl was investigated in the presence of two Mucorales strains, previously isolated from soil subjected to repeated treatments with this fungicide and selected after enrichment technique. Fungal strains were characterised by a polyphasic approach using phylogenetic analysis of the Internal Transcribed Spacer (ITS) gene region, phenotypic characterisation by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) spectral analysis, and growth kinetics experiments. The strains were identified as Gongronella sp. and Rhizopus oryzae. The fungal growth kinetics in liquid cultures containing metalaxyl fits with Haldane model. Under laboratory conditions, the ability of Gongronella sp. and R. oryzae cultures to degrade metalaxyl was evaluated in liquid cultures and soil experiments. Both species were able to: (a) use metalaxyl as the main carbon and energy source; and (b) degrade metalaxyl in polluted soils, with rates around 1.0 mg kg-¹ d-¹. This suggests these strains could degrade metalaxyl in soils contaminated with this fungicide.