Influence of barley inclusion method and protease supplementation on growth performance, nutrient utilisation, and gastrointestinal tract development in broiler starters.

The influence of the method of barley inclusion (fine, coarse and whole barley) in a wheat-based diet and protease supplementation (0 and 0.20 g/kg) on growth performance, nutrient utilisation and gastrointestinal tract development of broilers (d 1 to 21) was evaluated in a 3 × 2 factorial arrangement. Whole barley (WB) grains were ground in a hammer mill to pass through the screen sizes of 2.5 and 8.0 mm to achieve fine (FB) and coarse (CB) barley particle sizes, respectively. A total of 288, one-day-old male broilers were allotted to 36 cages (6 cages/treatment; 8 birds/cage). There was no significant (P > 0.05) interaction between barley inclusion method and protease for any growth performance or nutrient utilisation parameters. Birds fed diets containing CB and WB showed higher (P < 0.05) weight gain, and digestibility of dry matter, nitrogen, calcium, gross energy, and ileal digestible energy compared to those fed FB diets. Compared to the birds fed FB diets, feed per gain was lower (P < 0.05) in birds fed diets made of WB. Fat digestibility of the birds fed CB was higher (P < 0.05) than those fed FB and WB birds. Compared to FB and CB diets, inclusion of WB resulted in heavier (P < 0.05) gizzards but reduced (P < 0.05) gizzard pH. Supplemental protease, however, had no effects (P > 0.05) on growth performance and nutrient utilisation, most likely due to the well balanced digestible amino acids and high inherent digestibility of protein in the basal diet, and/or the presence of exogenous carbohydrase and phytase. In conclusion, the present results showed that the inclusion of coarsely ground and whole barley in a wheat-based diet can enhance nutrient and energy utilisation and is beneficial to the growth performance of young broilers.

High steam-conditioning temperature during the pelleting process impairs growth performance and nutrient utilization in broiler starters fed barley-based diets, regardless of carbohydrase supplementation.

The influence of supplemental carbohydrase (Carb) and conditioning temperature (CT) on growth performance, nutrient utilization and intestinal morphometry of broilers (d 1-21) fed barley-based diets was examined in a 2 × 3 factorial arrangement, evaluating 2 levels of Carb (0 and 150 g/tonne of feed) and three CT (60, 74, and 88°C). A total of 288, 1-day-old male broilers (8 birds/cage; 6 cages/treatment) were used. The activities of endo-1,4-ß- glucanase, endo-1,3 (4)-ß-glucanase and endo-1,4-ß-xylanase in the Carb were 800 BGU/g, 700 BGU/g and 2,700 XU/g, respectively. On d 21, ileal digesta was collected for the determination of nutrient digestibility. There was no significant interaction between Carb and CT for any tested parameter. Supplemental Carb, regardless of CT, increased weight gain (WG; P < 0.05) and reduced feed per gain (F/G; P < 0.001) by 30 g/bird and 6.5 points, respectively. Increasing CT to 88°C reduced (P < 0.05) WG, but increased (P < 0.05) F/G compared to the diets conditioned at 60° and 74°C. Regardless of CT, Carb enhanced (P < 0.05) the digestibility of starch and AMEn by 1.15% and 32 kcal/kg, respectively. Compared to the diets conditioned at 60° and 74°C, CT at 88°C reduced (P < 0.05) digestibility of dry matter, nitrogen, phosphorus, gross energy, and AMEn. Birds fed diets conditioned at 88°C showed lower (P < 0.05) starch digestibility compared to those fed diets conditioned at 60°C. Conditioning at 88°C increased (P < 0.05) jejunal digesta viscosity by 10.2% compared to diets conditioned at 60° and 74°C. Overall, Carb supplementation improved WG, F/G, starch digestibility and AMEn in broilers fed barley-based diets, irrespective of CT applied. Conditioning barley-based diets at 88°C impaired the ability of birds to utilize nitrogen, starch, phosphorus and energy, and consequently deteriorated WG and F/G. The lack of significant interactions between Carb and CT indicated that negative impacts caused by high CT on bird performance and nutrient utilization occurred regardless of Carb enzyme supplementation. Supplemental Carb per se could not remedy the adverse effects of high CT.

The interactive influence of barley particle size and enzyme supplementation on growth performance, nutrient utilization, and intestinal morphometry of broiler starters.

The influence of barley particle size and enzyme supplementation on performance, nutrient and energy utilization, and intestinal morphometry of broiler starters (day 1-21) fed pelleted barley-based diets was evaluated. Two barley particle sizes (fine and coarse) and 4 enzyme treatments (nonsupplemented [control], carbohydrase [0.15 g/kg of feed; Carb], phytase [0.10 g/kg; Phy] and combination of carbohydrase and phytase [0.15 and 0.10 g/kg, respectively; Carb + Phy]) were evaluated in a 2 × 4 factorial arrangement. Fine and coarse barley particles were achieved by grinding whole barley in a hammer mill to pass through 2.0 and 8.0 mm screens, respectively. A total of 384, 1-day-old male broilers (8 birds/cage; 6 cages/treatment) were used. Supplemental enzymes tended (P = 0.056) to increase the weight gain of birds with a synergetic effect from Carb + Phy. The response of feed intake to supplemental enzymes interacted (P < 0.05) with barley particle size, as Phy increased feed intake only in fine barley diets. Both coarse particles and supplemental Carb, either individually or in combination with Phy, reduced feed per gain (P < 0.001). Digestibility of DM, nitrogen, and fat was greater in birds fed coarse barley diets (P < 0.05). Dry matter, starch, fat, and phosphorus digestibility values were improved by supplemental enzymes (P < 0.05). Coarse barley (P < 0.05) and Carb (P < 0.001), either individually or in combination, increased the AMEn. Coarse barley reduced the gizzard pH (P < 0.001). Birds fed diets with supplemental enzymes had shorter jejunum (P < 0.05). Neither the barley particle size nor supplemental enzymes (P > 0.05) affected the jejunal digesta viscosity. In summary, feeding coarse barley particles and supplemental Carb improved the feed efficiency and nutrient and energy utilization. The effects of barley particle size on measured parameters suggest that the particle size effect was preserved even after pelleting. The combination of Carb and Phy tended to improve the weight gain but caused no further improvements in nutrient utilization.

The effect of graded inclusions of waxy starch hull-less barley and a multi-component exogenous carbohydrase on the growth performance, nutrient digestibility and intestinal morphometry of broiler chickens.

1. A 21-d experiment was conducted to investigate the effect of graded inclusions of waxy starch hull-less (WSHL) barley and a multi-component exogenous carbohydrase on the growth performance, nutrient digestibility and intestinal morphometry of broiler chickens. Five levels of WSHL barley inclusion (0, 65, 130, 195 and 260 g/kg) in a wheat-based diet, and two levels of enzyme supplementation (0 and 150 g/tonne of feed) were evaluated in a 5 × 2 factorial arrangement of 10 dietary treatments. All diets were equivalent in metabolisable energy and digestible amino acid content. A total of 400, one-d-old male broilers (five cages/treatment; eight birds/cage) were used in the experiment. 2. Regardless of enzyme supplementation, feed intake declined (P < 0.001) with increasing inclusion of WSHL barley. Increasing levels of WSHL barley (P < 0.001) and supplemental enzyme (P < 0.01) increased gain to feed ratio. 3. Birds fed diets with 0 g/kg WSHL barley showed the lowest (P < 0.001 to 0.01) digestibility for all nutrients except starch. Only starch digestibility was improved (P < 0.05) by enzyme supplementation. The nitrogen-corrected apparent metabolisable energy improved with increasing inclusion of WSHL barley (P < 0.001) and supplemental enzyme (P < 0.001). Increasing inclusion of WSHL barley increased the relative weight of gizzard (P < 0.001) and reduced jejunal digesta viscosity (P < 0.01). Supplemental enzyme (P < 0.001) reduced digesta viscosity. 4. All levels of WSHL barley inclusion improved digestibility of dry matter, nitrogen and fat, whilst energy utilisation improved at inclusions of 130 g/kg WSHL and above, probably due to lowered digesta viscosity and better development of the gizzard. Gain to feed ratio, starch digestibility, energy utilisation and jejunal digesta viscosity can benefit from carbohydrase supplementation in wheat-based diets, regardless of barley inclusion level.

Influence of inclusion level of barley in wheat-based diets and supplementation of carbohydrase on growth performance, nutrient utilisation and gut morphometry in broiler starters.

1. The influence of barley inclusion level and supplementation of a multi-component non-starch polysaccharide degrading enzyme on performance and nutrient utilisation in broilers was investigated. Normal-starch hulled barley was evaluated with five levels of inclusion (0, 141, 283, 424 and 565 g/kg) in a wheat-based diet and two levels of enzyme supplementation (0 and 150 g/tonne of feed; a 5 × 2 factorial arrangement of 10 dietary treatments). All diets were equivalent in metabolisable energy and digestible amino acid contents. A total of 400, one-d old male broilers (five cages/treatment; eight birds/cage) were used in the experiment.2. Regardless of enzyme supplementation, weight gain (WG) increased up to 283 g/kg of barley and was reduced afterwards (P < 0.01). Increasing levels of barley resulted in greater (P < 0.001) gain per feed (G/F). Enzyme addition increased WG (P < 0.05) and G/F (P < 0.001) at each barley inclusion level.3. Birds fed diets with 0 and 565 g/kg barley showed the lowest and highest (P < 0.001to 0.05) digestibility for all nutrients measured, respectively. Digestibility of all nutrients was improved by enzyme supplementation at each barley inclusion level (P < 0.05). The nitrogen-corrected apparent metabolisable energy improved with increasing inclusion of barley (P < 0.001) and supplemental enzyme (P < 0.01). Increasing inclusion of barley increased the relative weight of gizzard (P < 0.001) and reduced jejunal digesta viscosity (P < 0.001). Supplemental enzyme (P < 0.001) reduced digesta viscosity.4. The optimum inclusion level of barley, with respect to growth performance, was 283 g/kg of diet. Increasing barley inclusion improved nutrient and energy utilisation, possibly through lowered digesta viscosity and better function of the gizzard. Feed efficiency and nutrient and energy utilisation can benefit from carbohydrase supplementation in barley-based diets, regardless of barley inclusion level.

Nutritional evaluation of two barley cultivars, without and with carbohydrase supplementation, for broilers: metabolisable energy and standardised amino acid digestibility.

1. Two experiments were conducted to assess the nitrogen-corrected apparent metabolisable energy (AMEn; Exp. 1; 288 Ross 308 male broilers at d 14; 36 cages with eight birds each) and coefficient of standardised ileal digestibility (CSID) of amino acids (AA; Exp. 2; 336 Ross 308 male broilers at d 21; 42 cages with eight birds each) of two barley cultivars for broilers in comparison to wheat, without or with a multi-component non-starch polysaccharide (NSP) degrading enzyme. A 3 × 2 factorial arrangement of treatments was used in both experiments with three types of grains (normal starch hulled barley [NSH], waxy starch hull-less barley [WSHL], and wheat) and two levels of enzyme supplementation (0 and 200 g/tonne of feed). Enzyme supplemented diets contained 406 and 128 of endo-1, 4-ß-xylanase and endo-1, 3 (4)-ß-glucanase units per kg of feed, respectively. 2. Analysis showed that the starch content was higher in NSH (610 g/kg) than in wheat (537 g/kg) and WSHL (554 g/kg), and the composition of starch differed markedly among the grain types. The ß-glucan content was considerably higher in WSHL (68.6 g/kg) compared to NSH (38.5 g/kg) and wheat (7.74 g/kg). The contribution of soluble fraction to the total non-starch polysaccharides was higher in WSHL (38.2%) compared to NSH and wheat (17.1% and 13.3%, respectively). 3. A significant (P < 0.01) interaction was observed between the grain type and enzyme supplementation for AMEn. The WSHL, with the highest content of ß-glucan, showed the greatest response to enzyme supplementation for AMEn. 4. Birds fed wheat- and WSHL-based diets had the highest and lowest CSID of nitrogen and most of AA, respectively, with NSH diets being intermediate. Regardless of grain type, enzyme supplementation increased (P < 0.05) the CSID of nitrogen. 5. These data suggest that ß-glucan content plays an important role in determining the digestibility of nutrients in barley for broilers, resulting in a better feeding value for NSH over WSHL. Supplementation of a multi-component NSP-degrading enzyme can improve the feeding value of barley in broiler diets by increasing the digestibility with the effect being more pronounced in WSHL barley.