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Org Biomol Chem ; 5(14): 2225-33, 2007 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-17609753

RESUMO

De novo design and total chemical synthesis of proteins provides a powerful approach for biological and biophysical studies with the ability to prepare artificial proteins with tailored properties, potentially of importance for biophysical studies, material science, nanobioscience, and as molecular probes. In this paper, the previously developed concept of carbohydrates as templates is employed in the de novo design of model proteins (artificial helix bundles) termed 'carboproteins'. The 4-alpha-helix bundle is a macromolecular structure, where four amphiphilic alpha-helical peptide strands form a hydrophobic core. Here this structure is modified towards achieving metal ion-binding and catalytic activity. We report: (i) test of directional effects from different tetravalent carbohydrate templates, (ii) synthesis and evaluation of carboproteins functionalized with phenol, pyridyl or imidazolyl moieties as potential ligands for metal ion-binding as well as for catalysis. Our results include: (i) support of our previous 'controversial' finding that for some carboproteins the degree of alpha-helicity depends on the template, i.e., that there is, to some extent, a controlling effect from the template, (ii) demonstration of binding of Cu(ii) to tetra-functional carboproteins by electrospray ionization-time of flight-mass spectrometry (ESI-TOF-MS), UV-VIS absorption spectroscopy and size exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS); (iii) a kinetic investigation of the esterase activity.


Assuntos
Esterases/química , Glicoproteínas/síntese química , Metaloproteínas/síntese química , Sequência de Aminoácidos , Carboidratos/química , Quelantes/química , Dicroísmo Circular , Técnicas de Química Combinatória , Cobre/química , Esterases/metabolismo , Glicoproteínas/química , Espectrometria de Massas , Metaloproteínas/química , Dados de Sequência Molecular , Oxirredução , Peptídeos/síntese química , Peptídeos/química
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