RESUMO
The effects of soman, a potent irreversible inhibitor of acetylcholinesterase, on central neuropathology in rats were studied in relation with subsequent spatial memory impairments. In a first step, it was found that, without treatment, neuropathology and learning impairment were observed only in rats which experienced convulsions. Then, treatment consisting of atropine sulfate, and/or TCP and/or NBQX was administered to intoxicated animals at infraanticonvulsant doses to obtain a graded subsequent neuropathology and to appreciate an eventual relation between neuropathology and spatial memory impairment. Thus, a correlation between neuropathology in the hippocampal CA1 region and spatial learning performance was found, the degradation of performance of rat being directly related to the amplitude of their neural damage. A threshold was emphasized : below a certain degree of neural loss, no memory impairment was found. Only treatment with tritherapy (atropine + TCP + NBQX) was able to improve the different parameters of spatial learning, despite no effect on the convulsions of the animals.
Assuntos
Hipocampo/efeitos dos fármacos , Transtornos da Memória/induzido quimicamente , Fenciclidina/análogos & derivados , Quinoxalinas/farmacologia , Soman/toxicidade , Animais , Antídotos/farmacologia , Atropina/farmacologia , Comportamento Animal/efeitos dos fármacos , Antagonistas Colinérgicos/farmacologia , Convulsivantes/toxicidade , Relação Dose-Resposta a Droga , Interações Medicamentosas , Agonistas de Aminoácidos Excitatórios/farmacologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Hipocampo/patologia , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Fenciclidina/farmacologia , Ratos , Ratos Wistar , Comportamento Espacial/efeitos dos fármacos , Taxa de Sobrevida , Fatores de TempoRESUMO
After intracerebral injection, some toxic secreted phospholipases A2 (sPLA2) can induce epileptic seizures which bases are currently ill known. We undertook the detailed study of the central neurotoxicity of paradoxin (PDX), an analog of taipoxin, in rodents. Since literature strongly suggests a high variability in the sPLA2 epileptogenic properties, we compared, in an acute model, PDX with crotoxin (CTX), known to induce seizures and that may bind to similar neuronal receptors. Related toxic enzymes (ammodytoxin A, ATX A, and CTX subunit CB) and the non neurotoxic sPLA2 from pancreas and PLA2 analog ammodytin L (AML) were also tested. Despite being highly neurotoxic, PDX did not induce either convulsions or long-lasting seizure fits. The results obtained with the other enzymes showed that toxic sPLA2s can effectively be differentiated based on two criteria: the presence of cortically recorded epileptic paroxysmal discharges (E) and convulsions (C). We thus propose to classify the toxic sPLA2s into different groups depending on their epileptogenic properties: E-C-(PDX), E+C+ (CTX, CB), and E-C+ (ATX A). The non toxic AML and pancreatic enzyme were E-C-. Moreover, the results obtained with AML, and preliminarily with chemically inhibited CB, suggested that phospholipid hydrolysis is important to trigger seizures and convulsions. However, PDX and CTX that possess highly different epileptogenic properties exerted comparable, although slightly different, catalytic activities. Similarly, histological evaluations of the brain of PDX and CTX-treated rats (H&E staining, GFAP immunodetection, hsp70 and c-fos mRNA detection) did not provide satisfactory clues to explain these large differences. Further studies are strongly required.
Assuntos
Crotoxina/toxicidade , Bloqueadores Neuromusculares/toxicidade , Fosfolipases A/toxicidade , Animais , Venenos Elapídicos , Epilepsia/induzido quimicamente , Masculino , Camundongos , Fosfolipases A/metabolismo , Fosfolipases A2 , Ratos , Ratos WistarRESUMO
NBQX, a specific and potent AMPA receptor antagonist has been found to be neuroprotective in various models of ischemia and to have anticonvulsant properties in different models of epilepsy. In this experiment, the neurobehavioral effects of NBQX were studied. In an open field, an important ataxia was emphasized at a dose of 60 mg/kg. In a swimming task, an increase of the escape latencies was noted on the third day at a dose of 40 mg/kg. In a Morris water maze task, doses devoid of effects on locomotion were used (10, 20, and 30 mg/kg). There was no effect on the acquisition of the task at 10 mg/kg and a slight impairment at 20 mg/kg, but the rats did not learn the task at 30 mg/kg. This impairment was reversible, as shown by the increasing performance of this group without treatment. No impairment was noted in the retention phase of the Morris water maze task. The results are discussed relative to the role of the AMPA receptor in memory processes.
Assuntos
Antagonistas de Aminoácidos Excitatórios/farmacologia , Quinoxalinas/farmacologia , Receptores de AMPA/antagonistas & inibidores , Animais , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Memória/efeitos dos fármacos , Atividade Motora/efeitos dos fármacos , Ratos , Ratos WistarRESUMO
In a nominally calcium-free medium, a toxic phospholipase A2, paradoxin, PDX (1-200nM) was able to significantly decrease glutamate uptake by rat hippocampal mini-slices. Under the same experimental conditions, PDX could also inhibit the reuptake of choline and dopamine, suggesting a nonselective action. Furthermore, we found no evidence of competition between PDX and [3H]L-Aspartate described as a marker of glutamate carrier proteins. A direct blockage of glutamate uptake by binding to the glutamate transporters is thus unlikely to occur. Implication of the free fatty acids (FFAs), or their metabolites, was clearly shown by the total suppression of PDX effect on reuptake in a medium inhibiting its catalytic activity (EGTA/Sr2+ buffer). Moreover, analysis of the FFAs liberated showed a significant increase in polyunsaturated fatty acid (PUFA) levels. Arachidonic acid (AA) concentration reached in the water phase, though in the low micromolar range, may be especially relevant in explaining this effect. Much higher concentrations are found in the membranes and may also participate in the action on reuptake. Evidence for the involvement of FFAs was also provided by the antagonistic, although partial, action of bovine serum albumine (BSA, 1%). Finally, free radicals or eicosanoids did not seem to play a significant role given the persistence of inhibition in the presence of NDGA (1 microM) or indomethacin (10 microM), inhibitors of the two major AA metabolic pathways. Altogether, PDX-induced uptake impairment may thus be related to the direct action of AA and other PUFAs on the glutamate transporter, as well as through less selective actions.
Assuntos
Venenos Elapídicos/enzimologia , Venenos de Peixe/farmacologia , Ácido Glutâmico/metabolismo , Hipocampo/efeitos dos fármacos , Fosfolipases A/metabolismo , Fosfolipídeos/metabolismo , Animais , Ácido Araquidônico/metabolismo , Colina/metabolismo , Corpo Estriado/efeitos dos fármacos , Corpo Estriado/metabolismo , Inibidores de Ciclo-Oxigenase/farmacologia , Dopamina/metabolismo , Ácidos Graxos/metabolismo , Hipocampo/metabolismo , Hidrólise , Inibidores de Lipoxigenase/farmacologia , Masculino , Fosfolipases A2 , Ratos , Ratos WistarRESUMO
Phencyclidine-like drugs are effective against convulsions and brain lesions related to soman intoxication but induce severe side effects. The well tolerated antitussive dextromethorphan (DM) and its metabolite dextrorphan (DX) have antiepileptic and neuroprotective properties that we evaluated in mice against 2 LD50 of soman in a three-drug pretreatment (atropine sulfate and oxime HI-6 plus DM: 20-50 mg/kg or DX: 10-40 mg/kg i.p). Neuroprotection was evaluated by measurement of hippocampal omega 3 binding site density. DM and DX have weak anticonvulsant and neuroprotective activities which are counterbalanced at high doses by an increased mortality due to respiratory distress for DM and by ataxia for DX. Thus DM and DX do not appear to be appropriate for the pretreatment of soman intoxication.
Assuntos
Inibidores da Enzima Conversora de Angiotensina/toxicidade , Anticonvulsivantes/uso terapêutico , Antitussígenos/uso terapêutico , Dextrometorfano/uso terapêutico , Dextrorfano/uso terapêutico , Fármacos Neuroprotetores/uso terapêutico , Animais , Avaliação Pré-Clínica de Medicamentos , Estudos de Avaliação como Assunto , Camundongos , Soman/antagonistas & inibidores , Soman/toxicidadeRESUMO
Alpha-dendrotoxin (alpha-DTx), a snake venom toxin which blocks several types of fast-activating voltage-dependent potassium channels, induces limbic seizures and neuronal damage when injected into the brain. The mechanisms underlying these convulsant and neuropathological actions are not fully understood. We have studied the effects of alpha-DTx on neurotransmitter release and electrical activity in rat hippocampal brain slices and the role of excitatory amino acid receptors in mediating these actions of the toxin. alpha-DTx increased the basal release of acetylcholine, glutamate, aspartate, and GABA in a concentration-dependent manner and induced epileptiform bursting in the CA1 and CA3 regions of the slice. The increase in neurotransmitter release was evident during the first 4 min after toxin addition, whereas the bursting appeared after a concentration-dependent delay (20-40 min with 250 nM toxin). The N-methyl-D-aspartate (NMDA) receptor antagonists AP5 and MK-801 had no effect on the frequency or amplitude of dendrotoxin-induced epileptiform bursts, but the non-NMDA antagonists CNQX and DNQX abolished bursting in both CA1 and CA3 within 4-6 min. In contrast, the toxin-induced increases in neurotransmitter release were not blocked by DNQX. This study has demonstrated that, following exposure to alpha-DTx, there is a rapid increase in the release of neurotransmitters which precedes the onset of epileptiform bursting in the hippocampus. Since DNQX abolished the bursting but had no effect on the increase in neurotransmitter release, these results suggest that DNQX blocks alpha-DTx-induced epileptiform activity by antagonism of postsynaptic non-NMDA receptors.
Assuntos
Venenos Elapídicos/farmacologia , Antagonistas de Aminoácidos Excitatórios/farmacologia , Hipocampo/efeitos dos fármacos , Neurotoxinas/farmacologia , Neurotransmissores/metabolismo , Convulsões/fisiopatologia , 2-Amino-5-fosfonovalerato/farmacologia , 6-Ciano-7-nitroquinoxalina-2,3-diona/farmacologia , Acetilcolina/metabolismo , Potenciais de Ação/efeitos dos fármacos , Animais , Ácido Aspártico/metabolismo , Maleato de Dizocilpina/farmacologia , Venenos Elapídicos/antagonistas & inibidores , Ácido Glutâmico/metabolismo , Hipocampo/metabolismo , Hipocampo/fisiopatologia , Masculino , Neurotoxinas/antagonistas & inibidores , Canais de Potássio/efeitos dos fármacos , Quinoxalinas/farmacologia , Ratos , Ratos Wistar , Receptores de N-Metil-D-Aspartato/efeitos dos fármacos , Receptores de N-Metil-D-Aspartato/fisiologia , Ácido gama-Aminobutírico/metabolismoRESUMO
Previous studies have shown the successive expression of c-fos and hsp70 genes, especially in the hippocampal formation, during soman-induced seizures. In order to detect a possible link between the induction of these two genes, antisense strategies have been used. First, the ability of unilateral intrahippocampal infusion of c-fos antisense oligonucleotides to inhibit ipsilateral, seizure-related, c-FOS-like immunoreactivity, was verified. Second, induction of hsp70 mRNA was investigated using in situ hybridization. Unilateral inhibition of c-fos induction clearly reduced levels of hsp70 mRNA in the c-fos antisense-infused hippocampus relative to the non-infused contralateral side. Infusion of c-fos sense probe or vehicle did not affect bsp70 mRNA induction. This study suggests a role of c-FOS in regulating bsp70 mRNA induction.
Assuntos
Proteínas de Choque Térmico HSP70/biossíntese , Hipocampo/metabolismo , Oligonucleotídeos Antissenso/farmacologia , Proteínas Proto-Oncogênicas c-fos/genética , RNA Mensageiro/biossíntese , Convulsões/metabolismo , Animais , Convulsivantes , Hipocampo/efeitos dos fármacos , Imuno-Histoquímica , Injeções , Masculino , Oligonucleotídeos Antissenso/administração & dosagem , Ratos , Ratos Wistar , Convulsões/induzido quimicamente , SomanRESUMO
The time course of induction of the proto-oncogene c-fos and the inducible heat shock hsp70 gene was studied from 5 minutes to 24 hours at both transcriptional (c-fos and hsp70 mRNA) and translational levels (C-FOS and HSP72 proteins) in the rat hippocampus and piriform cortex (Pir) after soman-induced seizures. Induction of c-fos was noticed as early as 5 minutes after seizures onset in all fields of hippocampal formation (CA1, CA3, CA4, and dentate gyrus) and in piriform cortex. The most intense induction was observed in piriform cortex. A sustained activation of c-fos occurred in Pir and in CA1, CA3, and CA4 areas of hippocampus. Nevertheless, histological analysis showed rare affected neurons in CA4, whereas damage was severe in Pir and in CA1 and CA3 hippocampal subfields. Induction of hsp70 mRNA occurred but was delayed in all areas previously exhibiting c-fos expression. Nevertheless HSP72 protein was never expressed in the structures where injury was high (i.e., CA1 and piriform cortex) and mainly occurred in the less damaged structure (i.e., CA4 area of hippocampus). Regional expression of glial fibrillary acidic protein mRNA was also studied in order to exclude an astroglial origin of the c-fos and hsp70 gene inductions. Our results demonstrated that after soman induced-seizures 1) there was no strict correlation between time course or intensity of neuronal c-fos induction and subsequent neuropathology, and 2) the most lesioned areas did not express HSP72 protein in spite of intense mRNA induction, suggesting that transcriptional and translational events for hsp70 gene might vary according to the severity of seizure insult.
Assuntos
Córtex Cerebral/metabolismo , Proteínas de Choque Térmico HSP70/biossíntese , Hipocampo/metabolismo , Proteínas Proto-Oncogênicas c-fos/biossíntese , Convulsões/metabolismo , Animais , Astrócitos/metabolismo , Córtex Cerebral/citologia , Convulsivantes , Hipocampo/citologia , Processamento de Imagem Assistida por Computador , Imuno-Histoquímica , Hibridização In Situ , Masculino , Neurônios/metabolismo , Ratos , Ratos Wistar , Convulsões/induzido quimicamente , SomanRESUMO
The effects of the inhibitors of endothelial and neuronal nitric oxide (NO) synthases, N-nitro-L-arginine methyl ester (L-NAME) and 7-nitroindazole (7-NI), respectively, and the precursor of NO, glyceryl trinitrate, on soman-induced seizures, lethality, and neuropathology were studied in rats. It was found that pretreatment of rats with L-NAME and 7-NI potentiated the severity of motor convulsions and enhanced lethality produced by soman. On the other hand, glyceryl trinitrate, administered transdermally at doses ranging from 2.5-5 mg/day 1 day before soman, decreased seizure susceptibility and lethality in soman-intoxicated animals. This was accompanied by a subsequent reduction of central neuronal damage 24 h after soman treatment. Pretreatment with glyceryl trinitrate also reversed seizure latency produced by 7-NI treatment during soman intoxication. These results indicate that neuronal NO may play a prominent role in seizures by acting as an anticonvulsant and neuroprotectant in soman intoxication.
Assuntos
Encéfalo/efeitos dos fármacos , NG-Nitroarginina Metil Éster/farmacologia , Óxido Nítrico/farmacologia , Nitroglicerina/farmacologia , Convulsões/fisiopatologia , Soman/farmacologia , Animais , Relação Dose-Resposta a Droga , Masculino , Ratos , Ratos Sprague-DawleyRESUMO
We investigated the time course of GFAP levels in the hippocampal formation during the first 24 h following soman intoxication in rats. GFAP mRNA expression was detected by in situ hybridization. Intense GFAP mRNA expression was present in the molecular layer of the dentate gyrus as early as 6 h after intoxication. This expression was comparatively lower in other dentate gyrus layers and hippocampal CA1, CA3 and CA4 areas and seemed to be related to excessive neuronal activity. Histopathological examination demonstrated that GFAP expression in dentate gyrus is not correlated with lesions. The high astrocytic reactivity in the molecular layer of the dentate gyrus is discussed in relation to the maintenance of the homeostasis of glutamate and of synaptic plasticity in this area during soman intoxication.
Assuntos
Giro Denteado/metabolismo , Proteína Glial Fibrilar Ácida/genética , Hipocampo/metabolismo , Proteínas do Tecido Nervoso/genética , RNA Mensageiro/metabolismo , Convulsões/metabolismo , Animais , Astrócitos/efeitos dos fármacos , Sequência de Bases , Giro Denteado/patologia , Hipocampo/patologia , Masculino , Dados de Sequência Molecular , Ratos , Ratos Wistar , Convulsões/induzido quimicamente , SomanRESUMO
Rat hippocampus and piriform cortex were examined for pathological changes 48 hours after exposure to a convulsant dose of soman. Animals were treated with a low dose of atropine just after soman and were then injected, after 10 or 40 minutes of seizures, with both the anticonvulsant drugs NBQX and TCP. Atropine given alone counteracted the extensive neuronal loss due to soman in both areas without prevention of neuronal suffering. Comparatively, the complete anticonvulsant regimen, given before 40 minutes of seizures, totally prevented hippocampal soman-induced neuropathology. Neurones of piriform cortex were still suffering whatever the time of injection of the drugs. This emphasizes the need for a rapid and definitive anticonvulsant treatment just after soman intoxication to block the subsequent neurotoxic effect of nerve-agent exposure.
Assuntos
Anticonvulsivantes/farmacologia , Convulsões/induzido quimicamente , Convulsões/patologia , Soman , Animais , Atropina/farmacologia , Hipocampo/patologia , Masculino , Condutos Olfatórios/patologia , Fenciclidina/análogos & derivados , Fenciclidina/farmacologia , Quinoxalinas/farmacologia , Ratos , Ratos Wistar , Receptores de AMPA/antagonistas & inibidoresRESUMO
The ability of relatively low doses of atropine, NBQX and TCP administered in combination to prevent or stop seizures induced by soman, was studied in rats. While these drugs injected together early after soman prevented the onset of seizures, their delayed concomitant administration after 5 or 30 min of epileptic activity only mildly attenuated the intensity of seizures. Conversely, a total arrest of epileptic activity was observed in 80 to 100% of animals when NBQX and TCP were given together after 5 to 50 min of seizures to atropine pretreated rats. The large time-window for antiepileptic effectiveness of this 'three drug treatment', provided that atropine is administered early after soman, is discussed in relation to reciprocal potentiations of the antiepileptic effects of atropine, NBQX and TCP in combination.
Assuntos
Atropina/farmacologia , Fenciclidina/análogos & derivados , Quinoxalinas/farmacologia , Receptores de AMPA/antagonistas & inibidores , Convulsões/prevenção & controle , Soman , Animais , Sinergismo Farmacológico , Eletroencefalografia/efeitos dos fármacos , Masculino , Fenciclidina/farmacologia , Ratos , Ratos Wistar , Convulsões/induzido quimicamenteRESUMO
The ability of NBQX, a potent antagonist of AMPA glutamatergic receptors, to prevent or stop seizures induced by the organophosphate soman, an irreversible inhibitor of AChE, was studied in rats. NBQX administered concomitantly with soman prevents the onset of seizures (ED50: 29.2 mg kg-1, i.p.). Administered 5 min after the onset of seizures, NBQX greatly reduces the intensity of the epileptic activity. The same decrease of epileptic activity is observed, in the presence of atropine, when the administration of NBQX is delayed 15 min after the onset of seizures. NBQX thus appears as a promising antiepileptic candidate against soman-induced seizures. The roles of AMPA and muscarinic receptors in the onset and propagation of soman-induced epileptic activity are discussed.
Assuntos
Anticonvulsivantes/uso terapêutico , Quinoxalinas/uso terapêutico , Receptores de AMPA/antagonistas & inibidores , Receptores Muscarínicos/efeitos dos fármacos , Soman/antagonistas & inibidores , Animais , Atropina/uso terapêutico , Quimioterapia Combinada , Masculino , Ratos , Ratos WistarRESUMO
The changes in extracellular gamma-aminobutyric acid (GABA) levels, the modifications in binding capacities of GABA-receptor subtypes A and B and of the Cl- ionophore sites localized in the ionic-channel associated to the GABAA receptors were studied in hippocampus of rats subjected to a convulsive dose of the acetylcholinesterase inhibitor soman. Whereas extracellular GABA levels, just as binding on GABAA and GABAB receptors, were not modified under soman, a significant transient decrease in the binding capacities of the Cl- ionophore site of the GABAA receptor complex occurred within the first 10 min of seizures in CA1, CA3 areas, and in the dentate gyrus with return to basal values after 30 min. Accordingly, a transient decrease of the brain muscimol-gated Cl- influx was observed after 10 min of seizures. An increased ability of diazepam to potentiate the GABAA gated Cl- influx occurred at the same time. Altogether, these data demonstrated that an impairment of the GABAA receptor function occurs at the beginning of seizures. This suggests that a temporary decrease of GABAAergic function may contribute to the onset of seizures.
Assuntos
Compostos Bicíclicos Heterocíclicos com Pontes , Convulsões/induzido quimicamente , Soman/toxicidade , Ácido gama-Aminobutírico/fisiologia , Animais , Autorradiografia , Química Encefálica/efeitos dos fármacos , Compostos Bicíclicos com Pontes/metabolismo , Canais de Cloreto/efeitos dos fármacos , Diazepam/farmacologia , Espaço Extracelular/metabolismo , Hipocampo/metabolismo , Interneurônios/metabolismo , Masculino , Microdiálise , Células Piramidais/metabolismo , Ratos , Ratos Wistar , Receptores de GABA-A/metabolismo , Receptores de GABA-B/metabolismo , Convulsões/metabolismo , Convulsões/fisiopatologia , Ácido gama-Aminobutírico/metabolismoRESUMO
The objective of the present study was to evaluate the anticonvulsant and neuroprotective activities of propentofylline against soman, an irreversible acetylcholinesterase inhibitor. In a first step, the ability of propentofylline to inhibit in vitro the hippocampal evoked release of acetylcholine (ACh) and glutamate (Glu), the two major neurotransmitters involved during soman intoxication, was demonstrated. Propentofylline was then given either at single doses from 0.5 to 25 mg/kg or with repetitive injections at 10 mg/kg to mice subjected to soman. Neither tonic-clonic convulsions induced by soman nor subsequent hippocampal damage were reduced in propentofylline-treated mice. This observation suggested that propentofylline did not inhibit the long-lasting hippocampal release of ACh and Glu under soman.
Assuntos
Anticonvulsivantes/farmacologia , Soman/toxicidade , Xantinas/farmacologia , Acetilcolina/farmacocinética , Animais , Avaliação Pré-Clínica de Medicamentos , Glutamatos/farmacocinética , Masculino , Neurônios/efeitos dos fármacos , Ratos , Ratos WistarRESUMO
Previous investigations have indicated that the measurement of omega 3 (peripheral-type benzodiazepine) binding site densities could be of widespread applicability in the localization and quantification of neural tissue damage in the central nervous system. In the first step of the present study, the suitability of this approach for the assessment of soman-induced brain damage was validated. Autoradiographic study revealed marked increases of omega 3 site densities in several brain areas of convulsing rats 2 days after soman challenge. These increases were well-correlated with the pattern and the amplitude of neuropathological alterations due to soman and closely related to both glial reaction and macrophage invasion of the lesioned tissues. We then used this marker to assess, in mouse hippocampus, the neuroprotective activity against soman-induced brain damage of NBQX and TCP which are respective antagonists of non-NMDA and NMDA glutamatergic receptors. Injection of NBQX at 20 or 40 mg/kg 5 min prior to soman totally prevented the neuronal damage. Comparatively, TCP had neuroprotective efficacy when administered at 1 mg/kg 5 min prior to soman followed by a reinjection 1 h after. These results demonstrate that both NBQX and TCP afford a satisfactory neuroprotection against soman-induced brain damage. Since it is known that the neuropathology due to soman is closely seizure-related, the neuroprotective activities of NBQX and TCP are discussed in relation with the respective roles of non-NMDA and NMDA receptors in the onset and maintenance of soman-induced seizures.
Assuntos
Encefalopatias/induzido quimicamente , Antagonistas de Aminoácidos Excitatórios , Hipocampo , Receptores de GABA-A/metabolismo , Soman/antagonistas & inibidores , Animais , Autorradiografia , Benzoxazinas , Encéfalo/patologia , Encefalopatias/patologia , Hipocampo/patologia , Isoquinolinas , Ligantes , Masculino , Oxazinas , Fenciclidina/análogos & derivados , Fenciclidina/farmacologia , Quinoxalinas/farmacologia , Ratos , Ratos Wistar , Receptores de N-Metil-D-Aspartato/antagonistas & inibidores , Receptores de N-Metil-D-Aspartato/efeitos dos fármacos , Convulsões/patologia , Soman/toxicidadeRESUMO
The effects of intraseptal application of atropine on c-fos proto-oncogene expression related to soman treatment were studied by immunohistochemistry for c-Fos-like proteins. In control rats, 2 h after the onset of convulsion, c-Fos-like immunoreactivity was intense in the piriform and entorhinal cortices, but also in the cingulate, frontoparietal and retrosplenial cortices. In addition, the staining was moderate in the hypothalamus, amygdala and fascia dentata. The intraseptal application of atropine, which prevented soman-induced convulsions, reduced or even blocked c-Fos-like protein production related to soman treatment. This inhibition of Fos induction was significant in most of the limbic structures but also in non-limbic areas. The data in this study strongly suggest that the cholinergic cells of the medial septal area play a key role in soman-induced seizures, and confirm that c-Fos-like protein induction is closely related to neuronal hyperactivity.
Assuntos
Neurônios/fisiologia , Proteínas Proto-Oncogênicas c-fos/metabolismo , Receptores Colinérgicos/fisiologia , Septo Pelúcido/fisiologia , Soman/farmacologia , Animais , Atropina/farmacologia , Comportamento Animal/efeitos dos fármacos , Histocitoquímica , Injeções , Masculino , Ratos , Ratos Wistar , Convulsões/induzido quimicamente , Convulsões/metabolismo , Septo Pelúcido/citologia , Distribuição TecidualRESUMO
The changes in extracellular acetylcholine and glutamate levels were determined, during the course of seizures induced by soman, an irreversible inhibitor of acetylcholinesterase, in the CA1 hippocampal area of rats previously injected with atropine or normal saline into septum. The marked increases observed in soman-treated animals were abolished in rats receiving atropine. These data strongly suggest that, during soman intoxication, septal cholinoceptive cells play a key role in controlling the release of acetylcholine and glutamate in hippocampus. The mechanisms underlying this phenomenon are discussed.
Assuntos
Acetilcolina/metabolismo , Glutamatos/metabolismo , Hipocampo/metabolismo , Receptores Colinérgicos/fisiologia , Convulsões/metabolismo , Soman , Animais , Cromatografia Líquida de Alta Pressão , Eletroquímica , Espaço Extracelular/metabolismo , Ácido Glutâmico , Masculino , Ratos , Ratos Wistar , Receptores Colinérgicos/efeitos dos fármacos , Convulsões/induzido quimicamente , Espectrometria de FluorescênciaRESUMO
Extracellular acetylcholine (ACh) levels were determined, by intracranial microdialysis, in medial septum, amygdala and hippocampus (CA1, CA3, dentate gyrus) of rats during seizures induced by systemic administration of soman (pinacolyl methylphosphonofluoridate), a potent inhibitor of acetylcholinesterase (AChE). In all septo-hippocampal areas a two phase variation was observed: a primary increase in ACh during the pre-seizures period, followed by a decline after 10 to 20 min of seizures and then a second release at 50 min of seizures. In amygdala a progressive increase of the ACh level reached a maximal value at 50 min. ACh levels than returned to basal values in all areas. Hippocampal AChE activity remained totally inhibited throughout the experiment. Possible dynamic phenomena underlying these variations (blood-brain barrier opening, autoregulation of release) are suggested. The present results are compared to previous reports about glutamate changes in the same areas during soman seizures. This comparison gives evidence that in septo-hippocampal areas the glutamatergic system is recruited after an early accumulation of extracellular ACh. The respective roles of ACh and glutamate in triggering and maintenance of soman seizures activity are discussed.
Assuntos
Acetilcolina/metabolismo , Epilepsia Tônico-Clônica/induzido quimicamente , Sistema Límbico/efeitos dos fármacos , Soman/toxicidade , Animais , Diálise , Epilepsia Tônico-Clônica/metabolismo , Sistema Límbico/metabolismo , Masculino , Perfusão , Ratos , Ratos WistarRESUMO
Extracellular amino acid levels in CA3 and CA1 fields of rat hippocampus, an area highly sensitive to seizures, were determined by intracranial microdialysis during seizures induced by systemic administration of soman (o-1,2,2-trimethylpropyl methylphosphonofluoridate), a potent inhibitor of acetylcholinesterase. The glutamate uptake level was determined on another series of animals in hippocampus homogenates. An early and transient increase in the extracellular glutamate level occurred in CA3 within 30 min of seizures, with correlated brief elevations of taurine, glycine and glutamine levels. The glutamate level increased early in CA1, declined and then became more sustained (after 50 min of seizures). Apparent elevations of taurine, glycine and glutamine levels in CA1 accompanied changes in glutamate concentrations. Changes of glutamate level correlated with an increase in the glutamate uptake which rapidly declined after 40 min of seizures. The role of the transient release of glutamate in CA3 and of the sustained release in CA1 in prolonged soman-induced seizures is considered. The correlation between glutamate and other amino acid release is studied.