FT-IR of membranes made with alginate/polylysine complexes. Variations with the mannuronic or guluronic content of the polysaccharides.

FT-IR spectra of polylysine/alginate membranes made with alginate containing various contents of mannuronic or guluronic acid residues have been recorded. The interpretation of the more important absorptions related to functional groups engaged in the complexes have been proposed and discussed using comparisons with spectra of cellulosic films and other published results. Mannuronnic rich alginate seemed to link stronger than guluronic rich alginate to the polylysine molecules which is illustrated by the continuum in absorption between 3000 cm-1 and 2000 cm-1. However, the analysis of the 2000-1000 cm-1 region prompted us to believe that the polymers were engaged in the same basic sort of molecular complexes. Therefore it is necessary that other parameters (either physical, as toughness, porosity, ...) other than variations in molecular structures are studied in order that the biological differences of the membranes may be explained.

Control and isotopic quantification of affinity of antithrombin III for heparin-like surfaces.

Heparin-like materials, characterized by a defined superficial density of functional groups which activate antithrombin III (AT III), when in contact with blood specifically inhibit thrombin as soon as it appears. This paper describes an isotopic method to estimate this density and to visualize the distribution of the affinity sites concerned, both directly with AT III labelled with 125Iodine and indirectly with an anti AT III monoclonal antibody labelled with 111Indium.

Conditions for radioiodination of antithrombin III retaining its biological properties.

In order to obtain a radioiodinated antithrombin III (AT III) with a good labelling yield and optimal biological properties towards heparin, thrombin and its anti-AT III monoclonal antibodies, we compared the classical labelling methods and found them wanting. Thus, we perfected a new labelling procedure which fulfils the above requirements.