A combination of red structural and pigmentary coloration in the eyespot of a copepod.

While the specific mechanisms of colour production in biological systems are diverse, the mechanics of colour production are straightforward and universal. Colour is produced through the selective absorption of light by pigments, the scattering of light by nanostructures or a combination of both. When Tigriopus californicus copepods were fed a carotenoid-limited diet of yeast, their orange-red body coloration became faint, but their eyespots remained unexpectedly bright red. Raman spectroscopy indicated a clear signature of the red carotenoid pigment astaxanthin in eyespots; however, refractive index matching experiments showed that eyespot colour disappeared when placed in ethyl cinnamate, suggesting a structural origin for the red coloration. We used transmission electron microscopy to identify consecutive nanolayers of spherical air pockets that, when modelled as a single thin film layer, possess the correct periodicity to coherently scatter red light. We then performed microspectrophotometry to quantify eyespot coloration and confirmed a distinct colour difference between the eyespot and the body. The observed spectral reflectance from the eyespot matched the reflectance predicted from our models when considering the additional absorption by astaxanthin. Together, this evidence suggests the persistence of red eyespots in copepods is the result of a combination of structural and pigmentary coloration.

Elaborate plumage patterning in a Cretaceous bird.

Integumentary patterns and colors can differentiate species, sexes, and life changes and can inform on habitat and ecology. However, they are rarely preserved in the fossil record. Here, we report on an extremely well-preserved specimen of the Cretaceous bird Confuciusornis with unprecedented complexity, including small spots on the wings, crest, and throat. Morphological and chemical evidence suggest that these patterns are produced by melanin, but unusual preservation prevents assignment of specific colors. Based on comparisons with extant birds, these patterns were likely used for camouflage, although other functions including sexual signaling cannot be ruled out. Our data show that even more elaborate plumage patterns than the spangles in Anchiornis and stripes in Sinosauropteryx were present at a relatively early stage of avian evolution, showing the significance of coloration and patterning to feather evolution.

A new ornithurine from the Early Cretaceous of China sheds light on the evolution of early ecological and cranial diversity in birds.

Despite the increasing number of exceptional feathered fossils discovered in the Late Jurassic and Cretaceous of northeastern China, representatives of Ornithurae, a clade that includes comparatively-close relatives of crown clade Aves (extant birds) and that clade, are still comparatively rare. Here, we report a new ornithurine species Changzuiornis ahgmi from the Early Cretaceous Jiufotang Formation. The new species shows an extremely elongate rostrum so far unknown in basal ornithurines and changes our understanding of the evolution of aspects of extant avian ecology and cranial evolution. Most of this elongate rostrum in Changzuiornis ahgmi is made up of maxilla, a characteristic not present in the avian crown clade in which most of the rostrum and nearly the entire facial margin is made up by premaxilla. The only other avialans known to exhibit an elongate rostrum with the facial margin comprised primarily of maxilla are derived ornithurines previously placed phylogenetically as among the closest outgroups to the avian crown clade as well as one derived enantiornithine clade. We find that, consistent with a proposed developmental shift in cranial ontogeny late in avialan evolution, this elongate rostrum is achieved through elongation of the maxilla while the premaxilla remains only a small part of rostral length. Thus, only in Late Cretaceous ornithurine taxa does the premaxilla begin to play a larger role. The rostral and postcranial proportions of Changzuiornis suggest an ecology not previously reported in Ornithurae; the only other species with an elongate rostrum are two marine Late Cretacous taxa interpreted as showing a derived picivorous diet.