RESUMO
The abdominal cavity of male albino rats, average weight 250 g, was opened and the duodenum approached where the ductus choledochus joins. Pancreatic ducts empty into the lower third of the choledochus. The ductus choledochus was cannulated with a Teflon catheter beneath the liver and its other end was brought out at the neck of the rat. Tissue glue was introduced into the lower part of the choledochus towards the liver. Another Teflon catheter was introduced into the duodenum, its second end brought out at the neck and connected with the first catheter by means of a metal tube, thus preserving bile flow from the liver to the duodenum. Tissue glue injection results in complete atrophy of the exocrine pancreas within 2-15 days. The islets of Langerhans remained intact.
Assuntos
Pancreatectomia/métodos , Adesivos Teciduais/administração & dosagem , Animais , Cateterismo/métodos , Ducto Colédoco/cirurgia , Masculino , Pâncreas/anatomia & histologia , Ductos Pancreáticos/cirurgia , Ratos , Ratos EndogâmicosRESUMO
The Langerhans' islets of the frog Rana ridibunda are built up of A, B, and D cells like other vertebrates. The phospholipids (Baker's test) are located in the capillary pole of the B-Cells, where also beta-granules are present. In the cells of the endocrine pancreas there is found also some quantity of glycogen, but the reaction for zink is negative. The quantity of RNA in the cytoplasme of the islet's cells is minimum.
Assuntos
Ilhotas Pancreáticas/anatomia & histologia , Rana ridibunda/anatomia & histologia , Ranidae/anatomia & histologia , Animais , Feminino , Glicogênio/análise , Histocitoquímica , Ilhotas Pancreáticas/análise , Masculino , Ácidos Nucleicos/análise , Fosfolipídeos/análise , Zinco/análiseRESUMO
The ultrastructural localization of AcPase, AlkPase, ATPase, TPPase, NDPase and G-6-Pase in rat pancreatic B-cells is studied. The plasmolemma is reactive to AlkPase, ATPase, and NDPase. The concave part of the Golgi complex and the lysosomes show AcPase and TPPase activity. In the halo of some beta-granules located near to the Golgi area the AcPase activity is also present. The cisterns of the endoplasmic reticulum are positive for G-6-Pase. So each of the phosphatases has a well expressed ultrastructural localization. It is assumed that this enzyme specificity is connected with the specific organelle's metabolism. Some considerations are made for the possible role of each enzyme in the B-cell physiology.