RESUMO
A new alkaloid of Aconitum karacolicum Rapcs, from the Ranunculaceae family, collected in Kirghizstan, was isolated from the roots of this plant, using a purification scheme based upon its in vitro antiproliferative properties against three human tumour cell lines in culture. Structural identification was performed using high resolution MS-MS mass spectrometry and (1)H, (13)C, 2D NOESY NMR spectroscopy analysis. This compound consists of a 14-benzoylaconine moiety substituted on C-8 by an azeloyl chain. It presents in vitro cytotoxicity with an IC(50) of about 10-20 microM, which warrants further investigation on its possible interest in cancer chemotherapy.
Assuntos
Aconitina/análogos & derivados , Aconitum/química , Alcaloides/química , Alcaloides/farmacologia , Raízes de Plantas/química , Aconitina/química , Aconitina/isolamento & purificação , Aconitina/toxicidade , Alcaloides/isolamento & purificação , Alcaloides/toxicidade , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Humanos , Concentração Inibidora 50 , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Estrutura MolecularRESUMO
The application of the refocused-decoupled INEPT pulse sequence in 13C-NMR spectroscopy allows, in conjonction with computer simulations, a straightforward editing of the spectra of a raw mixture of glycosidic compounds.
