The embryonated egg: a practical target for genetic based advances to improve poultry production.

The embryonated avian egg is an attractive target for applying technology-based advances to improve poultry production. There are a number of reasons for this. First, the egg is immobile and can be easily accessed by high-speed automated equipment such as the commercial egg injection system used for vaccination of broilers worldwide. Second, due to successful breeding techniques, the embryonic period now composes 30 to 40% of a broiler's total lifespan, underscoring the importance of this window in the bird production life cycle. Third, the period of incubation involves rapid development from a ball of 40,000 to 60,000 undifferentiated blastodermal cells to a fully formed chick and associated extra-embryonic compartments in 21 d, allowing development of novel approaches for improving poultry production. Some of these novel approaches will be discussed in this paper and include gender discrimination of embryos and the possibility of changing the breeding paradigm through introduction of undifferentiated cells such as avian blastodermal or embryonic stem cells.

The effect of in ovo oocyst or sporocyst inoculation on response to subsequent coccidial challenge.

A trial was conducted to investigate the effects of in ovo Eimeria maxima inoculation on response to subsequent posthatch challenge with E. maxima. The in ovo treatments were arranged in a 4 x 2 factorial with four in ovo inoculation sites (air cell, amnion, yolk sac, and allantois) and two parasite forms (oocyst and sporocyst). Four control treatments included an uninoculated (naive) unchallenged group, a naive challenged group, and two posthatch inoculated challenged groups. Chicks were challenged by crop intubation with 50,000 sporulated E. maxima oocysts 10 d posthatch. On Day 8 postchallenge, feed intake was determined and birds were weighed and lesions scored. During the brooding period, oocysts were isolated from the fecal material of chicks receiving in ovo administration of sporocysts in the amnion and sporocysts or oocysts in the yolk sac. Posthatch inoculated chicks had gain and feed:gain ratios similar to those of naive unchallenged chicks. Gain, feed:gain ratio, lesion scores, and oocyst shedding of chicks inoculated in ovo were similar to those of naive, challenged chicks. Although there was some indication that parasites introduced in ovo may complete their life-cycle within the developing chick, this experiment provided no evidence that in ovo administration of either E. maxima oocysts or sporocysts will protect birds from subsequent coccidial challenge. Contrarily, inoculating chicks on day of hatch with a single oral dose of E. maxima oocysts provided significant protection against subsequent coccidial challenge.

Effect of cadmium and other metal cations on in vitro Leydig cell testosterone production.

In vivo assessment of toxicant action on Leydig cell function is subject to homeostatic mechanisms which make it difficult to determine whether any changes seen in serum testosterone (T) concentration are due to extragonadal endocrine alterations or to a direct effect on the Leydig cell. For example, metal cations administered in vivo have been shown to depress serum T concentration and alter serum concentrations of pituitary hormones in laboratory animals. The studies reported here use a testicular cell culture technique to evaluate Leydig cell testosterone biosynthesis in the presence of several metal cations. To determine the site of toxic action, the Leydig cells were stimulated to produce testosterone by using human chorionic gonadotrophin (hCG), dibutyl cyclic adenosine monophosphate (db-cAMP), or several substrates required for the biosynthesis of testosterone. hCG was chosen because resultant T production requires an intact membrane receptor and db-cAMP was used to test for post LH receptor defects caused by the metals. The other substrates were chosen to isolate the effect of metals on enzymatic pathways. Collagenase dispersed testicular cells (15% Leydig cells) were incubated with metal cations (1 to 5000 microM) for 3 hr in the absence and presence of maximally stimulating concentrations of hCG, db-cAMP, 20 alpha-hydroxycholesterol (HCHOL), or pregnenolone (PREG), and T concentration was determined by radioimmunoassay. In one separate experiment we also tested the effect of the substrates progesterone, 17 alpha-hydroxy-progesterone, and androstenedione on Cd2(+)-treated Leydig cells. The results show no change in Leydig cell viability with any metal cation treatment during the 3-hr incubation. Ca2+, Cr3+, Fe3+, Mg2+, Na+, or Pb2+ had no effect on stimulated testosterone. Dose-response depression in both hCG- and db-cAMP-stimulated T production were seen with Cd2+, Co2+, Cu2+, Hg2+, Ni2+, and Zn2+ treatment. Surprisingly, Cd2+, Co2+, Ni2+, and Zn2+, which caused a depression in hCG- and db-cAMP-stimulated T production, caused significant increases in HCHOL- and PREG-stimulated T production over untreated and similarly stimulated cultures. This indicates that these cations may act at multiple sites within the Leydig cell.

Comparison of age-related changes in in vivo and in vitro measures of testicular steroidogenesis after acute cadmium exposure in the Sprague-Dawley rat.

Previous reports have demonstrated that cadmium-(Cd-) induced testicular necrosis is an age-dependent process. However, little information exists on age-related intestitial cell (IC) damage in the rat after acute exposure to Cd. In this study in vitro and in vivo measures of testicular damage were utilized to compare the sensitivity of these measures and to further investigate age-related Cd-induced testicular damage. Testes, epididymides, and seminal vesicle weights, serum testosterone (sT), hCG-stimulated sT, and basal and stimulated IC testosterone (T) production were compared in rats 21 d following an injection of 2 mg Cd/kg at 9, 37, 67, and 97 d of age. The only Cd-related change noted for immature rats was an 84% reduction in sT. In rats injected when 37 d old, hCG-stimulated sT and epididymides and seminal vesicle weights, although depressed, were not significantly altered. However, all other measurements were significantly depressed. All measures of testicular damage were significantly depressed in rats injected at 67 and 97 d of age. Overall, in vitro measures were more sensitive indicators of Cd-induced testicular damage than in vivo measures. However, sT and hCG-stimulated sT appeared to be useful indicators of Cd effects on the pituitary-gonadal axis. ICs from immature rats (9 d old) were unaffected by Cd exposure, while stimulated T reproduction in ICs from 37-, 67-, and 97-d-old animals was reduced at least 50%. The severity of Cd-induced testicular damage increased with age for all variables measured.

Effect of prefeeding on physiological parameters associated with turkey poult mortality.

A commercially available antibiotic and nutrient solution was prefed by gavage into the crops of newly hatched poults, which were then brooded under standard conditions of continuous lighting, 35-C temperatures, and ad libitum feed. Treated poults exhibited higher feed consumption, hematocrits, and total red blood cells during the week following the prefeeding regime. Prefeeding partially ameliorated the typically low hematological parameters that have been associated previously with early poult mortality. The results of this study suggest that prefeeding an antibiotic and nutrient solution significantly improves the welfare of poults during the 1st week after hatching.

The posthatch physiology of the turkey poult--I. Growth and development.

Daily body, heart, liver, spleen, pancreas and bursa of Fabricius weights of developing turkey poults were measured. Neither organ nor body weight was affected by sex of the poults during the first 10 days posthatch. Decreases in growth were apparent between days 3 and 6 or 7 and 9 posthatch and coincided with peaks in early mortality.

The posthatch physiology of the turkey poult--II. Hematology.

Packed cell volume, total leukocytes, total erythrocytes, hemoglobin, mean cell volume and mean corpuscular hemoglobin concentration of the poult were analyzed daily for the first 10 days posthatch. Sexually dimorphic effects on hematological parameters were not apparent, but there was a sex X day interaction for all parameters except hemoglobin indicating that males were slower to develop/respond to critical stimuli. The study revealed a latency in production of erythrocytes and leukocytes in posthatch males and females which coincided temporally with early poult mortality.

Effect of phlebotomy on reticulocyte numbers in Japanese Quail.

Hematological changes were studied in response to a phlebotomy of 30% of the estimated total blood volume in 5-week-old Coturnix coturnix japonica. Blood samples, femoral bone marrow samples, and blood and marrow erythroid cell differential counts were collected at 0 (nonphlebotomized), 1, 3, 6, 24, and 72 hr postphlebotomy. The samples were collected only once from each bird. Total peripheral erythrocyte numbers, percent hematocrit, and hemoglobin levels were decreased at 1, 3, 6, and 24 hr. The relative percentage of peripheral erythrocytes with little or no reticulum was not affected by phlebotomy. With the exception of mean cellular hemoglobin concentration, all of the hematological values measured in the peripheral blood had returned to nonphlebotomized, 0 hr levels at 72 hr postphlebotomy. However, in the bone marrow, a marked reticulocytosis was found at 72 hr postphlebotomy.