El grosor del músculo liso de la pelvis renal indica el curso postoperatorio del tratamiento de la obstrucción de la unión ureteropélvica / Thickness of the Renal Pelvis Smooth Muscle Indicates the Postoperative Course of Ureteropelvic Junction Obstruction Treatment

Objetivo: Investigar la relación entre los resultados histopatológicos y el curso postoperatorio de niños tratados quirúrgicamente por obstrucción de la unión ureteropélvica (UUP). Material y métodos: Se examinó retrospectivamente a 28 pacientes operados por obstrucción unilateral de la UUP desde 1998 hasta 2005 con muestras histopatológicas suficientes, así como su seguimiento postoperatorio. Se tintaron las muestras utilizando la elástica van Gieson para diferenciar el músculo liso del colágeno y la elastina. El seguimiento postoperatorio incluyó la ecografía renal (EcoR) y estudios de renograma diurético. Resultados: 12 pacientes con un grosor medio del músculo liso de la pelvis renal (GmMLPR) de 136,97 ± 34,17 mejoraron en 6 meses después de la operación. Nueve pacientes que mejoraron 9 meses después tenían un GmMLPR de 173,61 ± 33,91. Los 7 pacientes restantes, al duodécimo mes, tenían un GmMLPR de 258,78 ± 96,09. La correlación entre el músculo liso de la pelvis renal y el tiempo de mejoría postoperatoria fue extremadamente significativo (p = 0,7928; m < 0,0001). Conclusión: El grosor del músculo liso de la pelvis renal está considerablemente correlacionado con el curso postoperatorio de los pacientes con obstrucción de la UUP, y se puede utilizar como herramienta de pronóstico del comienzo de su mejoría (AU)

Objetive: To investigate the relationship between the histopathologic findings and the postoperative course of children surgically treated for ureteropelvic junction (UPJ) obstruction. Material and methods: Twenty-eight patients operated for unilateral UPJ obstruction from 1998 to 2005 with adequate histopathologic specimens and postoperative follow up were retrospectively reviewed. Specimens were stained using elastic van Geisson to differentiate smooth muscle from collagen and elastin. Postoperative follow up included renal ultrasound (U/S) and diuretic renogram studies. Results: Twelve patients with mean renal pelvis smooth muscle thickness (mRPSMT) of 136.97 ± 34.17 improved on the 6th postoperative month. Nine patients that improved after 9 months postoperatively had mRPSMT=173.61 ± 33.91. The rest 7 patients that improved on the 12th postoperative month had mRPSMT=258.78 ± 96.09. Correlation between renal pelvis smooth muscle and time of postoperative improvement was extremely significant (r = 0.7928, p < 0.0001). Conclusion: The thickness of the renal pelvis smooth muscle is significantly correlated to the postoperative course of patients with UPJ obstruction and can be used as a prognostic tool for the onset of their improvement (AU)

[Thickness of the renal pelvis smooth muscle indicates the postoperative course of ureteropelvic junction obstruction treatment]. / El grosor del músculo liso de la pelvis renal indica el curso postoperatorio del tratamiento de la obstrucción de la unión ureteropélvica.

OBJECTIVE: To investigate the relationship between the histopathologic findings and the postoperative course of children surgically treated for ureteropelvic junction (UPJ) obstruction. MATERIAL AND METHODS: Twenty-eight patients operated for unilateral UPJ obstruction from 1998 to 2005 with adequate histopathologic specimens and postoperative follow up were retrospectively reviewed. Specimens were stained using elastic van Geisson to differentiate smooth muscle from collagen and elastin. Postoperative follow up included renal ultrasound (U/S) and diuretic renogram studies. RESULTS: Twelve patients with mean renal pelvis smooth muscle thickness (mRPSMT) of 136.97 ± 34.17 improved on the 6(th) postoperative month. Nine patients that improved after 9 months postoperatively had mRPSMT=173.61 ± 33.91. The rest 7 patients that improved on the 12(th) postoperative month had mRPSMT=258.78 ± 96.09. Correlation between renal pelvis smooth muscle and time of postoperative improvement was extremely significant (r = 0.7928, p < 0.0001). CONCLUSION: The thickness of the renal pelvis smooth muscle is significantly correlated to the postoperative course of patients with UPJ obstruction and can be used as a prognostic tool for the onset of their improvement.

Smooth muscle cell differentiation in the processus vaginalis of children with hernia or hydrocele.

PURPOSE: Incomplete obliteration of the processus vaginalis (PV) in children with inguinal hernia or hydrocele has recently been proposed to relate to smooth muscle cell (SMC) persistence. The aim of this study was to evaluate the diversity and differentiation of smooth muscle phenotypes in sacs associated with inguinal hernia and hydrocele through the expression of alpha-smooth muscle actin (SMA), h-caldesmon, desmin, and vimentin. METHODS: Sacs associated with male hernia (n = 22), female hernia (n = 8), and hydrocele (n = 10) were immunohistochemically evaluated using monoclonal antibodies against SMA, h-caldesmon, desmin, and vimentin. Peritoneal samples (male, 4; female, 3) and obliterated PV (male, 3) obtained from age-matched patients served as controls. Expressions according to the groups were compared through chi-squared test, and P values less than 0.05 were considered to be statistically significant. RESULTS: Immunohistochemistry did not shown the presence of SMCs in control samples. The expression of SMA, desmin, and h-caldesmon did not differ among sacs obtained from patients with inguinal hernia and hydrocele. However, strong expression of vimentin in SMCs within sacs obtained from patients with hydrocele in comparison with sacs from male patients with inguinal hernia were observed. CONCLUSIONS: Our results indicate that sacs from patients with inguinal hernias and especially from male inguinal hernias have fully differentiated SMCs. On the other hand SMCs in sacs obtained from boys with hydrocele are in an intermediate state of differentiation-dedifferentiation. This phenotypic modulation may represent attempted apoptosis of SMCs, since sacs more sensitive to apoptosis appeared to have more dedifferentiated SMCs. It also probably depicts the differing influence of sympathetic and parasympathetic tonuses during the descent of the testis and the obliteration of PV.

Transition Metal Derivatives of a ((Dimethylamino)ethyl)cyclopentadienyl Ligand. Synthesis and Structures of Amino-Containing Cyclopentadienyl Derivatives of Cobalt(I) and -(III) Including Water-Soluble Compounds.

The synthesis of monometallic cobalt(III) and -(I) complexes of ((dimethylamino)ethyl)cyclopentadienyl are reported. The presence of the basic amino group facilitates these synthesis using the corresponding cyclopentadiene complexes as starting material. A cobaltocenium green complex [{eta(5)-C(5)H(4)(CH(2))(2)N(H)Me(2)}(2)Co(III)](3+)(Cl(-))(3) (3) was obtained from C(5)H(5)(CH(2))(2)NMe(2) (1) or from its salt M[C(5)H(4)(CH(2))(2)NMe(2)] (M = Na, Li) (2) upon reaction with Co(II)Cl(2) in THF. The structure of the complex [{(eta(5)-C(5)H(4)(CH(2))(2)NMe(2))(eta(5)-C(5)H(4)(CH(2))(2)N(H)Me(2))}Co(I)(II)](PF(6))(2) (4), prepared from 3 by treatment with NH(4)PF(6) in aqueous solutions, was solved in the triclinic space group P&onemacr; with one molecule in the unit cell, the dimensions of which were a = 6.314(2) Å, b = 7.137(2) Å, c = 13.452(2) Å, alpha = 103.66(2) degrees, beta = 90.25(2) degrees, gamma = 92.89(2) degrees, and V = 588.2(3) Å(3). Adjacent molecules in the unit cell of 4 are hydrogen bonded via a H(+) through their -NMe(2) side chains. The reaction of Co(2)(CO)(8) with C(5)H(5)(CH(2))(2)NMe(2) (1) leads to the formation of [{eta(5)-C(5)H(4)(CH(2))(2)NMe(2)}Co(I)(CO)(2)] (5). Treatment of 5 with HBF(4) in ether solutions yielded [{eta(5)-C(5)H(4)(CH(2))(2)N(H)Me(2)}Co(I)(CO)(2)](+)BF(4)(-) (6). Oxidation of 5 with I(2) or Cl(2) gas yielded [{eta(5)-C(5)H(4)(CH(2))(2)NMe(2)}Co(III)I(2)] (7a) and [{eta(5)-C(5)H(4)(CH(2))(2)NMe(2)}Co(III)Cl(2)] (7b). Addition of HBF(4) to complex 7a resulted in the breaking of the Co(III)-NMe(2) bond, producing the dimeric complex [{(eta(5)-C(5)H(4)(CH(2))(2)N(H)Me(2))Co(III)I(2)}(2)](2+)(BF(4)(-))(2) (9). The bridged diiodo dimer 10, [{(eta(5)-C(5)H(4)(CH(2))(2)NMe(2))CoI}(2)](2+)(BF(4)(-))(2), on the other hand, could be obtained from complex 7a upon addition of AgBF(4) in CH(2)Cl(2).