RESUMO
Human chorionic gonadotrophin (HCG) stimulates the rate of vitellogenin synthesis and release by fat bodies in puberal females of the marine Crustacean isopods Idotea balthica basteri. Moreover this effect is specific for vitellogenin: since 48 hr after the treatment, the rate of total protein synthesis, in treated females, is not significantly different from that of females injected with physiological medium, or with denaturated gonadotrophin.
Assuntos
Tecido Adiposo/efeitos dos fármacos , Gonadotropina Coriônica/farmacologia , Crustáceos/efeitos dos fármacos , Lipoproteínas/biossíntese , Vitelogeninas/biossíntese , Tecido Adiposo/metabolismo , Animais , Proteínas/metabolismoRESUMO
Synthesis and release of vitellogenin in the crustacean Isopoda, Porcellio dilatatus, were maximal during premolt. At that time, diurnal variations occurred in the haemolymph protein level and in the synthesis and release of vitellogenin and other proteins in the haemolymph. The pattern of variations was always identical and bimodal: the minima were at dusk and at dawn when the maxima occurred in the middle of the day and of the night. These variations were highly significant, and the diurnal means were significantly higher than the nocturnal means.
Assuntos
Proteínas Sanguíneas/biossíntese , Ritmo Circadiano , Crustáceos/metabolismo , Hemolinfa/metabolismo , Lipoproteínas/biossíntese , Vitelogeninas/biossíntese , Animais , Proteínas Sanguíneas/análise , Feminino , Vitelogeninas/sangueRESUMO
This paper investigates vitellogenin synthesis in the fat body of the female marine crustacean Isopoda, Idotea balthica basteri, during vitellogenesis. The fat bodies were incubated in a labelled medium; one of the samples was treated with an antiserum against vitellogenin and the antigen-antibody complex counted for radioactivity. We assumed this radioactivity to be due to vitellogenin synthesis. It is indicated that vitellogenin may account for most of the protein synthesized by the fat body during vitellogenesis. The first peak of vitellogenin synthesis was observed in the early stages of vitellogenesis (molting cycle period C), but the major increase in that level appeared during stage 2 of vitellogenesis (molting cycle period D, stage D1). Before laying (and molt), the fat body incorporated less.