Beneficial Interactive Effects Provided by an Arbuscular Mycorrhizal Fungi and Yeast on the Growth of Oenothera picensis Established on Cu Mine Tailings.

Phytoremediation, an environmentally friendly and sustainable approach for addressing Cu-contaminated environments, remains underutilized in mine tailings. Arbuscular mycorrhizal fungi (AMF) play a vital role in reducing Cu levels in plants through various mechanisms, including glomalin stabilization, immobilization within fungal structures, and enhancing plant tolerance to oxidative stress. Yeasts also contribute to plant growth and metal tolerance by producing phytohormones, solubilizing phosphates, generating exopolysaccharides, and facilitating AMF colonization. This study aimed to assess the impact of AMF and yeast inoculation on the growth and antioxidant response of Oenothera picensis plants growing in Cu mine tailings amended with compost. Plants were either non-inoculated (NY) or inoculated with Meyerozyma guilliermondii (MG), Rhodotorula mucilaginosa (RM), or a combination of both (MIX). Plants were also inoculated with Claroideoglomus claroideum (CC), while others remained non-AMF inoculated (NM). The results indicated significantly higher shoot biomass in the MG-NM treatment, showing a 3.4-fold increase compared to the NY-NM treatment. The MG-CC treatment exhibited the most substantial increase in root biomass, reaching 5-fold that in the NY-NM treatment. Co-inoculation of AMF and yeast influenced antioxidant activity, particularly catalase and ascorbate peroxidase. Furthermore, AMF and yeast inoculation individually led to a 2-fold decrease in total phenols in the roots. Yeast inoculation notably reduced non-enzymatic antioxidant activity in the ABTS and CUPRAC assays. Both AMF and yeast inoculation promoted the production of photosynthetic pigments, further emphasizing their importance in phytoremediation programs for mine tailings.

Transcriptome Analysis of Sweet Cherry (Prunus avium L.) Cultivar 'Lapins' upon Infection of Pseudomonas syringae pv. syringae.

Bacterial canker caused by Pseudomonas syringae pv. syringae (Pss) is responsible for substantial loss to the production of sweet cherry in Chile. To date, the molecular mechanisms of the Pss-sweet cherry interaction and the disease-related genes in the plant are poorly understood. In order to gain insight into these aspects, a transcriptomic analysis of the sweet cherry cultivar 'Lapins' for differentially expressed genes (DEGs) in response to Pss inoculation was conducted. Three Pss strains, A1M3, A1M197, and 11116_b1, were inoculated in young twigs, and RNA was extracted from tissue samples at the inoculation site and distal sections. RNA sequencing and transcriptomic expression analysis revealed that the three strains induced different patterns of responses in local and distal tissues. In the local tissues, A1M3 triggered a much more extensive response than the other two strains, enriching DEGs especially involved in photosynthesis. In the distal tissues, the three strains triggered a comparable extent of responses, among which 11116_b1 induced a group of DEGs involved in defense responses. Furthermore, tissues from various inoculations exhibited an enrichment of DEGs related to carbohydrate metabolism, terpene metabolism, and cell wall biogenesis. This study opened doors to future research on the Pss-sweet cherry interaction, immunity responses, and disease control.

Genome-Wide Identification and Gene Expression Analysis of Sweet Cherry Aquaporins (Prunus avium L.) under Abiotic Stresses.

Aquaporins (AQPs) are integral transmembrane proteins well known as channels involved in the mobilization of water, small uncharged molecules and gases. In this work, the main objective was to carry out a comprehensive study of AQP encoding genes in Prunus avium (cv. Mazzard F12/1) on a genome-wide scale and describe their transcriptional behaviors in organs and in response to different abiotic stresses. A total of 28 non-redundant AQP genes were identified in Prunus spp. Genomes, which were phylogenetically grouped into five subfamilies (seven PIPs, eight NIPs, eight TIPs, three SIPs and two XIPs). Bioinformatic analyses revealed a high synteny and remarkable conservation of structural features among orthologs of different Prunus genomes. Several cis-acting regulatory elements (CREs) related to stress regulation were detected (ARE, WRE3, WUN, STRE, LTR, MBS, DRE, AT-rich and TC-rich). The above could be accounting for the expression variations associated with plant organs and, especially, each abiotic stress analyzed. Gene expressions of different PruavAQPs were shown to be preferentially associated with different stresses. PruavXIP2;1 and PruavXIP1;1 were up-regulated in roots at 6 h and 72 h of hypoxia, and in PruavXIP2;1 a slight induction of expression was also detected in leaves. Drought treatment strongly down-regulated PruavTIP4;1 but only in roots. Salt stress exhibited little or no variation in roots, except for PruavNIP4;1 and PruavNIP7;1, which showed remarkable gene repression and induction, respectively. Interestingly, PruavNIP4;1, the AQP most expressed in cherry roots subjected to cold temperatures, also showed this pattern in roots under high salinity. Similarly, PruavNIP4;2 consistently was up-regulated at 72 h of heat and drought treatments. From our evidence is possible to propose candidate genes for the development of molecular markers for selection processes in breeding programs for rootstocks and/or varieties of cherry.

Keep Calm and Survive: Adaptation Strategies to Energy Crisis in Fruit Trees under Root Hypoxia.

Plants are permanently facing challenges imposed by the environment which, in the context of the current scenario of global climate change, implies a constant process of adaptation to survive and even, in the case of crops, at least maintain yield. O2 deficiency at the rhizosphere level, i.e., root hypoxia, is one of the factors with the greatest impact at whole-plant level. At cellular level, this O2 deficiency provokes a disturbance in the energy metabolism which has notable consequences on the yield of plant crops. In this sense, although several physiological studies describe processes involved in plant adaptation to root hypoxia in woody fruit trees, with emphasis on the negative impacts on photosynthetic rate, there are very few studies that include -omics strategies for specifically understanding these processes in the roots of such species. Through a de novo assembly approach, a comparative transcriptome study of waterlogged Prunus spp. genotypes contrasting in their tolerance to root hypoxia was revisited in order to gain a deeper insight into the reconfiguration of pivotal pathways involved in energy metabolism. This re-analysis describes the classically altered pathways seen in the roots of woody fruit trees under hypoxia, but also routes that link them to pathways involved with nitrogen assimilation and the maintenance of cytoplasmic pH and glycolytic flow. In addition, the effects of root hypoxia on the transcription of genes related to the mitochondrial oxidative phosphorylation system, responsible for providing adenosine triphosphate (ATP) to the cell, are discussed in terms of their roles in the energy balance, reactive oxygen species (ROS) metabolism and aerenchyma formation. This review compiles key findings that help to explain the trait of tolerance to root hypoxia in woody fruit species, giving special attention to their strategies for managing the energy crisis. Finally, research challenges addressing less-explored topics in recovery and stress memory in woody fruit trees are pointed out.

Involvement of aquaporin NIP1;1 in the contrasting tolerance response to root hypoxia in Prunus rootstocks.

Prunus species have been classified as moderately sensitive to root hypoxia, but with a certain intrageneric tolerance degree to oxygen deficiency. Previously, RNA-seq analysis described the transcriptomic reconfiguration of two Prunus rootstocks contrasting to root hypoxia, which included the shift from aerobic to anaerobic metabolism. Here, we studied the relationship between lactate accumulation and the functionality of an aquaporin differentially expressed in 'Mariana 2624', a plum-based (Prunus cerasifera x Prunus munsoniana) rootstock tolerant to root hypoxia stress, and 'Mazzard F12/1', a cherry-based (Prunus avium) rootstock sensitive to root hypoxia stress. In the root hypoxia-sensitive rootstock, higher levels of lactate and LDH1 gene expression were found in roots exposed to oxygen deprivation. Concomitantly, we detected an increase in the mRNA abundance of Prunus spp. NIP1;1, a putative lactic acid transporter. Intriguingly, the high expression of PruavNIP1;1 is not linked to a lower lactic acid content in the roots of 'Mazzard F12/1'. To study this phenomenon, we calculated the force required for the transit of a lactic acid molecule through Prunus spp. NIP1;1 channels. Comparing the calculated forces, we identified steric hindrances in PruavNIP1;1 given by the residues Phe107 and Trp88 in the NPA region and ar/R filter, respectively. The functionality of both channels was corroborated by the restoration of the lactic acid transport and the differential lactic acid sensitive-phenotypes of the yeast strain Δjen1 complemented with PruavNIP1;1 and PrucxmNIP1;1. Our findings provide new insights into the mechanisms involved in determining hypoxia tolerance between closely related species, such as plum and cherry.

Método self-healing como estratégia de promoção à saúde e reabilitação de pessoas com esclerose múltipla no contexto da terapia ocupacional / Self-healing method as strategy to promote health and rehabilitation of people with multiple sclerosis in the context of occupational therapy

Introdução: A esclerose múltipla, doença neurológica crônica, de evolução contínua e diferenciada, demanda autoconhecimento do corpo, para melhor percepção das capacidades preservadas, das perdas gradativas e da repercussão na realização das atividades e na participação social. Objetivo: Analisar a experiência grupal de aplicação das técnicas corporais baseadas no método self-healing, para a promoção da saúde e reabilitação de pessoas com esclerose múltipla, desenvolvida pela terapia ocupacional. Método: Pesquisa qualitativa do tipo documental referente aos registros escritos e áudios transcritos das sessões grupais. Para análise dos dados foi utilizado o método do Discurso do Sujeito Coletivo. Resultados: Participaram do grupo terapêutico 10 adultos com esclerose múltipla, com idades e tempos de doença variados. Foram identificados cinco discursos relacionados às representações e experiências decorrentes da doença e dos efeitos do aprendizado das técnicas corporais do método self-healing. Como benefícios destacam-se maior percepção corporal, diminuição dos sintomas, melhora da capacidade funcional e reconhecimento da necessidade da prática corporal na rotina. Conclusão: O uso terapêutico do método self-healing demonstrou sua plicabilidade para a promoção da saúde e reabilitação, em conformidade com as políticas da saúde. Dada a pouca literatura existente sobre os benefícios do uso do método self-healing indica-se o desenvolvimento de novos estudos.

Introduction: Multiple sclerosis is a neurological chronic disease with continuous and differentiated evolution, it demands body self-knowledge for better understanding of preserved capacities, gradual losses and repercussion in the performance of activities and social participation. Objective: To analyze the group experience of the application of physical techniques based on self-healing method for health promotion and rehabilitation of people with multiple sclerosis, developed by Occupational Therapy. Method: Documental qualitative research referring to written records and audio transcripts of group sessions. Data analysis used the Collective Subject Discourse method. Results: Ten adults with multiple sclerosis, with varying ages and disease times, participated in the therapeutic group. Five participants reported representations and experiences due to the disease and the effect of learning the physical techniques of self-sealing. The benefits include a greater body awareness, decreased symptoms, improved functional capacity and recognition of the need of body practice routine. Conclusion: The therapeutic use of self-healing method demonstrated its applicability to promote the health benefits, rehabilitation, according to health policies. Due to limited literature on the benefits of using the self-healing method indicates the development of new studies.

Desenvolvimento de manual para orientações básicas do dia a dia para pessoas com esclerose múltipla / Manual development for basic guidelines of the day-to-day for people with multiple sclerosis

Objetivo: O estudo visa apresentar as etapas desenvolvidas para o aprimoramento de manual de orientação e sua aplicação com pessoas com esclerose múltipla (EM), ocorrida em um grupo terapêutico conduzido pela Terapia Ocupacional. Conforme as políticas de saúde, a produção de materiais educativos tem caráter multidisciplinar e é útil como ferramenta para a promoção de saúde devido à sua capacidade de aumentar o conhecimento e de solucionar problemas. O caráter crônico e progressivo da EM traz alterações em diferentes funções do corpo e demanda mudanças e estratégias para minimizar o seu impacto na vida das pessoas. Método: Estudo documental baseado nos registros escritos e áudios produzidos nos encontros grupais no período de 2013 a 2014. A análise dos documentos foi feita com base no método do Discurso do Sujeito Coletivo (DSC). Resultados: Participaram do estudo 10 pessoas com EM, com idade entre 23 a 55 anos, a maioria com mais de cinco anos de doença. Foram gerados quatro DSCs. De acordo com os discursos, o manual serviu não só como uma ferramenta prática e de fácil consulta, o que favoreceu a desmistificação da doença, mas também como guia para as atividades da rotina, apoio para identificação dos problemas e facilitação na convivência com a rede social. Conclusão: O uso do manual mostrou-se útil por facilitar a prática do aprendizado adquirido no grupo e estimular a corresponsabilização com a própria saúde.

Objective: The study aims to present the steps developed for the guidance manual enhancement and its application on people with multiple sclerosis occurred in a therapeutic group conducted by the Occupational Therapy. According to health policies, the production of educational materials is multidisciplinary and is useful as a tool to promote health due to this ability to increase the knowledge and problem solutions. The chronic and progressive disease brings alterations in different body functions and demands changes and strategies to minimize their impact on people's lives. Method: Documentary study based on written records and audio produced in the group meetings in the period from 2013 to 2014. The analysis of the documents was based on the Collective Subject Discourse (CSD) method. Results: The study included 10 people with multiple sclerosis, aged 23-55 years, the majority with over 5 years of disease. It was generated four CSD. According to the speeches the manual served as a practical tool and easy consultation, which favored demystify the disease, served as a guide for the routine activities, support identifying problems and facilitating the interaction with the social network. Conclusion: The use of the manual was useful because it facilitates the practice of acquired skills in the group and encourage co-responsibility for their own health.

Biosynthesis of flavonoids in achenes of Fragaria chiloensis ssp. chiloensis / Biosintesis de flavonoides en aquenios de Fragaria chiloensis ssp. chiloensis

Fragaria chiloensis ssp. chiloensis has two native botanical forms. Fruits from both botanical forms, Fragaria chiloensis ssp. chiloensis f. chiloensis (native white strawberry) and f. patagonica (native red strawberry), were collected from Bio-Bio Region, and a comparative study in the biosynthesis and pigment accumulation was performed from achens. The fruit was classified in four different developmental and ripening stages in order to establish the differences in the transcriptional profile of structural genes and the chemical compounds. A differential expression of those genes involved in the biosynthesis (phenylpropanoid and flavonoids) of anthocianins was found. The differential expression of genes was concomitant with the increase in the level of cyanidin 3-glucoside (C3G) along the fruit development for both botanical forms. On the contrary, undetectable level of cyanidin 3-glucoside (P3G) was observed in the f. chiloensis. Albeit, P3G increase rapidly from the development stage 2, reaching the maximum value at stage 4 in Fragaria chiloensis ssp. chiloensis f. patagonica.

Fragaria chiloensis ssp. chiloensis presenta dos formas botánicas nativas. Los frutos de ambas formas botánicas, Fragaria chiloensis ssp. chiloensis f. chiloensis (frutilla nativa blanca) y f. patagonica (frutilla nativa roja), fueron colectadas en la región del Bio-Bio, realizándose un estudio comparativo en la biosíntesis y acumulación de la pigmentación en aquenios. Para ello, el fruto fue clasificado en cuatro distintos estadios de desarrollo y maduración a fin de establecer las diferencias en los perfiles transcripcionales de genes estructurales y de compuestos químicos. Se determinó una expresión diferencial de los genes responsables de la formación de antocianinas, concomitante con un incremento en los niveles de cianidina 3-glucósido (C3G) en tanto avanza el desarrollo del fruto en ambas formas botánicas. Por el contrario, se observó niveles indetectables de pelargonidina 3-glucósido (P3G) en f. chiloensis, lo cual contrasta con lo observado en f. patagonica, donde P3G se incrementa rápidamente a partir del estadio 2, alcanzando un máximo valor en estadio 4.

Increased accumulation of anthocyanins in Fragaria chiloensis fruits by transient suppression of FcMYB1 gene.

Anthocyanins and proanthocyanidins (PAs), flavonoid-derived metabolites with different physiological roles, are produced by plants in a coordinated manner during fruit development by the action of transcription factors (TFs). These regulatory proteins have either an activating or repressing effect over structural genes from the biosynthetic pathway under their control. FaMYB1, a TF belonging to the R2R3-MYB family and isolated from commercial strawberry fruit (Fragaria×ananassa), was reported as a transcriptional repressor and its heterologous over-expression in tobacco flowers suppressed flavonoid-derived compound accumulation. FcMYB1, an ortholog of FaMYB1 isolated from the white Chilean strawberry (Fragaria chiloensis ssp. chiloensis f. chiloensis), showed higher transcript levels in white (F. chiloensis) than in red (F.×ananassa cv. Camarosa) fruits. In order to assess its contribution to the discolored phenotype in F. chiloensis, FcMYB1 was transiently down-regulated in planta using an RNAi-based approach. Quantitative real-time PCR on FcMYB1 down-regulated fruits resulted an up-regulation of anthocyanidin synthase (ANS) and a strong repression of anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) transcript accumulation. In addition, these fruits showed increased concentrations of anthocyanins and undetectable levels of flavan 3-ols. Altogether, these results indicate a role for FcMYB1 in regulation of the branching-point of the anthocyanin/PA biosynthesis determining the discolored phenotype of the white Chilean strawberry fruit.

Comparison of transcriptional profiles of flavonoid genes and anthocyanin contents during fruit development of two botanical forms of Fragaria chiloensis ssp. chiloensis.

Difference in fruit pigmentation observed between two botanical forms of Fragaria chiloensis ssp. chiloensis (form chiloensis and form patagonica) was studied through transcriptional and chemical approaches. The proportion of different anthocyanins was demonstrated to be characteristic of each botanical form, with pelargonidin 3-glucoside being the most abundant in f. patagonica fruit and cyaniding 3-glucoside as the major one in f. chiloensis fruit. Partial gene sequences of the phenylpropanoid and flavonoid biosynthesis pathways were isolated from the native Chilean strawberry fruits, and used to design gene-specific primers in order to perform transcriptional analyses by qRT-PCR. These genes showed spatial, developmental, and genotypic associated patterns. The red fruit of f. patagonica exhibited higher transcript levels of anthocyanin-related genes and higher levels of anthocyanins compared to the barely pigmented fruit of f. chiloensis. The anthocyanin accumulation in F. chiloensis ssp. chiloensis fruits was concomitant with the particular progress of the transcriptional activity of genes involved in the biosynthesis of flavonoid pigments. The differences in anthocyanin contents, both in terms of type and quantity, between the two botanical forms of F. chiloensis ssp. chiloensis were coincident with the differential transcriptional patterns found in the anthocyanin-related genes.

Isolation of genes differentially expressed during development and ripening of Fragaria chiloensis fruit by suppression subtractive hybridization.

Fragaria chiloensis, the native Chilean strawberry, is noted for its good fruit quality characters. However, it is a highly perishable fruit due to its rapid softening. With the aim to screen for genes differentially expressed during development and ripening of strawberry fruit, the subtractive suppressive hybridization (SSH) methodology was employed. Six libraries were generated contrasting transcripts from four different developmental stages. A set of 1807 genes was isolated and characterized. In our EST collection, approximately 90% of partial cDNAs showed significant similarity to proteins with known or unknown function registered in databases. Among them, proteins related to protein fate were identified in a large green fruit library and protein related with cellular transport, cell wall-related proteins, and transcription regulators were identified in a ripe fruit library. Thirteen genes were analyzed by qRT-PCR during development and ripening of the Chilean strawberry fruit. The information generated in this study provides new clues to aid the understanding of the ripening process in F. chiloensis fruit.

Leaf movements and photoinhibition in relation to water stress in field-grown beans.

Photoinhibition in plants depends on the extent of light energy being absorbed in excess of what can be used in photochemistry and is expected to increase as environmental constraints limit CO2 assimilation. Water stress induces the closure of stomata, limiting carbon availability at the carboxylation sites in the chloroplasts and, therefore, resulting in an excessive excitation of the photosynthetic apparatus, particularly photosystem II (PSII). Mechanisms have evolved in plants in order to protect against photoinhibition, such as non-photochemical energy dissipation, chlorophyll concentration changes, chloroplast movements, increases in the capacity for scavenging the active oxygen species, and leaf movement or paraheliotropism, avoiding direct exposure to sun. In beans (Phaseolus vulgaris L.), paraheliotropism seems to be an important feature of the plant to avoid photoinhibition. The extent of the leaf movement is increased as the water potential drops, reducing light interception and maintaining a high proportion of open PSII reaction centres. Photoinhibition in water-stressed beans, measured as the capacity to recover F(v)/F(m), is not higher than in well-watered plants and leaf temperature is maintained below the ambient, despite the closure of stomata. Bean leaves restrained from moving, increase leaf temperature and reduce qP, the content of D1 protein and the capacity to recover F(v)/F(m) after dark adaptation, the extent of such changes being higher in water-stressed plants. Data are presented suggesting that even though protective under water stress, paraheliotropism, by reducing light interception, affects the capacity to maintain high CO2 assimilation rates throughout the day in well-watered plants.