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1.
PLoS One ; 14(12): e0225736, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31830056

RESUMO

The objective of this study was to evaluate the biological effects of roasted Cashew nuts consumption on biochemical and murinometric parameters in dyslipidemic rats receiving lipid supplementation. Young male rats were randomly assigned to three experimental groups (n = 10). The Control group (CONT) was treated with water, the Dyslipidemic group (DL) received a high fat content emulsion throughout the experiment, and the Dyslipidemic Cashew Nuts group (DLCN) received the same high fat content emulsion throughout the experiment, yet was treated with Cashew nuts. Body parameters, biochemical, hepatic and fecal fatty acid profiles were all evaluated. The levels of total cholesterol and triglycerides were higher in the DL and DLCN groups as compared to the control group. DLCN and CONT presented no difference in HDL levels. DLCN presented higher glycemia levels than the other groups. There was reduction of body fat in DLCN as compared to other groups, but with higher accumulations of liver fat. DLCN presented a reduction in saturated hepatic fatty acids of 20.8%, and an increase of 177% in relation to CONT; there was also a 21% in increase DL for ω9 fatty acids in comparison to CONT. As for fecal fatty acids, there was a lower concentration of polysaturates in DLCN as compared to the other groups. The data showed that the consumption of Cashew nuts by the dyslipidemic animals treated with a hyperlipidic diet induced greater accumulations of liver fat and worsened glycemic levels, despite having reduced visceral fats and increased fecal fat excretion.


Assuntos
Anacardium/química , Glicemia/metabolismo , Dislipidemias/sangue , Gordura Intra-Abdominal/efeitos dos fármacos , Extratos Vegetais/farmacologia , Adiposidade/efeitos dos fármacos , Animais , Área Sob a Curva , Peso Corporal , Ingestão de Energia , Ácidos Graxos/metabolismo , Fezes/química , Comportamento Alimentar , Teste de Tolerância a Glucose , Fígado/efeitos dos fármacos , Fígado/patologia , Masculino , Tamanho do Órgão/efeitos dos fármacos , Fenóis/análise , Ratos Wistar
2.
Int J Biol Macromol ; 139: 57-62, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31356940

RESUMO

The present research aimed investigates the characterization and concentration of oligosaccharides naturally present in goat cheese whey obtained from two types of goat milk. The goat cheese whey was processed by a two-step cross-flow filtration process and a hydrophilic interaction chromatography - Ultra-Performance Liquid Chromatography coupled to a High Definition Mass Spectrometry. A Quadrupole Time-of-Flight (HILIC UPLC-HDMS-Q-TOF) method was used to identify and measure five different oligosaccharides in the samples. A final product with recovery of 63-96% of oligosaccharides was obtained when compared with the original whey. These components indicate that goat whey can be used as a source of oligosaccharides with potential functional and possible application for human nutrition.


Assuntos
Queijo , Fracionamento Químico/métodos , Cabras , Membranas Artificiais , Oligossacarídeos/química , Oligossacarídeos/isolamento & purificação , Soro do Leite/química , Animais
3.
PLoS One ; 12(9): e0185382, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28957373

RESUMO

This study evaluated the intestinal anti-inflammatory effects of goat whey in a mouse model of colitis induced by 2,4-dinitrobenzenesulfonic acid that resembles human IBD. At a concentration of 4 g/kg/day, the goat whey improved the symptoms of intestinal inflammation, namely by decreasing the disease activity index, colonic weight/length, and leukocyte infiltration. Moreover, goat whey inhibited NF-κB p65 and p38 MAPK signaling pathways and consequently down-regulated the gene expression of various proinflammatory markers such as IL-1ß, IL-6, IL-17, TNF-α, iNOS, MMP-9, ICAM-1. Also, goat whey increased the expression of proteins such as mucins, occludin proteins and cytokine signalling suppressors. The immunomodulatory properties of goat whey were also evaluated in vitro using the murine macrophage cell line Raw 264 and CMT-93 cells derived from mouse rectum carcinomas. The results revealed the ability of goat whey to inhibit the production of NO and reduce IL-6 production in LPS-stimulated cells. In conclusion, goat whey exhibited anti-inflammatory effects in the DNBS model of intestinal inflammation, and these observations were confirmed by its immunomodulatory properties in vitro. Together, our results indicate that goat whey could have applications for the treatment of IBD.


Assuntos
Anti-Inflamatórios/uso terapêutico , Colite/induzido quimicamente , Colite/patologia , Intestinos/patologia , Soro do Leite/química , Animais , Anti-Inflamatórios/farmacologia , Colite/genética , Citocinas/genética , Citocinas/metabolismo , Dinitrofluorbenzeno/análogos & derivados , Modelos Animais de Doenças , Ensaio de Imunoadsorção Enzimática , Imunofluorescência , Regulação da Expressão Gênica/efeitos dos fármacos , Cabras , Mediadores da Inflamação/metabolismo , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/patologia , Intestinos/efeitos dos fármacos , Masculino , Camundongos , Células RAW 264.7 , Reação em Cadeia da Polimerase em Tempo Real
4.
Food Chem ; 225: 202-212, 2017 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-28193416

RESUMO

The need to increase sustainability and add value to traditional foods claiming health benefits led to the introduction of key improvements in the production of hibiscus beverages in Senegal. The physicochemical and sensory properties of three resulting products (an under-vacuum concentrate, a dilute-to-taste syrup and a ready-to-drink infusion) were assessed, vis-à-vis those of conventionally manufactured beverages, and their impact on local consumer preference determined (n=146). New beverages had more intense, redder colour and higher monomeric anthocyanin content, total phenolic content and antioxidant capacity. Moreover, their colour evaluations by trained panellists were mainly linked to colour density and anthocyanin/polyphenol content, while flavour assessments were associated to titratable acidity and sugar-to-acid ratio. Consumer evaluations, in turn, were driven by the beverages' red colour intensity, aroma strength and balance between sweetness and acidity. This explained why they overwhelmingly preferred the under-vacuum concentrate, regardless of their age, gender or frequency of hibiscus beverage consumption.


Assuntos
Hibiscus/química , Extratos Vegetais/química , Antioxidantes , Comportamento do Consumidor , Feminino , Humanos , Masculino , Polifenóis
5.
Food Funct ; 7(9): 4020-4029, 2016 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-27711901

RESUMO

Goat meat is extensively known for its interesting nutritional value and for being an important source of protein with high quality. Its food derivatives are, therefore, a good alternative to develop new products addressed to health conscious consumers. In this work, a healthier goat product, namely, a low fat fresh sausage, was produced with the objective of evaluating the effect of inclusion of chitosan on quality, stability and shelf life. Sausages containing 2% chitosan were formulated with different fat levels (5%, 12.5% and 20%, w/w) and stored at 4 °C for 15 days. Results indicated the incorporation of 2% (w/w) chitosan was technologically feasible, due to the reduction of microbial growth and lipid oxidation, as well as the enhancement of red color. Additionally, the treated samples improved all the characteristics associated with cooking, showing the ability to bind water and fat and acquiring a firmer texture compared with control samples. Additionally, the reduction of fat content is technologically feasible without negative influences on the final product.


Assuntos
Quitosana/química , Culinária , Dieta com Restrição de Gorduras , Gorduras na Dieta/análise , Conservantes de Alimentos/química , Qualidade dos Alimentos , Produtos da Carne/análise , Animais , Fenômenos Químicos , Dieta com Restrição de Gorduras/etnologia , Enterobacteriaceae/crescimento & desenvolvimento , Enterobacteriaceae/isolamento & purificação , Estudos de Viabilidade , Armazenamento de Alimentos , Fungos/crescimento & desenvolvimento , Fungos/isolamento & purificação , Cabras , Dureza , Produtos da Carne/microbiologia , Oxirredução , Pigmentos Biológicos/análise , Pigmentos Biológicos/química , Portugal , Análise de Componente Principal , Refrigeração , Leveduras/crescimento & desenvolvimento , Leveduras/isolamento & purificação
6.
Food Funct ; 6(8): 2768-78, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26158872

RESUMO

A low fat fresh pork sausage based on chitosan was developed with the objective of obtaining a new functional meat product with improved properties and health claims promoting cholesterol reduction. Sausages were formulated with chitosan (2%, w/w) and different fat levels (5%, 12.5% and 20%, w/w). The results indicated that incorporation of 2% chitosan into produced pork sausages with health claims of reduction of cholesterol is technologically feasible. In addition, the chitosan reduced the microbial growth, revealing interesting fat and water absorption capacities, reduced lipid oxidation, provided greater stability in terms of colorimetric parameters and promoted positive firmer texture and gumminess. The reduction of fat content to levels of 5% was positively achieved with the incorporation of chitosan. Sensorial analysis showed that panelists did not detect any significant difference in taste and any unfavorable effect on the sausage appearance as a consequence of chitosan addition and variation of fat.


Assuntos
Quitosana/análise , Gorduras na Dieta/análise , Aditivos Alimentares/análise , Manipulação de Alimentos/métodos , Produtos da Carne/análise , Animais , Armazenamento de Alimentos , Humanos , Suínos , Paladar
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