In vivo human medial gastrocnemius fascicle behaviour and belly gear during submaximal eccentric contractions are not affected by concentric fatiguing exercise.

Changes in muscle geometry and belly gearing during eccentric contractions influence fibre strain and susceptibility to muscle damage. They are modulated by the interaction between connective tissues and intracellular-intrafascicular fluid pressures and external pressures from neighbouring structures. Fatiguing exercise triggers fluid shifts (muscle swelling) and muscle activation changes that may influence these modulators. Our purpose was to measure medial gastrocnemius (MG) geometric changes in vivo during eccentric contractions before and after maximal concentric muscle work to test the hypothesis that fatigue would reduce fascicle rotation and muscle gear and provoke greater fascicle strain. Submaximal eccentric plantar flexor contractions at 40% and 60% of maximal eccentric torque were performed on an isokinetic dynamometer at 5°.s-1 before and immediately after the fatiguing exercise. MG fascicles and muscle-tendon junction were captured using ultrasonography during contractions, allowing quantification of geometric changes, whole-MG length, and belly gear (Δmuscle length/Δfascicle length). Triceps surae (TS) activation was estimated using surface electromyography and the distribution of activations between synergistic muscles was then determined. After exercise, concentric torque decreased ∼39% and resting muscle thickness increased by 4%, indicating muscle fatigue and swelling, respectively. While soleus (Sol) activation and the Sol/TS ratio increased, no changes in MG, MG/TS ratio or fascicle rotation during the contraction were detected. Thus, fascicle lengthening and belly gear remained unaltered. Changes in muscle thickness during contraction was also similar before and after exercise, suggesting that changes in muscle shape were relatively unaffected by the exercise. Consequently, the muscle maintained mechanical integrity after the fatiguing muscle work.

Hamstring-to-quadriceps activation ratio during lower-limb strengthening exercises.

Multiple exercises included in strength training involve greater activation of the quadriceps compared to hamstring muscles, which may lead to knee joint imbalances. The aim of this study was to examine the ratio of surface electromyography (sEMG) activity hamstring and quadriceps muscle groups (hamstring-to-quadriceps activation ratio; H:Q EMG), as well as lateral to medial hamstring activation ratio (LH:MH) in parallel squat, Romanian deadlift, hip thrust, lying leg curl and seated knee extension. The H:Q EMG was greater during lying leg curl compared to other exercises during both the concentric and eccentric phase, however the Romanian deadlift and hip thrust also resulted in relatively high H:Q EMG. Pairwise comparisons revealed that LH:MH was greater in the parallel squat compared to the Romanian deadlift and hip thrust in the concentric phase, and compared to the Romanian deadlift and hip thrust during the eccentric phase. Our data suggests that the lying leg curl promotes the greatest hamstring activation and H:Q EMG, amongst the exercises investigated, while also providing relatively homogenous LH:MH. The lying leg curl should be considered as a primary exercise in rehabilitation and training programmes, aiming to proportionally activate LH:MH and increase H:Q EMG, which may improve knee muscle balance.

Human in vivo medial gastrocnemius gear during active and passive muscle lengthening: effect of inconsistent methods and nomenclature on data interpretation.

'Muscle gear' is calculated as the ratio of fascicle-to-muscle length change, strain, or velocity. Inconsistencies in nomenclature and definitions of gear exist across disciplines partly due to differences in fascicle [curved (Lf) versus linear (Lf,straight)] and muscle [whole-muscle belly (Lb) versus belly segment (Lb,segment)] length calculation methods. We tested whether these differences affect gear magnitude during passive and active muscle lengthening of human medial gastrocnemius of young men (n=13, 26.3±5.0 years) using an isokinetic dynamometer. Lb, Lb,segment, Lf and Lf,straight were measured from motion analysis and ultrasound imaging data. Downshifts in belly gear but not belly segment gear occurred with muscle lengthening only during active lengthening. Muscle gear was unaffected by fascicle length measurement method (P=0.18) but differed when calculated as changes in Lb or Lb,segment (P<0.01) in a length-dependent manner. Caution is therefore advised for the use and interpretation of different muscle gear calculation methods and nomenclatures in animal and human comparative physiology.

Low Pre-Season Hamstring-to-Quadriceps Strength Ratio Identified in Players Who Further Sustained In-Season Hamstring Strain Injuries: A Retrospective Study from a Brazilian Serie A Team.

A common pre-season injury prevention assessment conducted by professional football clubs is the hamstring-to-quadriceps (H:Q) strength ratio calculated by peak torque (PT). However, it is debatable whether players that present low pre-season H:Q ratios are more susceptible to further sustaining in-season hamstring strain injuries (HSI). Based upon retrospective data from a Brazilian Serie A football squad, a particular season came to our attention as ten out of seventeen (~59%) professional male football players sustained HSI. Therefore, we examined the pre-season H:Q ratios of these players. H:Q conventional (CR) and functional (FR) ratios, and the respective knee extensor/flexor PT from the limbs of players further sustaining in-season HSI (injured players, IP) were compared to the proportional number of dominant/non-dominant limbs from uninjured players (UP) in the squad. FR and CR were ~18-22% lower (p < 0.01), whereas quadriceps concentric PT was ~25% greater for IP than UP (p = 0.002). Low scores of FR and CR were correlated (p < 0.01) with high levels of quadriceps concentric PT (r = -0.66 to -0.77). In conclusion, players who sustained in-season HSI had lower pre-season FR and CR compared to UP, which appears to be associated with higher levels of quadriceps concentric torque than hamstring concentric or eccentric torque.

Do Intramuscular Temperature and Fascicle Angle Affect Ultrasound Echo Intensity Values?

PURPOSE: Ultrasound-derived echo intensity (EI) has been used as a physiological marker for changes in skeletal muscle "quality" with physical training, disuse, aging, and neuromuscular disorders. However, the methodological and physiological factors influencing EI and its longitudinal change are still unclear. Here, we performed two separate experiments to investigate the effects of muscle temperature and fascicle angle, which are known to influence muscle tissue and sound wave properties and therefore affect EI. METHODS: In experiment 1 ( n = 16, 28.0 ± 6.6 yr), vastus lateralis (VL) ultrasonographic images were acquired and intramuscular temperature continuously recorded for 15 min after 20 min of heating to 40.4°C ± 0.7°C using a microwave device. In experiment 2 ( n = 17, 30.2 ± 9.8 yr), VL sonographic images were obtained with the knee both fully extended (0°) and flexed to 90° and EI and fascicle angle measured post hoc . Fascicle movement was tracked during the passive knee flexion to ensure that sonographic images were obtained at the same muscle region. Knee flexion reduced muscle thickness, and we therefore reran analyses calculating EI using identical dimensions to minimize this effect. RESULTS: EI decreased only immediately after the passive heating, and although a moderate, negative correlation was observed between EI and temperature ( rrm = -0.36), the effect of muscle temperature was small ( ß = 0.97 (-1.89 to -0.06) per degree Celsius, P = 0.051). Nonetheless, EI increased as fascicle angle decreased, and a large, negative correlation ( rrm = -0.85) was observed; the effect of fascicle angle on EI was large ( ß = 3.0 (-3.8 to -2.2) per degree, P < 0.01), and this was maintained when analyses were performed at a constant depth of the region of interest ( ß = 3.5 (-4.4 to -2.7) per degree, P < 0.01). CONCLUSIONS: These findings support the hypothesis that fascicle angle meaningfully affects VL EI but provides weak evidence of a temperature effect in vivo . Thus, acute fascicle angle alterations should be accounted for in studies using EI measurements, and longer-term studies should consider whether changes in EI might be partly explained by a change in fascicle angle.

Moving forward with the echo intensity mean analysis: Exploring echo intensity bands in different age groups.

This study compared ultrasound characteristics in youth, younger adults, and older adults using two different methods of analysis: based on clusters of pixel concentration of the grayscale (i.e. EI bands) and the traditionally calculated echo intensity mean. Forty-four healthy youth (13.3 ± 1.4 y), 22 younger adults (31.8 ± 10.1 y) and 53 older adults (66.7 ± 4.6 y) volunteered for the assessment of rectus femoris EI via ultrasonography. Pixel concentration (i.e. EI bands) was calculated in intervals of 0-50, 51-100, 101-150, 151-200, and 201-255 of the gray scale, while EI mean was determined as the average pixels from 0 to 255. EI0-50 significantly decreased with group age, while EI51-100, 101-150, 151-200, 201-255 and EI mean increased (P < 0.001). The differences between groups were greater using EI0-50 compared to the EI mean (i.e. EI0-50: youth = ~353% and younger adults = ~251% > older adults; whereas EI mean: youth = ~46% and younger adults = 29% < older adults). These results potentially indicate that EI bands method offer different information than the EI mean method. In addition, EI bands may be a promising approach to understand tissue-specific adaptations to physical training and neuromuscular diseases, yet future studies should validate this method.

The effect of isokinetic dynamometer deceleration phase on maximum ankle joint range of motion and plantar flexor mechanical properties tested at different angular velocities.

During range of motion (max-ROM) tests performed on an isokinetic dynamometer, the mechanical delay between the button press (by the participant to signal their max-ROM) and the stopping of joint rotation resulting from system inertia induces errors in both max-ROM and maximum passive joint moment. The present study aimed to quantify these errors by comparing data when max-ROM was obtained from the joint position data, as usual (max-ROMPOS), to data where max-ROM was defined as the first point of dynamometer arm deceleration (max-ROMACC). Fifteen participants performed isokinetic ankle joint max-ROM tests at 5, 30 and 60° s-1. Max-ROM, peak passive joint moment, end-range musculo-articular (MAC) stiffness and area under the joint moment-position curve were calculated. Greater max-ROM was observed in max-ROMPOS than max-ROMACC (P < 0.01) at 5 (0.2 ±â€¯0.15%), 30 (1.8 ±â€¯1.0%) and 60° s-1 (5.9 ±â€¯2.3%), with the greatest error at the fastest velocity. Peak passive moment was greater and end-range MAC stiffness lower in max-ROMPOS than in max-ROMACC only at 60° s-1 (P < 0.01), whilst greater elastic energy storage was found at all velocities. Max-ROM and peak passive moment are affected by the delay between button press and eventual stopping of joint rotation in an angular velocity-dependent manner. This affects other variables calculated from the data. When high data accuracy is required, especially at fast joint rotation velocities (≥30° s-1), max-ROM (and associated measures calculated from joint moment data) should be taken at the point of first change in acceleration rather than at the dynamometer's ultimate joint position.

Hamstring rate of torque development is more affected than maximal voluntary contraction after a professional soccer match.

Match-induced fatigue of knee muscle strength and agonist-antagonist strength-ratios may affect both performance and risk of injury in soccer players. Once explosive tasks are imperative in soccer as well as hamstring strain injuries occur during high-velocity moments, rapid force capacity of this muscle group is especially important. This study evaluated the effect of match-induced fatigue on knee muscle strength and strength-ratio parameters after a single professional soccer match. Male professional soccer players (n = 16; 24.2 ± 3.9 years) were tested before and after a soccer match (56.2 ± 22.6 min of playing) for knee flexors (hamstring) and extensors (quadriceps) isometric peak torque (MVC) and rate of torque development (RTD) - as well as the hamstring-to-quadriceps ratio (H:Q) - at 30° of knee flexion. Knee injuries often occur at this joint angle, which is common in sprinting, pivoting, sidecutting, and jumping. Match-induced fatigue caused a left shift in the knee extensors torque-time curve with no significant change in both early (i.e. 0-50 ms) and late (i.e. 0-200 ms) RTD, and a right shift in the knee flexors torque-time curve with a decrease in early RTD (∼16%, p = .029) and late RTD (∼11%, p = .011). Knee extensors and knee flexors peak torque remained unchanged (p > .05). Early RTD H:Q decreased by∼24% (p = .027), while late RTD H:Q and MVC H:Q remained unchanged (p > .05). In conclusion, match-induced fatigue impaired the ability to rapidly produce force at an angle where injuries are most susceptible to occur. Important information is missed if only the traditional H:Q is considered.

Does Rest Time before Ultrasonography Imaging Affect Quadriceps Femoris Muscle Thickness, Cross-Sectional Area and Echo Intensity Measurements?

In the work described here, our aim was to determine, in an elderly population, changes in muscle thickness (MT), cross-sectional area (CSA) and echo intensity (EI) of the quadriceps muscles at four time points (0, 5, 10 and 15 min; i.e., T0, T5, T10 and T15, respectively) after changing from a standing to supine position. Twenty-one elderly participants (14 men: 68.1 ± 4.6 y; 8 women: 66.8 ± 4.1 y) were evaluated at four time points. Rectus femoris CSA (RFCSA), MT and EI of the quadriceps femoris (QF) muscles were assessed. EI significantly increased from T0 to T5, T10 and T15 (p < 0.001), whereas no differences were observed between T5 and T15 in the rectus femoris (RFEI), vastus intermedius (VIEI) and quadriceps femoris (QFEI). No differences were observed between any time points in the RFCSA and MT of QF muscles. In summary, these results suggest that periods >5 min are not necessary to obtain consistent MT and EI measurements of quadriceps femoris muscles in the elderly population.

Electromyographic evaluation of high-intensity elastic resistance exercises for lower extremity muscles during bed rest.

PURPOSE: Prolonged hospital bed rest after severe injury or disease leads to rapid muscle atrophy and strength loss. Therefore, the main aim of this study was to evaluate the efficacy of lower extremity strengthening exercises using elastic resistance that can be performed while lying in a hospital bed. METHODS: Using a cross-sectional design, 22 healthy individuals performed three consecutive repetitions of 14 different lower extremity exercises using elastic resistance, with a perceived intensity corresponding to 8 on the Borg CR-10 scale. Surface electromyography was measured on 13 lower extremity muscles and normalized to the maximal EMG (nEMG). Likewise, exercise satisfaction was evaluated by a questionnaire. RESULTS: All participants were able to perform all exercises without discomfort and generally rated them satisfactory. High levels of muscle activity were observed for all prime movers. For example, the "femoris muscle setting" exercise showed high levels of muscle activity for rectus femoris, vastus lateralis, and vastus medialis (79, 75, and 79% nEMG, respectively), while biceps femoris and semitendinosus were highly active during the prone knee flexion exercise with (72 and 71% nEMG, respectively) and without Kinesiology Tape (73 and 77% nEMG, respectively). CONCLUSION: High levels of muscle activity in the lower extremities can be achieved using elastic resistance exercises performed when lying in a hospital bed. Even though performed on healthy individuals, the present study has the potential to provide a reference table of exercises to select from when individualizing and progressing strengthening exercises during the early rehabilitation of bedridden individuals.