Lack of correlation between DNA copy number and mRNA expression levels of c-myc in gamma-radiation-induced mouse thymic lymphomas by using quantitative real-time PCR.

BACKGROUND: It is well documented that over-expression of the c-myc proto-oncogene occurs in the vast majority of mouse thymic lymphomas induced by gamma-irradiation, evidencing the importance of this gene in T-cell lymphomagenesis. However, it remains unknown whether elevated levels of c-myc expression are driven by extra c-myc copy numbers. MATERIALS AND METHODS: Here we use a quantitative test on the basis of real-time PCR to determine the cellular copy number of c-myc in a set of 14 g-radiation- induced thymic lymphomas obtained from (C57BL/6J x BALB/cJ) F1 hybrid mice with increased mRNA c-myc expression. RESULTS: Since 5 out of 14 (35.7%) cases had no extra copy numbers of c-myc, gene amplification was obviously not the cause of c-myc over-expression in these tumours. In the remaining 9 tumours, c-myc over-expression was also accompanied with extra DNA copy numbers. Therefore, c-myc amplification might be a consequence of the genomic instability subsequent to the up-regulation of c-myc. However, linear regression analysis showed a lack of correlation between increasing DNA copy numbers and mRNA over expression of c-myc in these tumours (r = 0.029, p = 0.94). CONCLUSION: De-regulation of c-myc does not necessarily imply amplification of this gene in these tumours. This report is, to our knowledge, the first one comparing c-myc amplification with expression in lymphomas of the T-cell lineage.

Prevalencia de marcadores sericos del virus de la hepatitis B en el personal femenino de un hospital general / Prevalence of serum markers of hepatitis B virus in female personnel of a general hospital

Hemos determinado los marcadores del virus de la hepatitis B a 209 mujeres del personal de un hospital general: 12 Médicos, 90 ATS, 70 Auxiliares de clínica y de laboratorio y 37 personas de Personal de limpieza y office. La prevalencia fue del 15,31% (32 personas). Cuatro personas (1,91%) presentaron HBs-Ag. La prevalencia fue más baja que las encontradas por otros autores de nuestro país. Las prevalencias más altas se encontraron en el personal de limpieza y office, seguido de los ATS. Por Servicios, las prevalências más altas se encontraron en el personal en contacto con sangre y hemoderivados. No se encontraron marcadores en el personal de la UVI. La prevalencia aumentó y generalmente con la edad y la antigüedad. Asociamos los bajos índices de prevalencia encontrados por nosotros con los elevados porcentajes de personal joven (33,49%) tienen menos de 30 años y con poca antigüedad en el hospital (26,31% con menos de 4 años

Prevalencia de marcadores sericos del virus de la hepatitis B en el personal femenino de un hospital general / Prevalence of serum markers of hepatitis B virus in female personnel of a general hospital

Hemos determinado los marcadores del virus de la hepatitis B a 209 mujeres del personal de un hospital general: 12 Médicos, 90 ATS, 70 Auxiliares de clínica y de laboratorio y 37 personas de Personal de limpieza y office. La prevalencia fue del 15,31% (32 personas). Cuatro personas (1,91%) presentaron HBs-Ag. La prevalencia fue más baja que las encontradas por otros autores de nuestro país. Las prevalencias más altas se encontraron en el personal de limpieza y office, seguido de los ATS. Por Servicios, las prevalÛncias más altas se encontraron en el personal en contacto con sangre y hemoderivados. No se encontraron marcadores en el personal de la UVI. La prevalencia aumentó y generalmente con la edad y la antig³edad. Asociamos los bajos índices de prevalencia encontrados por nosotros con los elevados porcentajes de personal joven (33,49%) tienen menos de 30 años y con poca antig³edad en el hospital (26,31% con menos de 4 años (AU)

[Prevalence of serum markers of hepatitis B virus in the female personnel at a general hospital]. / Prevalencia de marcadores séricos del virus de la hepatitis B en el personal femenino de un hospital general.

We have determined hepatitis B virus markers to 208 female personnel of a general hospital: 12 physicians 90 nursing personnel, 70 assistants of clinic and laboratory and 37 cleanliness, and office personnel. The prevalence was 15.31% (31 persons). Four persons (1.91%) presented HBs-Ag. The prevalence was more low that the prevalences found by other authors of our country. The highest prevalences were found in the cleanliness and office personnel, pursued of nursing personnel. Among different services, the highest prevalences were found in the personnel in contact with blood and its derivates. Not were found markers in the intensive care unit personnel. The prevalence increased generally with age and with years in occupation. We associated the low young personnel (33.49% are more low of 30 years age) and with short years occupation in the hospital (26.31% are more low of 4 years).

Prevalencia de marcadores séricos del virus de la hepatitis B en el personal femenino de un hospital general. / [Prevalence of serum markers of hepatitis B virus in the female personnel at a general hospital]

We have determined hepatitis B virus markers to 208 female personnel of a general hospital: 12 physicians 90 nursing personnel, 70 assistants of clinic and laboratory and 37 cleanliness, and office personnel. The prevalence was 15.31

(31 persons). Four persons (1.91

) presented HBs-Ag. The prevalence was more low that the prevalences found by other authors of our country. The highest prevalences were found in the cleanliness and office personnel, pursued of nursing personnel. Among different services, the highest prevalences were found in the personnel in contact with blood and its derivates. Not were found markers in the intensive care unit personnel. The prevalence increased generally with age and with years in occupation. We associated the low young personnel (33.49

are more low of 30 years age) and with short years occupation in the hospital (26.31

are more low of 4 years).