RESUMO
Enhancement of cell adhesion and growth on surface of the biodegradable materials is one of the important tasks in development of materials for regenerative medicine. This work focuses on comparison of various methods of collagen coating deposition onto polylactide films, aiming to increase their biocompatibility with human mesenchymal stromal cells. The collagen deposition was realized using either preliminary plasma treatment of the polylactide films or pre-swelling in solvent mixture. These techniques were compared in terms of the effect on the surface's chemical structure, morphology, hydrophilicity and ability to support adhesion and growth of human mesenchymal stromal cells.
RESUMO
Plasma treatment is one of the most promising tools to control surface properties of materials tailored for biomedical application. Among a variety of processing conditions, such as the nature of the working gas and time of treatment, discharge type is rarely studied, because it is mainly fixed by equipment used. This study aimed to investigate the effect of discharge type (direct vs. alternated current) using air as the working gas on plasma treatment of poly(ethylene terephthalate) films, in terms of their surface chemical structure, morphology and properties using X-ray photoelectron spectroscopy, scanning electron microscopy, atomic force microscopy and contact angle measurements. The effect of the observed changes in terms of subsequent chitosan immobilization on plasma-treated films was also evaluated. The ability of native, plasma-treated and chitosan-coated films to support adhesion and growth of mesenchymal stem cells was studied to determine the practicability of this approach for the biomedical application of poly(ethylene terephthalate) films.
