[Fatty acid composition of cells and lipopolysaccliarides of Mannheimia haemolytica, Mannheimia glucosida and Bibersteinia trehalosi strains].

The Mannheimia haemolytica, Mannheimia glucosida and Bibersteinia trehalosi strains and the similar fatty acid composition of cells with domination of C(16:1) and C(16:0), which were in almost equal quantities, C(14:0 and C(18:1) + C(18:2). The fatty acid composition of lipopolysaccharides (LPS) of the studied bacteria had no essential differences too. It was mainly represented by C(14:0) and 3-OH-C(14:0) which consisted of more than 80% of all LPS fatty acids. C(12:0), C(16:1) and C(16:0) were presented in LPS in small quantities. The M. haemolytica, M. glucosida and B. trehalosi strains did not differ essentially by fatty acid compositions of cells and LPS from earlier studied strains of genera Pasteurella (P. multocida), Haemophilus (H. influenzae and other species), Actinobacillus (A. pleuropneumoniae). This shows the close phylogenetic relationship of the mentioned bacteria and significance of investigated signs as chemotaxonomic markers for differentiation of taxons of the above genus level. The paper is presented in Russian.

[Cellular fatty acid composition of the Burkholderia mallei and Burkholderia pseudomallei strains as generic feature of Burkholderia].

The cellular fatty acid compositions of studied two strains of Burkholderia mallei and a strain of Burkholderia pseudomallei are represented by saturated and monounsaturated straight chain fatty acids with 14-18 carbon atoms, cyclopropane fatty acids C(17 inverted delta) and C(19 inverted delta), and hydroxy acids 3-OH-C14:0, 2-OH-C(16:0), and 3-OH-C(16:0). The strain variation of cyclopropane and unsaturated fatty acid levels was observed. The cellular fatty acid spectra of studied bacteria did not depend essentially on growth medium. The levels of cyclopropane fatty acids increased and those of unsaturated ones decreased with culture age, a tendency to increasing the levels of hydroxy fatty acids was observed too.

[Fatty-acid composition of cells and lipopolysaccharides in different Yersinia species under the conditions of growth at low temperature].

Y. pestis, Y. pseudotuberculosis, Y. enterocolitica, Y. frederiksenii, Y. intermedia, Y. kristensenii and Y. ruckeri grown at 4 degrees C were characterized by fatty acid composition with a high content of C16:1 and C18:1, as well as the proportion of saturated to nonsaturated fatty acids equal to, on the average, 2.0. In Yersinia lipopolysaccharides a relatively high level of C16:1 and C12:0 was observed with the prevalence of 3-OH-C14:0. In the fatty-acid spectra of both cells and lipopolysaccharides no essential difference was noted. Thus, during growth at low temperature differences, earlier detected in the studied Yersinia species grown at 37 degrees C and making it possible to divide 7 Yersinia species into 2 groupes, were completely leveled. These results confirmed the close phylogenetic relationship between the Yersinia species under study and were indicative of more pronounced biological community of Yersinia under the conditions of growth at low temperature.

[Clinico-microbiological observations in specialized neonatology departments]. / Kliniko-mikrobiologicheskie nabliudeniia v spetsializirovannykh neonatologicheskikh otdeleniiakh.

Results of dynamic microbiological control and clinical observation in specialized departments of newborns pathologies and nursing of premature children for 1989-1994 are presented. Criteria of gastrointestinal colonization, clinical and bacteriological indices of the risk of appearance of suppurative-septic infections in newborns, the leading role of gram-negative bacteria and the increasing role of staphylococci in the development of these infections have been established. Sensitivity of the hospital strains of microorganisms isolated from newborns and from the environment to antibacterial preparations has been studied. Their multiple drug resistance and uniformity according to antibiotic program which permits supposing intrahospital origin of these strains has been established.

[The immunomodulating properties of the filtrates of Klebsiella culture broth and the nature of the immunomodulating factor]. / Immunomoduliruiushchie svoistva fil'tratov kul'tural'noi zhidkosti klebsiell i priroda immunomoduliruiushchego faktora.

Influence of broth culture filtrates (BCF) of Klebsiella on delayed type hypersensitivity (DTN) in mice has been studied. BCF of plasmid-containing virulent strains and non-plasmid avirulent strains have been found to suppress DTH to xenogenic splenocytes. Gel filtration data have shown that immunosuppressive factor possesses molecular weight about 180-800 kDa. BCF treated with etanol-ether mixture got to soluble fraction that evidenced for its lipid nature. This factor can be inactivated or reactivated by the treatment with trichloracetic acid, phenol and other chemicals. A new factor is found in reactivated BCF. It induces the ability of E. coli O55 to suppress DTH in mice and looks like Shigella factor. It is supposed that Klebsiella immunosuppressive factors are connected with lipopolysaccharide.

[Use of tonic contracture of strips of frog myocardium to study sodium-calcium exchange in the sarcolemma of myocardiocytes]. / Ispol'zovanie tonicheskoi kontraktury polosok miokarda liagushki dlia izucheniia natrii-kal'tsievogo obmena v sarkolemme kardiomiotsitov.

The mechanism of tonic component of the frog myocardium potassium contraction was studied. The degree of contraction depended on the potassium, sodium and calcium ions concentrations in extracellular medium. The tonic component of hyperpotassium contraction, as well as the additional contractions due to hyposodium and hypercalcium medium in the tonic component phase seems to stem from activation of sodium--calcium exchange. The latter through the sarcolemma of the heart cells was 3Na: 1Ca. The effect of some mono- and bivalent cations on sodium-calcium exchange in respect ot their inhibiting activity was as follows: Cd+2 greater than Sr+2 greater than Ba+2 greater than Mn+2 much greater than Mg+2 greater than Li+ greater than Cs+.

[Laser therapy of cervix dysplasia]. / Lazernaia terapiia displazii sheiki matki.

Irradiation from a continuous wave CO2-laser was used in the treatment of 1,234 cases of cervical epithelial dysplasia. Diagnosis was established on the findings of extended colposcopy, aiming biopsy and cytological examination of smears. Such advantages over diathermocoagulation as complication-free recovery and a low incidence of recurrences (2.6%) were shown. The end results were studied.

[Selective action of argon laser radiation in breast cancer]. / Selektivnoe deistvie izlucheniia argonovogo lazera pri rake molochnoi zhelezy.

The attempts were made to apply the argon laser radiation (the green region of light spectrum) for the treatment of breast carcinoma. The 46 post-operative skin metastases of breast carcinoma in 15 women were irradiated with no photosensitizers used. As a result of the treatment (4 to 50 sessions) the complete resolution of metastatic tumours was achieved in 13 patients. In contrast to the methods of laser coagulation treatment of tumours, used till now, the described approach provides for selective elimination of tumour cells without normal tissue alteration. The involvement of photothermic or photochemical mechanisms in this selective effect is discussed.

[Treatment of skin tumors with a pulse laser]. / Lechenie opukholei kozhi impul'snym lazerom.

The clinical laser complex including neodymium and CO2--lasers is described, and the methods of irradiation depending on the type of the laser and tumor are given. The results of the treatment in 559 patients with different malignant and benign skin tumors are analysed. The data of the follow-up (up to 7 years) indicated that 15 of 48 patients with primary melanomas developed metastases, and 12 of them died. Among 105 patients with skin cancers 4 persons had recurrences and were irradiated repeatedly. The main merits of the method compared with surgery and X-ray therapy (the absence of side effects, promptness of the treatment, sterility and precise localization of the effect, vascular thrombosis contributing to an ablastic operation) are discussed. The results obtained with neodymium and CO2--lasers are compared.