Comparative proteome profiling of hydatid fluid from Algerian patients reveals cyst location-related variation in Echinococcus granulosus.

Human cystic echinococcosis, an endemic zoonosis in Algeria, is caused by larvae of the cestode Echinococcus granulosus. Parasitic modulation of the immune response allows E. granulosus to persist in intermediate hosts. Previous in vitro and in vivo immunological studies have shown differences in host immune responses according to the status and location of the hydatid cysts in the body. In this study, a proteomic analysis of human hydatid fluids was performed to identify the proteins in hydatid cyst fluids. Hydatid fluid was obtained after cystic surgical removal from three patients with these cysts. The study was conducted on fertile hydatid fluids from lungs, vertebra, and infertile paravertebral fluids. Comparisons of the protein compositions of these fluids revealed differences in their protein profiles. These differences are probably related to the cyst location and fertility status of the parasite. Notably, our analysis identified new proteins from the parasite and human host. The identification of host proteins in hydatid fluids indicates that the hydatid walls are permeable allowing a high protein exchange rate between the metacestode and the affected tissue. Interestingly, our study also revealed that parasite antigenic protein expression variations reflect the differences observed in host immunostimulation.

Protein 2DE reference map of the anterior midgut of the blood-sucking bug Rhodnius prolixus.

Rhodnius prolixus is an important vector of Trypanosoma cruzi, the causative agent of Chagas' disease, an illness that affects 20% of Latin America population. The obligatory course of the parasite in the vector digestive tract has made it an important target for investigation in order to control the parasite transmission and thus interrupt its biological cycle in the insect vector. Therefore, an insight into the vector midgut physiology is valuable for insect control as well as to provide potential novel targets for drugs and vaccines development and thus disease treatment. In this study, the first 2DE map of R. prolixus anterior midgut is described. Proteins were separated by 2DE and analyzed by nano-LC MS/MS. The results yielded 489 proteins from 475 spots. These proteins were classified into 28 functional groups and their physiological roles in the insect midgut are discussed. All MS data have been deposited in the ProteomeXchange with identifiers PXD001488 and PXD001489 (http://proteomecentral.proteomexchange.org/dataset/PXD001488, http://proteomecentral.proteomexchange.org/dataset/PXD001489).