RESUMO
We report synthesis and high-affinity hybridization of fully modified home-thymine 2'-deoxy and 2'-deoxy-2'-fluoro xylo nucleic acids.
Assuntos
Oligonucleotídeos/química , Oligonucleotídeos/síntese química , Xilose , Sequência de Bases , DNA Complementar/química , Hibridização de Ácido Nucleico , RNA Complementar/química , TiminaRESUMO
Xylo-Configured oligonucleotides (XNA) containing a novel conformationally restricted 2'-deoxy-2'-fluoro-beta-D-xylofuranosyl nucleotide monomer, a novel conformationally locked 2'-amino-2'-deoxy-2'-N,4'-C-methylene-beta-D-xylofuranosyl nucleotide monomer, and a known 2'-deoxy-beta-D-xylofuranosyl nucleotide monomer (XNA monomers) have been synthesized and their hybridization towards DNA and RNA complements studied. Thermal denaturation studies of nine-mer mixed-base sequences composed of a mixture of XNA monomers and DNA monomers revealed preferential hybridization towards RNA complements relative to DNA complements. For 14-mer homo-thymine XNAs containing thirteen XNA monomers, stable complexes towards single-stranded DNA and RNA were formed at pH 7. Gel-shift experiments revealed these complexes to involve at least two XNA strands per DNA or RNA target strand.