Regional tissue pO2, pCO2, pH and temperature measurement.

We compared the difference in brain tissue oxygen pressure (pO2), carbon dioxide pressure (pCO2), pH and temperature with 2 probes inserted 1 cm apart, in 7 patients. Following a craniotomy for cerebrovascular surgery, two Neurotrend probes which measure pO2, pCO2, pH and temperature were inserted into the brain 1 cm apart. Measures were compared between the probes under baseline anesthetic conditions and during the course of surgery. Under baseline conditions, tissue pO2, pCO2, pH and temperature were not different between the 2 probes. A significant correlation was seen between the probes in pH and temperature. During the course of surgery, variation in tissue gases and pH occurred with changes in ventilation and blood pressure but the difference between the probes remained stable. Ischemic changes in pO2, pCO2 and pH were seen in one of the 2 probes during brain artery occlusion or retractor placement. These results show that tissue pO2, pCO2 and pH are consistent in local brain regions during steady state conditions. The relationship between local measures is disrupted by regional ischemia.

Ultrasonographic and serologic abnormalities in Schistosoma japonicum infection in Leyte, the Philippines.

We have identified specific ultrasonographic changes in Schistosoma japonicum-infected patients associated with serologic indicators of general liver function. An ultrasonographic examination concomitant with hematologic and biochemical serum analyses was performed on 102 patients at the Schistosomiasis Hospital in Leyte, The Philippines. The ultrasonographic liver images were classified into four patterns, according to the development of periportal fibrosis and the patterns of echogenic bands. Eleven cases with a long-term infection showed typical septal formation (network pattern). Other ultrasonographic changes in the portal system, such as the severity of splenomegaly, did not correlate with the age of the study patients or the duration of their infection; however, the production of collateral vessels was clear in the group of older patients. Among various hematologic and biochemical serum indicators of liver damage, the serum levels of total bile acid (TBA) and procollagen-III-peptide (P-III-P) strongly correlated with the development of hepatic fibrosis and protal hypertension. These findings suggest that the ultrasonographic liver patterns classified here, along with the changes in serum levels of TBA and P-III-P, provide useful indicators for field monitoring of S. japonicum infection.

Improvement of ultrasonographic and serologic changes in Schistosoma japonicum-infected patients after treatment with praziquantel.

We previously reported ultrasonographic and serologic abnormalities in 102 patients infected with Schistosoma japonicum in Leyte, The Philippines. These patients were subsequently treated with praziquantel (3 x 20 mg/kg), and changes in ultrasonographic images and the serum levels of liver function markers in 52 patients were followed up every three months for a period of 17 months. Improvement in the thickening of the portal vein wall and the intensity of echogenic bands was detected six months after treatment with praziquantel. The level of splenomegaly was also reduced in 42 patients who originally did not show the production of collateral vessels. A significant decrease in the serum total bile acid (TBA) level was detected in all patients six months after treatment with praziquantel. However, significant ultrasonographic changes could not be detected in the patients classified as type 3, with severe hepatic fibrosis caused by the long-term infection. These results clearly show that ultrasonographic examination, along with data on the serum TBA level, provides a sensitive tool to monitor the severity of hepatic fibrosis and portal hypertension caused by S. japonicum infection, as well as the improvement resulting from praziquantel treatment.