Diversity and distribution of cultivable fungi present in acid sulphate soils in chronosequence under para-periglacial conditions in King George Island, Antarctica.

We recovered 85 fungal isolates from the acid sulphate soils in chronosequence under para-periglacial conditions in King George Island, Antarctica. Thirty-two taxa belonging to the phylum Ascomycota, Basidiomycota and Mortierellomycota were identified. Mortierella amoeboidea, Mortierella sp. 2, Mortierella sp. 3, Penicillium sp. 2 and Penicillium sp. 3 dominated the sulphite soils. Despite the multi-extreme physic-chemical conditions of the sulphate soils (low pH, variable content of macro and micronutrients and organic matter), the fungal assemblages exhibited moderate diversity indices, which ranged according to the degree of soil development. Soils with more weathered and, consequently, with highest values of organic carbon shelter the most diverse fungal assemblages, which can be associated with the occurrence of sulphurisation and sulphide oxidation. Different taxa of Mortierella and Penicillium displayed broad pH (3-9) and temperature (5-35 °C) plasticity. The multi-extreme sulphite soils of Antarctica revealed the presence of moderate fungal diversity comprising cold cosmopolitan and psychrophilic endemic taxa. Among these, Mortierella and Penicillium, known to survive in extreme conditions such as low temperature and available organic matter, low pH and high concentrations of metals, might represent interesting techniques to be used in biotechnological processes such as bioleaching in metallurgy and phosphate solubilisation in agriculture.

Fungi in glacial ice of Antarctica: diversity, distribution and bioprospecting of bioactive compounds.

We identified cultivable fungi present in the glacial ice fragments collected in nine sites across Antarctica Peninsula and assessed their abilities to produce bioactive compounds. Three ice fragments with approximately 20 kg were collected, melted and 3 L filtered through of 0.45 µm sterilized membranes, which were placed on the media Sabouraud agar and minimal medium incubated at 10 °C. We collected 66 isolates classified into 27 taxa of 14 genera. Penicillium palitans, Penicillium sp. 1, Thelebolus balaustiformis, Glaciozyma antarctica, Penicillium sp. 7, Rhodotorula mucilaginosa, and Rhodotorula dairenensis had the highest frequencies. The diversity and richness of the fungal community were high with moderate dominance. Penicillium species were present in all samples, with Penicillium chrysogenum showing the broadest distribution. P. chrysogenum, P. palitans, and Penicillium spp. had trypanocidal, leishmanicidal, and herbicidal activities, with P. chrysogenum having the broadest and highest capability. 1H NMR signals revealed the presence of highly functionalized secondary metabolites in the bioactive extracts. Despite extreme environmental conditions, glacial ice harbours a diverse fungal community, including species never before recorded in the Arctic and Antarctica. Among them, Penicillium taxa may represent wild fungal strains with genetic and biochemical pathways that may produce new secondary bioactive metabolites.