[Cycloferon biological activity characteristics].

AIM: Study the effect of cycloferon in experimental and clinical conditions on persistence properties of aurococci as well as features of their morpho-functional reaction by atomic force microscopy. MATERIALS AND METHODS: The study was carried out in 12 Staphylococcus aureus clones isolated from mucous membrane of nose anterior part of a resident carrier. The effect of cycloferon in vivo was evaluated in 26 resident staphylococci carriers under the control of anti-carnosine activity of staphylococci. Anti-carnosine activity was determined by O.V. Bukharin et al. (1999), biofilm formation -by G.A. O'Toole et al. (2000). Staphylococci treated with cycloferon were studied by atomic force microscopy in contact mode using scanning probe SMM-2000 microscope. RESULTS: The decrease of persistence properties of staphylococci under the effect of cycloferon in vitro and in vivo may be examined as one of the mechanisms of biological activity of the preparation. A significant increase of S. aureus surface roughness and changes in their morphology under the effect of cycloferon allow stating the disorder of barrier functions in the aurococci cell wall. CONCLUSION: The data obtained expand the understanding of cycloferon biological activity mechanisms.

[Diagnostic value of persistent characteristics of staphylococci during their carriage].

Comparative assessment of information value of significant persistent characteristics of staphylococci in state of carriage was made, and the usefulness of these characteristics for creating diagnostic models was defined. One hundred and fifty strains of Staphylococcus aureus and S. epidermidis isolated from healthy persons with subsequent differentiation of carriage types were used in the study. It has been established that strains isolated from resident carriers more often have more markedly expressed factors of persistence. Ranking the factors of persistence of staphylococci according to their information value during carriage allowed to identifythe leading sign among them--anti-carnosine activity. Using factor analysis, obtained signs were united in the single complex defining bacterial carriage, which allows to build a mathematical model permitting to diagnose staphylococcal carriage with 95% probability.