Report from the EPAA workshop: in vitro ADME in safety testing used by EPAA industry sectors.

There are now numerous in vitro and in silico ADME alternatives to in vivo assays but how do different industries incorporate them into their decision tree approaches for risk assessment, bearing in mind that the chemicals tested are intended for widely varying purposes? The extent of the use of animal tests is mainly driven by regulations or by the lack of a suitable in vitro model. Therefore, what considerations are needed for alternative models and how can they be improved so that they can be used as part of the risk assessment process? To address these issues, the European Partnership for Alternative Approaches to Animal Testing (EPAA) working group on prioritization, promotion and implementation of the 3Rs research held a workshop in November, 2008 in Duesseldorf, Germany. Participants included different industry sectors such as pharmaceuticals, cosmetics, industrial- and agro-chemicals. This report describes the outcome of the discussions and recommendations (a) to reduce the number of animals used for determining the ADME properties of chemicals and (b) for considerations and actions regarding in vitro and in silico assays. These included: standardisation and promotion of in vitro assays so that they may become accepted by regulators; increased availability of industry in vivo kinetic data for a central database to increase the power of in silico predictions; expansion of the applicability domains of in vitro and in silico tools (which are not necessarily more applicable or even exclusive to one particular sector) and continued collaborations between regulators, academia and industry. A recommended immediate course of action was to establish an expert panel of users, developers and regulators to define the testing scope of models for different chemical classes. It was agreed by all participants that improvement and harmonization of alternative approaches is needed for all sectors and this will most effectively be achieved by stakeholders from different sectors sharing data.

[Methicillin-resistant Staphylococcus aureus in medical and paramedical personnel returned from work in a foreign hospital]. / Meticillineresistente Staphylococcus aureus bij medisch en paramedisch personeel terug van werkbezoek aan een buitenlands ziekenhuis.

The cultures of two patients of the Surgical Intensive Care Unit (IC) of the Medical Centre of Utrecht University were found positive for methicillin-resistant Staphylococcus aureus (MRSA). A male nurse turned out to be the source, 4 months after his return from working in an English hospital. Cultures were, by mistake, not taken directly on arrival from abroad. Pulsed-field gel electrophoresis proved MRSA strains from both source and the 2 patients to be identical to a strain which was epidemic in Great Britain but had never occurred in the Netherlands. The IC has meanwhile been closed; at source investigation, 14 other patients and six staff members were found MRSA-positive. The policy in the hospital is to screen health care workers for MRSA carriership on return from an hospital abroad. The success of the policy depends strongly on the cooperation of health care workers in this matter.

["Mother" and "father" in psychoanalytic case vignettes. On some latent rules in the discourse of psychoanalysis]. / "Mutter" und "Vater" in psychoanalytischen Fallvignetten. Uber einige latente Regeln im Diskurs der Psychoanalyse.

Proceeding from the conviction that there is a collective gender-specific unconscious within the community of psychoanalysts which determines latent rules operative in psychoanalytic discourse, the authors have conducted a research project extending over a number of years and systematically examining case descriptions in psychoanalytic journals to see to what extent those latent gender-specific rules are in fact observed in such publications. The project examines the way in which male and female analysts employ the terms "mother" and "father" in these publications and also the frequency and connotations of concepts related to "mother" and "father". Significant differences between male and female analysts become apparent, substantiating the assumption of a collective gender-specific unconscious. The authors conclude that this unconscious finds its way into psychoanalytic therapy, leading to unconscious counter-transference reactions that are acted out rather than worked on.