Assuntos
Neoplasias da Mama/genética , Proteínas Proto-Oncogênicas/genética , DNA de Neoplasias/análise , Eletroforese em Gel de Poliacrilamida , Feminino , Amplificação de Genes , Regulação Neoplásica da Expressão Gênica , Humanos , Pessoa de Meia-Idade , Prognóstico , Estudos Prospectivos , Receptor ErbB-2RESUMO
Male Sprague-Dawley rats were exposed to N,N-dimethylacetamide (DMAC) and mated to untreated virgin females. Mean analytical exposure concentrations were 40, 116, and 386 ppm, respectively. A control group was exposed to air containing no DMAC. A total of 69 d of exposure to DMAC at these levels produced treatment-related effects of increased liver weights and liver/body weight ratios in the high- and medium-exposure groups of male rats. Reproductive data indicated no treatment-related effects on copulation efficiency or efficiency in effecting pregnancy, and there were no detectable treatment-related effects on preimplantation loss, postimplantation loss, embryotoxicity, or fetotoxicity in litters of females mated to males exposed to DMAC at the levels used in this study. The significance of these findings is discussed.
Assuntos
Acetamidas/toxicidade , Crioprotetores/toxicidade , Fertilidade/efeitos dos fármacos , Acetamidas/administração & dosagem , Administração por Inalação , Animais , Atrofia , Feminino , Rim/efeitos dos fármacos , Rim/patologia , Fígado/efeitos dos fármacos , Fígado/patologia , Masculino , Ratos , Ratos Endogâmicos , Valores de Referência , Testículo/efeitos dos fármacos , Testículo/patologiaRESUMO
A reversed-phase high performance liquid chromatographic (HPLC) procedure for the analyses of 3,4,4'-trichlorocarbanilide (TCC) and its free and/or conjugated metabolic products in plasma/serum in described. A rapid, effective clean-up procedure, prior to chromatographic evaluation, involves a single-step combined protein removal and THF extraction. Detection of the TCC moiety after HPLC separation is by UV absorption at 265 nm; quantitation by peak height measurement. A detection limit of 10 ppb of TCC and/or metabolities has been demonstrated for this method. Verification of this method was by radiotracer counting of the appropriate HPLC peaks from plasma of animals adminstered 14C-TCC/TCC. The utility of this method was demonstrated in both animal pharmacological and toxicological studies and in human bathing studies.