Minimizing pain in deer antler removal: Local anaesthetics in ZnO nanoparticle based collagen dressings as a promising solution.

Antler removal in deer is a common practice for various purposes, including meat production and traditional medicine. However, the current industry practice using lidocaine as a local anesthetic has limitations, such as short duration of action and the potential for postoperative infections. In this study, we investigated the performance of a ZnO collagen nanocomposites loaded with local anesthetics to improve wound management and alleviate pain associated with antler removal in red deer. The research involved the preparation of collagen nanocomposites with local anesthetics and testing the drug release rates using in vitro drug release tests. Pharmacokinetic analysis was performed to evaluate the total drug release from the collagen matrix in red deer after velvet removal. Additionally, the analgesic efficacy of these collagen nanocomposite dressings was assessed after antler removal in red deer. Functionalized ZnO nanoparticles were incorporated into collagen fibers to enhance their mechanical stability and prolong drug release. The developed collagen nanocomposites aimed to slowly release local anesthetics and promote wound healing. The findings of this research could have significant implications for improving the pain management and wound healing associated with antler removal in deer. The results obtained from the in vitro drug release tests, pharmacokinetic analysis, and analgesic efficacy evaluations provide valuable insights into the understanding and development of novel approaches for antler removal procedures in red deer. The findings contribute to the advancement of knowledge in this field and lay the foundation for future implementation of improved techniques and protocols for antler removal.

Harnessing the power of a photoinitiated thiol-ene "click" reaction for the efficient synthesis of S-lipidated collagen model peptide amphiphiles.

A photoinitiated thiol-ene "click" reaction was used to synthesize S-lipidated collagen model peptide amphiphiles. Use of 2-iminothiolane provided an epimerization-free thiol handle required for thiol-ene based incorporation of lipid moieties onto collagen-based peptide sequences. This approach not only led to improvements in the triple helical characteristics of the resulting collagen model peptides but also increased the aqueous solubility of the peptide amphiphiles. As a result, this methodology holds significant potential for the design and advancement of functional peptide amphiphiles, offering enhanced capabilities across a wide range of applications.

Towards the non-invasive assessment of staling in bovine hides with hyperspectral imaging.

Microbial spoilage or staling of bovine hides during storage leads to poor leather quality and increased chemical consumption during processing. Conventional microbiological examinations of hide samples which require time-consuming microbe culture cannot be employed as a practical staling detection approach for leather production. Hyperspectral imaging (HSI), featuring fast data acquisition and implementation flexibility has been considered ideal for in-line detection of microbial contamination in Agri- food products. In this study, a linescan hyperspectral imaging system working in a spectral range of 550 nm to 1700 nm was utilized as a rapid and non-destructive technique for predicting the aerobic plate counts (APC) on raw hide samples during storage. Fresh bovine hide samples were stored at 4 °C and 20 °C for 3 days. Every day, hyperspectral images were acquired on both sides for each sample. The APCs were determined simultaneously by conventional microbiological plating method. Leather quality was evaluated by microscopic inspection of grain surfaces, which indicate the acceptable threshold of microbe load on hide samples for leather processing. Partial least squares regression (PLSR) was applied to fit the spectral information extracted from the samples to the logarithmic values of APC to develop microbe load prediction models. All models showed good prediction accuracy, yielding a Rcv2 in the range of 0.74-0.92 and standard error of cross validation (SECV) in the range of 0.61-0.76 %. The prediction capability of the HSI was explored using the model developed with SNV + smoothened pre-processing to spatially predict plate count in the samples. Models established in this study successfully predicted the staling states characterised by bacterial loads on hide samples with low prediction errors. Models, visually, showed the differences in microbial load across the storage time and temperatures. Results illustrate that HSI can be potentially implemented as a non-invasive tool to predict microbe loads in bovine hides before leather processing, so that real-time grading of hides based on staling states can be achieved. This will reduce the cost of leather production and waste management and pave the way for allocating material supply for different production purposes.

Minimising chemical crosslinking for stabilising collagen in acellular bovine pericardium: Mechanistic insights via structural characterisations.

Chemically crosslinked acellular bovine pericardium (ABP) has been widely used in clinical practice as bioprostheses. To ensure its consistency and durability, crosslinkers are used in excess, with stability guided by indicators including the hydrothermal denaturation temperature, the enzymatic resistance and the degree of crosslinking. Yet, understanding of the intermolecular structure in collagen fibrils which imparts the intrinsic stability of the ABPs is lacking, and the discrepancies in the stability criteria in varied conditions are poorly explained. In this study, synchrotron small-angle X-ray scattering (SAXS) in combination with thermal and colorimetric methods are employed to investigate the changes in the structure and the stability of ABPs during crosslinking using glutaraldehyde (GA) or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) at different concentrations. Based on the findings, a mechanism is proposed to explicate the crosslinking effects on collagen structure and the relationship between the structure and each stability indicator. At low crosslinker concentrations, the telopeptidyl-helical linkages are preferred, which cause rearrangements in the intermolecular structure of collagen, and efficiently contribute to its stabilities. Excess crosslinking is revealed by a revert trend in structural changes and the plateauing of the stabilities, assigning to the helical-helical linkages and monovalent bindings. The former would improve thermal stability but not collagenase resistance, whereas the latter have negligible effects. Overall, this study provides a mechanistic understanding of the chemical crosslinking of ABPs, which will contribute to the future development of more efficient and economically viable strategies to produce bioprostheses. STATEMENT OF SIGNIFICANCE: Chemical crosslinking imparts suitable properties to acellular bovine pericardium (ABP) for clinical applications, yet the understanding is lacking on the structure-stability relationship especially under different crosslinking conditions. Structural evidence in this study differentiates the binding sites during crosslinking in collagen fibrils at different crosslinker concentrations, highlighting the excess usage in the conventional crosslinking treatments. The mechanism based on the structure of collagen also successfully explains the dissimilarity in hydrothermal and enzymatic stabilities with varied crosslinking conditions. Future researches focusing on developing biomaterials via chemical crosslinking of ABPs would benefit from this study, for its contribution to the better understanding of the relationship of collagen structure and functions.

Effect of Dialdehyde Carboxymethyl Cellulose Cross-Linking on the Porous Structure of the Collagen Matrix.

Porous structures are essential for some collagen-based biomaterials and can be regulated by crosslinkers. Herein, dialdehyde carboxymethyl cellulose (DCMC) crosslinkers with similar size but different aldehyde group contents were prepared through periodate oxidation of sodium carboxymethyl cellulose with varying degrees of substitution (DS). They can penetrate into the hierarchy of fibril and form inter-molecular and intra-fibril cross-linking within the collagen matrix due to their nanoscale sizes and reactive aldehyde groups. The collagen matrices possessed higher porosity, significantly greater proportion of large pores (Φ > 10 µm), and shorter D-periodicity after cross-linking, showing greater potential for biomedical applications. In addition, the crosslinked collagen matrices showed satisfactory biocompatibility and biodegradation. The decreased DS of carboxymethyl cellulose, which led to the increased aldehyde content of corresponding DCMC, brought about an enhanced cross-linking degree, porosity, and proportion of large pores of the crosslinked collagen matrix. DCMC dosage of 6% was sufficient for cross-linking and pore formation. Excess DCMC would physically deposit in the matrix and decrease the porosity instead. Therefore, the desired pore properties of the collagen matrix could be obtained by regulating the structure of DCMC and thereby achieving the required functions of the biomaterial.

Hierarchical Incorporation of Surface-Functionalized Laponite Clay Nanoplatelets with Type I Collagen Matrix.

Unraveling the interaction mechanisms of type I collagen with various inorganic nanoparticles is of pivotal importance to construct collagen-based bionanocomposites with hierarchical structures for biomedical, pharmaceutical, and other industrial applications. In this study, synthetic two-dimensional Laponite nanoplatelets (LAP NPs) are surface-functionalized with tetrakis(hydroxymethyl) phosphonium sulfate (THPS) for reinforcing their incorporation with type I collagen matrix by focusing on the influences of the interactions on the hierarchical structures of the collagen. Our results indicate that the LAP NPs can be successfully surface-functionalized with THPS via covalent bonds between the amine-functionalized NPs and the hydroxymethyl groups of THPS. Moreover, the resulting NPs can be well dispersed into the collagen matrix and evenly bound onto the collagen fiber strands and between the collagen fibrils, preserving the native D-periodic banding patterns of the collagen fibrils. The formation of covalent and hydrogen bonds between the collagen and the functionalized NPs can stabilize the intrinsic triple-helical conformation of the collagen, conferring the resulting collagen-based nanocomposites with improved thermal stability and enhanced mechanical properties. We anticipate that a fundamental understanding of the interactions between the collagen and functionalized inorganic nanoparticles would contribute to the design, fabrication, and further application of hierarchical collagen-based bionanocomposites with multifunctions.

Monitoring the Degradation of Collagen Hydrogels by Collagenase Clostridium histolyticum.

Collagen-based hydrogels are investigated extensively in tissue engineering for their tunable physiochemical properties, biocompatibility and biodegradability. However, the effect of the integrity of the collagen triple helical structure on biodegradability is yet to be studied. In this study, we monitored the degradation of intact collagen (C-coll) and hydrolyzed collagen (D-coll) hydrogels in collagenase Clostridium histolyticum to understand their degradation process. Our results show that when peptides are present on the surface of the fibrils of D-coll hydrogels, cleavage of amide bonds occur at a much higher rate. The fibrillar structure of D-coll hydrogel results in a more pronounced breakdown of the gel network and dissolution of collagen peptides. The results from this work will improve the understanding of enzymatic degradation and the resulting bioabsorption of collagen materials used in drug delivery systems and scaffolds.

Anion-regulated binding selectivity of Cr(III) in collagen.

We present a mechanism for the selectivity of covalent/electrostatic binding of the Cr(III) ion to collagen, mediated by the kosmotropicity of the anions. Although a change in the long-range ordered structure of collagen is observed after covalent binding (Cr(III)-OOC) in the presence of SO4 2- at pH 4.5, the νsym (COO- ) band remains intense, suggesting a relatively lower propensity for the Cr(III) to bind covalently instead of electrostatically through Cr(H2 O)6 3+ . Replacing SO4 2- with Cl- reduces the kosmotropic effect which further favors the electrostatic binding of Cr(III) to collagen. Our findings allow a greater understanding of mechanism-specific metal binding in the collagen molecule. We also report for the first time, surface-enhanced Raman spectroscopy to analyze binding mechanisms in collagen, suggesting a novel way to study chemical modifications in collagen-based biomaterials.

In situ structural studies during denaturation of natural and synthetically crosslinked collagen using synchrotron SAXS.

Collagen is an important biomacromolecule, making up the majority of the extracellular matrix in animal tissues. Naturally occurring crosslinks in collagen stabilize its intermolecular structure in vivo, whereas chemical treatments for introducing synthetic crosslinks are often carried out ex vivo to improve the physical properties or heat stability of the collagen fibres for applications in biomaterials or leather production. Effective protection of intrinsic natural crosslinks as well as allowing them to contribute to collagen stability together with synthetic crosslinks can reduce the need for chemical treatments. However, the contribution of these natural crosslinks to the heat stability of collagen fibres, especially in the presence of synthetic crosslinks, is as yet unknown. Using synchrotron small-angle X-ray scattering, the in situ role of natural and synthetic crosslinks on the stabilization of the intermolecular structure of collagen in skins was studied. The results showed that, although natural crosslinks affected the denaturation temperature of collagen, they were largely weakened when crosslinked using chromium sulfate. The development of synergistic crosslinking chemistries could help retain the intrinsic chemical and physical properties of collagen-based biological materials.

A guide to high-efficiency chromium (III)-collagen cross-linking: Synchrotron SAXS and DSC study.

The inefficiency of the chromium (III)-collagen cross-linking reaction during conventional leather processing results in severe environmental pollution from the waste chromium in the effluent. A mechanistic study using synchrotron-based small-angle X-ray scattering (SAXS) and differential scanning calorimetry (DSC) on ThruBlu tanned leather, revealed the effect of chromium sulphate and its pre-treatments on collagen structure and stability. By pre-treating with complexing agents such as sodium formate and disodium phthalate, as well as nanoclay (sodium montmorillonite), the uniformity through bovine hide collagen matrix were improved significantly. These pre-treatments effectively reduce the reactivity of chromium during its cross-linking reaction with collagen while retaining its bound water. However, collagen pre-treated with a covalent cross-linker (glutaraldehyde) results in a decrease in both chromium-collagen cross-linking and bound water while improving uniformity. These molecular-level insights can be developed into metrics to guide us towards a more sustainable future for the leather industry.

Solution Synthesis, Surface Passivation, Optical Properties, Biomedical Applications, and Cytotoxicity of Silicon and Germanium Nanocrystals.

Silicon and germanium nanocrystals (NCs) are attractive materials owing to their unique size and surface-dependent optical properties. The optical properties of silicon and germanium NCs make them highly suitable for a range of applications, including bioimaging, light-emitting diodes, and solar cells. In this review, the solution synthesis, surface passivation, optical properties, biomedical applications, and cytotoxicity of silicon and germanium NCs are compared and contrasted. Over the last 10 years, synthetic protocols have improved considerably, with size control readily achieved. Investigations have begun into a range of silicon and germanium nanostructures, including doped, alloy, and metal-semiconductor hybrid NCs, which represent the next generation of silicon and germanium nanomaterials. Silicon and germanium NCs are actively researched for a wide array of biomedical applications, including, long-term in vivo cellular imaging, fluorescent nanocarriers for drug delivery, and as contrast agents for magnetic resonance imaging (MRI). Cytotoxicity studies have shown the low toxicity of Si NCs, while demonstrating that Ge NCs are less toxic than CdSe NCs at similar concentrations, giving these materials a strong future in nanomedicine applications.

Nanoparticle cross-linked collagen shields for sustained delivery of pilocarpine hydrochloride.

Glaucoma is a common progressive eye disorder which remains the second leading cause of blindness worldwide. Current therapy involves frequent administration of eye drops which often results in poor patient adherence and therapeutic outcomes. The aim of this study was to overcome these limitations by developing a novel nanoparticle cross-linked collagen shield for sustained delivery of pilocarpine hydrochloride (PHCl). Three metal oxide nanoparticles (NPs); titanium dioxide (TiO2), zinc oxide (ZnO) and polyvinylpyrrolidone (PVP) capped zinc oxide (ZnO/PVP), were evaluated for their cytotoxicity as well as shield transparency before selecting ZnO/PVP NPs as the ideal candidate. Cross-linked collagen shields were then characterized for their mechanical strength, swelling capacity and bioadhesive properties, with ZnO/PVP NP cross-linked shields showing the most favorable characteristics compared to plain films. The shield with the best properties was then loaded with PHCl and in vitro release of zinc ions as well as PHCl was measured without and with further cross-linking by ultraviolet irradiation. The concentration of zinc ions released was well below the IC50 rendering them safe for ocular use. Moreover, collagen shields cross-linked with ZnO/PVP NPs released PHCl over a period of 14 days offering a promising sustained release treatment option for glaucoma.

New Visible-Light Photoinitiating System for Improved Print Fidelity in Gelatin-Based Bioinks.

Oxygen inhibition is a phenomenon that directly impacts the print fidelity of 3D biofabricated and photopolymerized hydrogel constructs. It typically results in the undesirable physical collapse of fabricated constructs due to impaired cross-linking, and is an issue that generally remains unreported in the literature. In this study, we describe a systematic approach to minimizing oxygen inhibition in photopolymerized gelatin-methacryloyl (Gel-MA)-based hydrogel constructs, by comparing a new visible-light initiating system, Vis + ruthenium (Ru)/sodium persulfate (SPS) to more conventionally adopted ultraviolet (UV) + Irgacure 2959 system. For both systems, increasing photoinitiator concentration and light irradiation intensity successfully reduced oxygen inhibition. However, the UV + I2959 system was detrimental to cells at both high I2959 concentrations and UV light irradiation intensities. The Vis + Ru/SPS system yielded better cell cyto-compatibility, where encapsulated cells remained >85% viable even at high Ru/SPS concentrations and visible-light irradiation intensities for up to 21 days, further highlighting the potential of this system to biofabricate cell-laden constructs with high shape fidelity, cell viability, and metabolic activity.

Silicon and germanium nanoparticles with tailored surface chemistry as novel inorganic fiber brightening agents.

Low-molecular-weight organic molecules, such as coumarins and stilbenes, are used commercially as fluorescent whitening agents (FWAs) to mask photoyellowing and to brighten colors in fabrics. FWAs achieve this by radiating extra blue light, thus changing the hue and also adding to the brightness. However, organic FWAs can rapidly photodegrade in the presence of ultraviolet (UV) radiation, exacerbating the yellowing process through a reaction involving singlet oxygen species. Inorganic nanoparticles, on the other hand, can provide a similar brightening effect with the added advantage of photostability. We report a targeted approach in designing new inorganic silicon- and germanium-based nanoparticles, functionalized with hydrophilic (amine) surface terminations as novel inorganic FWAs. When applied on wool, by incorporation in a sol-gel Si matrix, the inorganic FWAs improved brightness properties, demonstrated enhanced photostability toward UV radiation, especially the germanium nanoparticles, and also generated considerably lower levels of reactive oxygen species compared to a commercial stilbene-based organic FWA, Uvitex NFW.

Sized controlled synthesis, purification, and cell studies with silicon quantum dots.

This article describes the size control synthesis of silicon quantum dots with simple microemulsion techniques. The silicon nanocrystals are small enough to be in the strong confinement regime and photoluminesce in the blue region of the visible spectrum and the emission can be tuned by changing the nanocrystal size. The silicon quantum dots were capped with allylamine either a platinum catalyst or UV-radiation. An extensive purification protocol is reported and assessed using (1)H NMR to produce ultra pure silicon quantum dots suitable for biological studies. The highly pure quantum dots were used in cellular uptake experiments and monitored using confocal microscopy. The results showed that the amine terminated silicon nanocrystals accumulated in lysosome but not in nuclei and could be used as bio-markers to monitor cancer cells over long timescales.

Hot-injection synthesis of iron/iron oxide core/shell nanoparticles for T2 contrast enhancement in magnetic resonance imaging.

Here we report a new, bench-top synthesis for iron/iron oxide core/shell nanoparticles via the thermal decomposition of Fe(η(5)-C(6)H(3)Me(4))(2). The iron/iron oxide core/shell nanoparticles are superparamagnetic at room temperature and show improved negative contrast in T(2)-weighted MR imaging compared to pure iron oxides nanoparticles, and have a transverse relaxivity (r(2)) of 332 mM(-1) s(-1).

Colloidal synthesis of inorganic fullerene nanoparticles and hollow spheres of titanium disulfide.

The synthesis of inorganic fullerene (IF) nanoparticles and IF hollow spheres of titanium disulfide by a simple colloidal route is reported. The injection temperature of the titanium precursor into the solvent mixture was found to be important in controlling the morphology.

Chemical reactions on surface molecules attached to silicon quantum dots.

This Article describes research on chemical reactions on molecules attached to the surface of silicon quantum dots that have been performed to produce quantum dots with reactive surface functionalities such as diols and epoxides. Characterization of the surface reactions includes NMR and FT-IR studies, and the quantum dots were characterized by transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS). Cytotoxicity and cell viability assay conducted on silicon dots capped with polar molecules indicated low toxicity with quantum dots with more reactive functionalities found to be more toxic. The silicon quantum dots photoluminesce and have been used as a blue chromophore for the biological imaging of cells.