RESUMO
Solid-liquid nanocarriers (SLNs) are at the front of the rapidly emerging field of medicinal applications with a potential role in the delivery of bioactive agents. Here, we report a new SLN of natural deep eutectic solvent (NADES) and biotin-conjugated lysine-polyethylene glycol copolymer. The SLN system was analyzed for its functional groups, thermal stability, crystalline nature, particle size, and surface morphology through the instrumental analysis of FT-IR, TGA, XRD, DLS, SEM, and TEM. Encapsulation of PTX (paclitaxel) and 7-HC (7-hydroxycoumarin) with the SLN was carried out by dialysis, and UV-visible spectra evidenced the drug loading capacity and higher encapsulation efficiency obtained. The enhanced anticancer potential of PTX- and 7-HC-loaded SLN was assessed in vitro, and the system reduces the cell viability of MDA-MB-231 cells. The PTX- and 7-HC-loaded SLN system was investigated in a breast cancer-induced rat model via in vivo studies. It shows decreased lysosomal enzymes and increased levels of caspase to cure breast tumors. It very well may be reasoned that the designed PTX- and 7-HC-loaded SLN system has strong anticancer properties and exhibits potential for delivery of drug molecules in cancer treatment.
RESUMO
An amphiphilic polymer that consisted of a deep eutectic solvent (DES)-mediated drug carrier was designed, where the DES influenced the formation of folic acid (FA)-tagged g-ß-alanine-co-PCL polymer (DES@FA-g-ß-alanine-co-PCL); the nature of the carrier was investigated through emission analysis and pyrene used as a model probe (CMC = 0.4 mg/mL). The amphiphilic polymer was self-assembled into a sphere (≈204 nm diameter) with a surface charge of -3 ± 0.5 mV. The doxorubicin was incorporated and the structural changes were analyzed by UV-visible spectroscopy, FT-IR, XRD, Raman, and TGA analysis, while size and morphological analysis was performed by DLS, AFM, SEM, and TEM. The controlled release of drug from the carrier was observed at different pH levels. The enhanced anticancer potential of DOX-loaded polymeric micelle was studied both in vitro and in vivo breast cancer model. The treatment of DOX-loaded polymeric micelle reduces the viability and proliferation of MDA-MB-231 cells. From the results of the current investigation it concludes that the DOX-loaded polymeric micelle has enhance anticancer effect and it exhibits its potential effect at the dosage of 5 mg/kg body weight in mammary carcinoma-bearing rats. From the observed results, synthesized DOX-loaded polymeric micelle holds strong anticancer properties compared with free DOX and can be used as a potential carrier in the pharmaceutical industry.
RESUMO
Natural rubber Latex (Lax) is a colloidal dispersion of polymer particles in liquid and shows good biodegradable, biocompatibility, and non-toxicity. Natural polymers are the most important materials used in food packaging, micro/nano-drug delivery, tissue engineering, agriculture, and coating. In the present study, natural compounds extracted from plant Lax were designed to function as drug carriers using various surfactants via emulation and solvent evaporation method. Calotropis gigantea belongs to the family Apocynaceae and has received considerable attention in modern medicine, ayurvedeic, siddha, and traditional medicine. Since, we were isolated biodegradable, non-toxic, and biocompatible materials as latex from Calotropis gigantea plant. The Lax was separated as per their solubility nature and it was designed as a carrier using surfactant namely; Sorbitanmonolaurate (Span-20), sodium lauryl sulfate (SLS), and cetyltrimethylammonium bromide (CTAB). The isolated compounds from Lax of Calotropis gigantea were analyzed using high-performance liquid chromatography. To confirm the encapsulation efficiency and in vitro drug release of the carriers, doxorubicin (DOX) was used as a model natural drug. The hybrid nanocarriers were successfully synthesized through simple solvent evaporation using three surfactants, and the morphology was characterized by SEM and TEM technique. The functionality and crystalline nature of the nanocarriers were confirmed using FTIR and XRD, respectively. Within 90min, the maximum amount of DOX was encapsulated in the carriers, and prolonged cumulative drug release by the nanocarriers was observed. The formulated natural carriers were found to have potentially effective cytotoxic effects on lung cancer cells.
