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1.
Bioorg Med Chem Lett ; 28(2): 155-159, 2018 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-29198862

RESUMO

A set of triazole-based analogues of N-coumaroyltyramine was designed to discover potential leads that may help in the control of bacterial biofilms. the most potent compounds act as inhibitors of biofilm development with EC50 closed to ampicillin (EC50 = 11 µM) without toxic effect on bacterial growth even at high concentrations(100 µM).


Assuntos
Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Ácidos Cumáricos/farmacologia , Paracoccus/efeitos dos fármacos , Pseudoalteromonas/efeitos dos fármacos , Antibacterianos/síntese química , Antibacterianos/química , Ácidos Cumáricos/síntese química , Ácidos Cumáricos/química , Relação Dose-Resposta a Droga , Testes de Sensibilidade Microbiana , Estrutura Molecular , Paracoccus/crescimento & desenvolvimento , Pseudoalteromonas/crescimento & desenvolvimento , Relação Estrutura-Atividade
2.
Vet J ; 216: 38-44, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27687924

RESUMO

Bovine brucellosis is an infectious disease of worldwide public health and economic importance. The usual tests for the diagnosis of this disease include the Rose-Bengal test (RBT), complement fixation test (CFT), serum agglutination test (SAT) and indirect ELISA. New tests such as competitive ELISAs (C-ELISA) and fluorescence polarisation assay (FPA) have been developed. However, C-ELISA may correspond to different protocols and a wide variation may exist in their diagnostic performance. The aim of this study was to evaluate three commercially available C-ELISA kits (C-ELISA1-3) and FPA for the diagnosis of bovine brucellosis and compare test performance with RBT, CFT, indirect ELISA and FPA. Sera submitted to EU laboratories in 2011 from 5111 adult cattle were tested. Individual test sensitivities (Se) and specificities (Sp) were estimated. Threshold assessment using the receiver operating characteristic method was also performed. The most sensitive tests were FPA (99.0%; 95% confidence interval [CI], 97.9-100%), C-ELISA1 (98.4%; 95% CI, 97.0-99.8%) and RBT (97.7%; 95% CI, 95.9-99.3%). The most specific tests were CFT (99.98%; 95% CI, 99.93-100%), SAT (99.98%; 95% CI, 99.93-100%) and RBT (99.89%; 95% CI, 99.79-99.99%). Among the new tests, none of the three C-ELISA kits studied could be recommended as a single screening test because of their low specificity, especially when used in a herd. C-ELISA3 could not be recommended as confirmatory test on individual animals to determine whether false positive serological test results had occurred.


Assuntos
Testes de Aglutinação/veterinária , Brucella/isolamento & purificação , Brucelose Bovina/diagnóstico , Testes de Fixação de Complemento/veterinária , Ensaio de Imunoadsorção Enzimática/veterinária , Imunoensaio de Fluorescência por Polarização/veterinária , Rosa Bengala/química , Testes de Aglutinação/métodos , Animais , Brucelose Bovina/microbiologia , Bovinos , Testes de Fixação de Complemento/métodos , Ensaio de Imunoadsorção Enzimática/métodos , Imunoensaio de Fluorescência por Polarização/métodos , Sensibilidade e Especificidade
4.
Epidemiol Infect ; 143(1): 157-66, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24576504

RESUMO

In some French départements, the eradication of bovine tuberculosis is incomplete and usual skin tests [single intradermal tuberculin test (SIT) and single intradermal comparative cervical test (SICCT)] have poor specificity due to cross-reactions with non-pathogenic mycobacteria, causing economic losses. In Côte d'Or (Burgundy, France), an experimental serial testing scheme based on the combination of SICCT and gamma-interferon (IFN-γ) tests has been initiated in order to shorten the interval between suspicion and its invalidation in herds with false-positive results to skin tests. Our aim was to assess the scheme's sensitivity and to compare it to the sensitivity of the screening scheme recommended by the European Commission. Our study included 1768 animals from Côte d'Or. The sensitivities of both schemes were estimated using a Bayesian approach. The individual sensitivity of the IFN-γ test [88·1%, 95% credibility interval (CrI) 72·8-97·5] was not significantly different from individual SICCT sensitivity (80·3%, 95% CrI 61·6-98·0) and individual SIT sensitivity (84·2%, 95% CrI 59·0-98·2). The individual specificity of the IFN-γ test was 62·3% (95% CrI 60·2-64·5). No significant difference could be demonstrated between the sensitivities of the serial testing scheme used in Côte d'Or (73·1%, 95% CrI 41·1-100) and the European Union serial testing scheme (70·1%, 95% CrI 31·5-100·0).


Assuntos
Testes de Liberação de Interferon-gama/métodos , Programas de Rastreamento/métodos , Teste Tuberculínico/métodos , Tuberculose Bovina/diagnóstico , Animais , Bovinos , França , Sensibilidade e Especificidade
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