[Study of promutagen biotransformation in the Ames test. IV. The effect of transplanted tumors on the biotransformation of various antineoplastic agents]. / Issledovanie protsessov biotransformatsii promutagenov s pomoshch'iu testa Eimsa. Soobshchenie IV. Vliianie perevivaemykh opukholei na protsessy biotransformatsii nekotorykh protivoopukholevykh preparatov.

The effect of transplantation of rat tumours Jensen sarcoma, sarcoma 45, sarcoma M-1, as well as of inoculation of rat normal connective tissue on the processes of biotransformation of antitumour preparations cyclophosphane (CP), thiophosphamide, prospidine and of model compound nitrosomorpholine (NM) was studied. The study was accomplished by means of the Ames test with indicator bacterial strain Salmonella typhimurium TA 1950 in relation to the reactions of the 1st and the 2nd phases of xenobiotics metabolism. It was shown that the presence of tumours leads to inhibition of both metabolic activation processes of the promutagens NM and CP and the conjugation reactions of genetically active metabolites of these compounds with reduced glutathione. Genetic danger is supposed to be increased during application of antitumour preparations, the mutagenic activity of which is due to the activity of their metabolites. It is noted that the most essential effect on biotransformation processes of NM and CP was exhibited by sarcoma M-1, the most important changes of the biotransformation processes of promutagens being observed in the initial period of pathologic process, i.e. on the 3rd day after inoculation. Transplantation of the normal connective tissue of rats had no effect on reactions of both the 1st and the 2nd phase of metabolism of the promutagens studied.

[Experimental combined administration of cyclophosphane with serotonin, insulin and glucose]. / Kombinirovannoe primenenie tsiklofosfana s serotoninom, insulinom i gliukozoi v éksperimente

Experiments on mice with sarcoma 37 evidenced that the use of cyclophosphan in combination with serotonin, insulin, insulin + glucose and serotonin + glucose enable it to obtain a more significant antineoplasic effect than this is possible by the treatment with cyclophan alone. A combined application of these agents produced a more profound inhibition of the glycogen-forming function and restitution of the glycogen cintent in the liver of the test animals occurred quicker than when cyclophosphan alone was used. In oncological practice it is recommended to use combinations of cyclophosphan with serotonin, insulin, insulin + glucose and serotonin + glucose.

[Study of the pharmacokinetics of phosphemide in rats with the use of physico-chemical methods]. / Izuchenie farmakokinetiki fosfemida u krys s pomoshch'iu fiziko-khimicheskikh metodov

A study on the passage of phosphemide with urine and distribution of the drug over the organs and tissues of intact (without fumour) rats and those with sarcoma-45 showed the bulk of unchanged drug to be excreted together with the urine during the first 3-4 hours following its intravenous and intraperitoneal administration. In rats with sarcoma-45 phosphamide is passed with urine in a somewhat greater amount than in the intact animals. With intravenous application the rate of the drug elimination in intact rats and in the ones with carcoma-45 is virtually the same, being somewhat lower with intraperitoneal injection of phosphemide.