Assuntos
Doenças dos Genitais Masculinos/complicações , Hérnia Inguinal/complicações , Obstrução Intestinal/etiologia , Escroto , Idoso , Doenças dos Genitais Masculinos/diagnóstico por imagem , Hérnia Inguinal/diagnóstico por imagem , Humanos , Obstrução Intestinal/diagnóstico por imagem , Masculino , RadiografiaRESUMO
Spain's Salmonella surveillance system backed by regionally-based epidemiologists around the country made it possible to detect and halt the spread of a foodborne salmonellosis outbreak due to powdered infant formula contaminated with a lactose-fermenting strain of Salmonella virchow. Forty-eight cases involving children, mostly under 7 months old, were detected in 14 out of Spain's 17 Regions. The outbreak started in January and ended in June 1994. All cases were microbiologically confirmed. The implicated strain had a 3.6 kb plasmid, was susceptible to all antimicrobials tested except nitrofurantoin and was phagetype 4a. Isolates from 8 of 24 Brand "A" milk samples tested had the same characteristics as case isolates. All affected/suspect batches of Brand "A" milk were destroyed and the product withdrawn from sale, which led to the end of the outbreak. This incident underscores the importance of maintaining surveillance systems able to detect and prevent foodborne outbreaks and alert to the possibility of isolating unusual lactose-fermenting Salmonella serotypes in especially sensitive food products.
Assuntos
Surtos de Doenças , Microbiologia de Alimentos , Alimentos Infantis/efeitos adversos , Lactose/metabolismo , Leite/efeitos adversos , Intoxicação Alimentar por Salmonella/epidemiologia , Salmonella/isolamento & purificação , Animais , Estudos de Casos e Controles , Feminino , Humanos , Lactente , Alimentos Infantis/microbiologia , Recém-Nascido , Masculino , Testes de Sensibilidade Microbiana , Leite/microbiologia , Plasmídeos , Vigilância da População , Salmonella/genética , Salmonella/metabolismo , Intoxicação Alimentar por Salmonella/etiologia , Espanha/epidemiologia , Inquéritos e QuestionáriosRESUMO
The stability and catalytic efficacy of the L-2-haloacid dehalogenase isolated from Azotobacter sp. RC26 were studied after immobilisation on a DEAE Sephacel solid matrix. While the optimum temperature for the soluble dehalogenase falls in the range of 30-40 degrees C, the activity of the immobilised enzyme shows a four-fold increase at 60 degree C. Immobilisation on a plug-flow bioreactor extends the range of usable substrate concentration. The improved catalytic characteristics after immobilisation of the haloacid dehalogenase may be relevant for its possible utilization in biotechnological applications ranging from waste treatment to synthesis of stereoisomers.
Assuntos
Azotobacter/enzimologia , Enzimas Imobilizadas/metabolismo , Hidrolases/metabolismo , Catálise , DEAE-Celulose , Estabilidade Enzimática , Temperatura Alta , Concentração de Íons de Hidrogênio , Hidrolases/isolamento & purificação , Resinas de Troca Iônica , Cinética , TermodinâmicaAssuntos
Ginecomastia/induzido quimicamente , Nifedipino/efeitos adversos , Idoso , Humanos , MasculinoRESUMO
gamma-Aminobutyraldehyde dehydrogenase from Escherichia coli K-12 has been purified and characterized from cell mutants able to grow in putrescine as the sole carbon and nitrogen source. The enzyme has an Mr of 195,000 +/- 10,000 in its dimeric form with an Mr of 95,000 +/- 1,000 for each subunit, a pH optimum at 5.4 in sodium citrate buffer, and does not require bivalent cations for its activity. Km values are 31.3 +/- 6.8 microM and 53.8 +/- 7.4 microM for delta-1-pyrroline and NAD+, respectively. An inhibitory capacity for NADH is also shown using the purified enzyme.
