RESUMO
GDPmannose dehydrogenase (EC 1.1.1.132) in a mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis was identified by demonstrating the NAD-linked formation of GDPmannuronate from GDPmannose mediated by a cell extract of the organism. Nonmucoid mutant strains did not contain GDPmannose dehydrogenase, which suggests that the enzyme is involved in the biosynthesis of alginate-like polysaccharide by P. aeruginosa.
Assuntos
Alginatos/biossíntese , Desidrogenases de Carboidrato/metabolismo , Guanosina Difosfato Manose/metabolismo , Açúcares de Nucleosídeo Difosfato/metabolismo , Polissacarídeos Bacterianos/biossíntese , Pseudomonas aeruginosa/enzimologia , Pseudomonas aeruginosa/crescimento & desenvolvimentoAssuntos
Heparina/biossíntese , Fígado/enzimologia , Animais , Bovinos , Marcação por Isótopo , Ácidos Sulfúricos/análise , TrítioRESUMO
Fusion of beta-L-fucopyranose tetraacetate with phosphoric acid for 1 min at 50 degrees gives a 9:1 anomeric mixture of the alpha- and beta-pyranosyl phosphates. Longer fusion times give the alpha-anomer exclusively. The L-fucofuranose tetraacetates were synthesized for the first time by acetolysis of methyl-2,3,5-tri-O-acetyl-beta-L-fucofuranoside. Fusion of the furanose tetraacetates with phosphoric acid gave a mixture of the fucofuranosyl phosphates in which the beta-anomer predominated (beta/alpha= 2.4). Anomeric pairs in the fucofuranose series appear to be distinguishable by the chemical shift of the C-6 methyl protons, as already shown by Sinclair and Sleeter in the pyranose series.
