A cross-sectional analysis of zinc and copper levels and their relationship to cardiovascular disease risk markers in Qatar biobank participants.

Cardiovascular diseases (CVD) are the leading cause of mortality and morbidity worldwide. Dietary intake, particularly zinc (Zn) and copper (Cu) has been strongly associated with CVD. These trace elements play a crucial role in human enzyme activity, suppressing inflammation, catalyzing lipid metabolism enzymes, reducing oxidative stress, and regulating glucose metabolism. However, imbalances in these elements are linked to cardiovascular disturbances. Thus, this study aimed to investigate the association between circulating levels of Zn, Cu, and Zn/Cu ratio with CVD risk factors in the Qatari population. Bivariate logistic regression, adjusted for age, nationality, gender, and education was performed to examine the impact of Zn, Cu, and Zn/Cu ratio (as independent variables) on major CVD risk markers (as dependent variables). Participants in the highest Zn tertiles (T2 and T3) were at greater odds ratio (OR) of unfavorable metabolic functions such as elevated HbA1C [OR = 2.5, p = 0.015 (T2) and OR = 3.2, p = 0.002 (T3)], triglycerides [OR = 2.17, p = 0.015 (T2), and TyG index [OR = 2.21, p = 0.004 (T2), and OR = 2.67, p < 0.001 (T3)] compared to T1. Conversely, they had significantly lower ORs for prolonged prothrombin time [OR = 0.37, p = 0.001 (T3)]. Higher levels of Cu (T2 and T3) had higher OR for elevated HDL-C levels [OR = 1.69, p = 0.046 (T2), and OR = 2.27, p = 0.002 (T3)] and lower OR for elevated levels of triglycerides (OR = 0.4, p = 0.009, T3), diastolic blood pressure [OR = 0.41, p = 0.024 (T2), and OR = 0.47, p = 0.049 (T3)], and creatinine kinase (OR = 0.27, p = 0.014, T3) compared to T1. Higher levels of Cu (T2 and T3) were associated with a higher risk for elevated fibrinogen levels [OR = 3.1, p = 0.035 (T2), and OR = 5.04, p = 0.002 (T3)]. Additionally, higher Zn/Cu ratio (T2 and T3) were associated with lower ORs for elevated fibrinogen levels [OR = 0.3, p = 0.005 (T2), and OR = 0.27, p = 0.005 (T3)] compared to T1, indicating a lower risk of developing CVD. The study reveals a link between Zn, Cu, and the Zn/Cu ratio and cardiovascular disease risk. A higher Zn/Cu ratio may protect against CVD, while elevated Cu levels are linked to obesity, fibrinogen levels, and HbA1C. Maintaining optimal levels of these trace elements, either through diet or supplementation, may help reduce CVD risk.

Role of Inflammatory Cytokines, Growth Factors and Adipokines in Adipogenesis and Insulin Resistance.

Obesity, manifested by increased adiposity, represents a main cause of morbidity in the developed countries, causing increased risk of insulin resistance and type 2 diabetes mellitus. Recruitment of macrophages and activation of innate immunity represent the initial insult, which can be further exacerbated through secretion of chemokines and adipocytokines from activated macrophages and other cells within the adipose tissue. These events can impact adipogenesis, causing dysfunction of the adipose tissue and increased risk of insulin resistance. Various factors mediate adiposity and related insulin resistance including inflammatory and non-inflammatory factors such as pro and anti-inflammatory cytokines, adipokines and growth factors. In this review we will discuss the role of these factors in adipogenesis and development of insulin resistance and type 2 diabetes mellitus in the context of obesity. Understanding the molecular mechanisms that mediate adipogenesis and insulin resistance could help the development of novel therapeutic strategies for individuals at higher risk of insulin resistance and type 2 diabetes mellitus.

Regular, Intense Exercise Training as a Healthy Aging Lifestyle Strategy: Preventing DNA Damage, Telomere Shortening and Adverse DNA Methylation Changes Over a Lifetime.

Exercise training is one of the few therapeutic interventions that improves health span by delaying the onset of age-related diseases and preventing early death. The length of telomeres, the 5'-TTAGGG n -3' tandem repeats at the ends of mammalian chromosomes, is one of the main indicators of biological age. Telomeres undergo shortening with each cellular division. This subsequently leads to alterations in the expression of several genes that encode vital proteins with critical functions in many tissues throughout the body, and ultimately impacts cardiovascular, immune and muscle physiology. The sub-telomeric DNA is comprised of heavily methylated, heterochromatin. Methylation and histone acetylation are two of the most well-studied examples of the epigenetic modifications that occur on histone proteins. DNA methylation is the type of epigenetic modification that alters gene expression without modifying gene sequence. Although diet, genetic predisposition and a healthy lifestyle seem to alter DNA methylation and telomere length (TL), recent evidence suggests that training status or physical fitness are some of the major factors that control DNA structural modifications. In fact, TL is positively associated with cardiorespiratory fitness, physical activity level (sedentary, active, moderately trained, or elite) and training intensity, but is shorter in over-trained athletes. Similarly, somatic cells are vulnerable to exercise-induced epigenetic modification, including DNA methylation. Exercise-training load, however, depends on intensity and volume (duration and frequency). Training load-dependent responses in genomic profiles could underpin the discordant physiological and physical responses to exercise. In the current review, we will discuss the role of various forms of exercise training in the regulation of DNA damage, TL and DNA methylation status in humans, to provide an update on the influence exercise training has on biological aging.

Retraction Note: Assessing cardiorespiratory fitness of soccer players: is test specificity the issue?-a review.

The editors have retracted this article [1] because it contains substantial text and figure overlap with the following articles [2-5].

Dynamic stretching alone can impair slower velocity isokinetic performance of young male handball players for at least 24 hours.

There are many adult studies reporting static stretch (SS)-induced deficits and dynamic stretch (DS) performance improvements shortly after the intervention. However, there is only a single study examining stretch-induced performance changes with youth at 24 hours' post-stretch. The objective of this study was to examine physiological responses of young trained athletes at 24-hours after experiencing SS or DS protocols. Eight young male, elite handball players (age: 16.1±5.1 years) were tested prior to-, 3-minutes and 24-hours following the three conditions (DS, SS, Control) in a randomized and counterbalanced order. Similar volumes of SS (2 repetitions of 75s for each leg) and DS (5 repetitions of 30s for each leg) involved one stretch each for the quadriceps and hamstrings. Tests included (i) two 4s maximal voluntary isometric contractions (MVC) at 60° of knee flexion with 2-min rest, (ii) two maximal isokinetic contractions each at 60°/sec and 300°/sec with 1-min rest, and (iii) two drop jumps with 30-sec rest. To simulate a full warm-up, dynamic activity including 5 minutes of aerobic cycling (70 rpm; 1 kilopond), 4 submaximal isometric contractions and 4 drop jumps were instituted before the pre-tests and following the interventions. Two-way repeated measures ANOVAs revealed that 1) both the SS and control conditions exhibited knee extensor 60°.s-1 (SS:-10.3%; p = 0.04, Control: -8.7%; p = 0.07) and 300°.s-1 (SS: -12.9%; p = 0.005, Control: -16.3%; p = 0.02) isokinetic deficits at post-test, 2) DS impaired knee flexor 60°.s-1 isokinetic work and power-related measures at post-test (Work: -10.1%; p = 0.0006; Power: -19.1%; p = 0.08) and at 24-hours' post-test (Work: 9.9%; p = 0.023; Power: -9.6%; p = 0.01), 3) DS (12.07% and 10.47%) and SS (13.7% and 14.6%) enhanced knee flexor 300°.s-1 isokinetic force and power-related measures compared to control. In conclusion, testing-induced knee extensor isokinetic impairments were counterbalanced by DS, however the hip flexion DS could have produced minor muscle damage for at least 24-hours decreasing knee flexor forces and power at 60°.s-1.

Assessing Cardiorespiratory Fitness of Soccer Players: Is Test Specificity the Issue?-A Review.

It is important that players and coaches have access to objective information on soccer player's physical status for team selection and training purposes. Physiological tests can provide this information. Physiological testing in laboratories and field settings are very common, but both methods have been questioned because of their specificity and accuracy respectively. Currently, football players have their direct aerobic fitness assessed in laboratories using treadmills or cycle ergometers, whilst indirect measures (using estimation of aerobic performance) are performed in the field, typically comprising multiple shuttle runs back and forth over a set distance. The purpose of this review is to discuss the applied techniques and technologies used for evaluating soccer players' health and fitness variables with a specific focus on cardiorespiratory testing. A clear distinction of the functionality and the specificity between the field tests and laboratory tests is well established in the literature. The review findings prioritize field tests over laboratory tests, not only for commodity purpose but also for motivational and specificity reasons. Moreover, the research literature suggests a combination of various tests to provide a comprehensive assessment of the players. Finally, more research needs to be conducted to develop a specific and comprehensive test model through the combination of various exercise modes for soccer players.