RESUMO
Low-voltage-activated T-type calcium channels are essential contributors to the functioning of thalamocortical neurons by supporting burst-firing mode of action potentials. Enhanced T-type calcium conductance has been reported in the Genetic Absence Epilepsy Rat from Strasbourg (GAERS) and proposed to be causally related to the overall development of absence seizure activity. Here, we show that calnexin, an endoplasmic reticulum integral membrane protein, interacts with the III-IV linker region of the Cav3.2 channel to modulate the sorting of the channel to the cell surface. We demonstrate that the GAERS missense mutation located in the Cav3.2 III-IV linker alters the Cav3.2/calnexin interaction, resulting in an increased surface expression of the channel and a concomitant elevation in calcium influx. Our study reveals a novel mechanism that controls the expression of T-type channels, and provides a molecular explanation for the enhancement of T-type calcium conductance in GAERS.
Assuntos
Canais de Cálcio Tipo T/genética , Canais de Cálcio Tipo T/metabolismo , Calnexina/metabolismo , Epilepsia Tipo Ausência/genética , Mutação de Sentido Incorreto , Animais , Modelos Animais de Doenças , Proteínas Mutantes/genética , Proteínas Mutantes/metabolismo , Transporte Proteico , RatosRESUMO
Low-voltage-activated T-type calcium channels are essential contributors to neuronal physiology where they play complex yet fundamentally important roles in shaping intrinsic excitability of nerve cells and neurotransmission. Aberrant neuronal excitability caused by alteration of T-type channel expression has been linked to a number of neuronal disorders including epilepsy, sleep disturbance, autism, and painful chronic neuropathy. Hence, there is increased interest in identifying the cellular mechanisms and actors that underlie the trafficking of T-type channels in normal and pathological conditions. In the present study, we assessed the ability of Stac adaptor proteins to associate with and modulate surface expression of T-type channels. We report the existence of a Cav3.2/Stac1 molecular complex that relies on the binding of Stac1 to the amino-terminal region of the channel. This interaction potently modulates expression of the channel protein at the cell surface resulting in an increased T-type conductance. Altogether, our data establish Stac1 as an important modulator of T-type channel expression and provide new insights into the molecular mechanisms underlying the trafficking of T-type channels to the plasma membrane.
Assuntos
Canais de Cálcio Tipo T/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Canais de Cálcio Tipo T/fisiologia , Membrana Celular/metabolismo , Células HEK293 , Humanos , Proteínas do Tecido Nervoso/fisiologiaRESUMO
Myelinated axons efficiently transmit information over long distances. The apposed myelin sheath confers favorable electrical properties, but restricts access of the axon to its extracellular milieu. Therefore, axonal metabolic support may require specific axo-myelinic communication. Here we explored activity-dependent glutamate-mediated signaling from axon to myelin. 2-Photon microscopy was used to image Ca(2+) changes in myelin in response to electrical stimulation of optic nerve axons ex vivo. We show that optic nerve myelin responds to axonal action potentials by a rise in Ca(2+) levels mediated by GluN2D and GluN3A-containing NMDA receptors. Glutamate is released from axons in a vesicular manner that is tetanus toxin-sensitive. The Ca(2+) source for vesicular fusion is provided by ryanodine receptors on axonal Ca(2+) stores, controlled by L-type Ca(2+) channels that sense depolarization of the internodal axolemma. Genetic ablation of GluN2D and GluN3A subunits results in greater lability of the compact myelin. Our results support the existence of a novel synapse between the axon and its myelin, suggesting a means by which traversing action potentials can signal the overlying myelin sheath. This may be an important physiological mechanism by which an axon can signal companion glia for metabolic support or adjust properties of its myelin in a dynamic manner. The axo-myelinic synapse may contribute to learning, while its disturbances may play a role in the pathophysiology of central nervous system disorders such as schizophrenia, where subtle abnormalities of myelinated white matter tracts have been shown in the human, or to frank demyelinating disorders such as multiple sclerosis.
Assuntos
Axônios/fisiologia , Bainha de Mielina/fisiologia , Fibras Nervosas Mielinizadas/fisiologia , Nervo Óptico/fisiologia , Sinapses/fisiologia , Animais , Axônios/ultraestrutura , Sinalização do Cálcio/fisiologia , Masculino , Camundongos , Camundongos Knockout , Bainha de Mielina/ultraestrutura , Fibras Nervosas Mielinizadas/ultraestrutura , Nervo Óptico/ultraestrutura , Ratos , Ratos Long-Evans , Receptores de N-Metil-D-Aspartato/fisiologia , Sinapses/ultraestruturaRESUMO
Commentary to: Functional changes in pyramidal neurons in the chronic methamphetamine-treated rat. (Gen. Physiol. Biophys. 2015, pp.5-12).
Assuntos
Metanfetamina/administração & dosagem , Neurônios/patologia , Córtex Piriforme/patologia , Células Piramidais/patologia , Animais , MasculinoRESUMO
Neuronal voltage-gated calcium channels have evolved as one of the most important players for calcium entry into presynaptic endings responsible for the release of neurotransmitters. In turn, and to fine-tune synaptic activity and neuronal communication, numerous neurotransmitters exert a potent negative feedback over the calcium signal provided by G protein-coupled receptors. This regulation pathway of physiologic importance is also extensively exploited for therapeutic purposes, for instance in the treatment of neuropathic pain by morphine and other µ-opioid receptor agonists. However, despite more than three decades of intensive research, important questions remain unsolved regarding the molecular and cellular mechanisms of direct G protein inhibition of voltage-gated calcium channels. In this study, we revisit this particular regulation and explore new considerations.
Assuntos
Canais de Cálcio/metabolismo , Proteínas de Ligação ao GTP/metabolismo , Neurônios/metabolismo , Animais , Bloqueadores dos Canais de Cálcio/farmacologia , Canais de Cálcio/química , Subunidades beta da Proteína de Ligação ao GTP/metabolismo , Subunidades gama da Proteína de Ligação ao GTP/metabolismo , Humanos , Terminações Pré-Sinápticas/metabolismo , Conformação Proteica , Receptores Acoplados a Proteínas G/metabolismoRESUMO
Neuronal abnormalities in neurodegenerative disorders such as Huntington disease, Alzheimer disease or Parkinson disease have been the primary focus of decades of research. However, increasing evidences indicate that glial cells and more specifically astrocytes could be as important players as their big brother. It is now particularly evident in Huntington disease where astrocytal potassium channels have emerged as a likely key factor in the pathogenesis of the disease.
RESUMO
Perhaps one of the most remarkable features of T-type calcium channels is their low-threshold of activation that makes these channels important candidates for calcium entry near the resting membrane potential of neurons. Hence, they mediate low-threshold burst discharges that occur during different forms of neuronal rhythmogenesis, but play also important roles in sensory transmission, as well as hormone and neurotransmitter release. Additionally, they have been implicated in an increasing number of neuronal pathologies including neuropathy, autism spectrum disorders and some forms of epilepsy. More recently, an implication of T-type calcium channel in the processing of Amyloid Precursor Protein was documented, with possible implication in the pathogenesis of Alzheimer's disease.
RESUMO
Neurodegenerative diseases are a burden of our century. Although significant efforts were made to find a cure or relief to this scourge, their pathophysiology remains vague and the cellular function of the key involved proteins is still unclear. However, in the case of amyloid ß (Aß), a key protein concerned in Alzheimer disease, we are now a step closer in the unscrambling of its cellular functions. Interestingly, whereas the exact role of Aß in the pathophysiology of Alzheimer disease is still unresolved, a recent study revealed a neuroprotective function of Aß in multiple sclerosis with possibly promising therapeutic benefits.
RESUMO
To date, nearly 35.6 million people world wide live with dementia, and the situation is going to get worse by 2050 with 115.4 million cases.(1) In the western world, the prevalence for dementia in people over the age of 60 is greater than 5% and two thirds are due to Alzheimer disease,(2) (-) (5) the most common form of dementias. Alzheimer disease (AD), first described as "presenile dementia" by the German psychiatrist and neuropathologist Alois Alzheimer in 1906,(6) is a devastating disease characterized by progressive cognitive deterioration, as well as impairments in behavior, language, and visuospatial skills.(7) Furthermore, Alzheimer discovered the presence of intraneuronal tangles and extracellular amyloid plaques in the diseased-damaged brain, the hallmarks of Alzheimer disease.
RESUMO
The use of ion channels to control defined events in defined cell types at defined times in the context of living tissue or whole organism represent one of the major advance of the last decade, and optogenetics (i.e the combination of genetic and optical methods) obviously played a key role in this achievement.(1) Although the existence of light-activated ion channels (i.e ospin channels) has been known since 1971,(2) it took about 35 y before the concept of an ion channel used for bioengineering control of cell or tissue activity becomes reality.(3) From that moment forward, rhodopsine channels(4) (,) (5) (i.e blue light-gated non-specific Na(+) channels that depolarize cells thus increasing cell excitability) or halorhodopsin channels(6) (i.e yellow light-gated Cl(-) channels that hyperpolarize cells thus decreasing cell excitability) have been extensively used to turn neurons on and off in response to diverse colors of light, with an extremely high temporal precision (i.e milliseconds range). Although optogenetics has been originally established in neuroscience, it addresses now to non-neuronal systems, including cardiac, smooth and skeletal muscles, glial cells or even embryonic stem cells.(7) (-) (9) However, although light stimulation allows control of cell excitability with a high spatio-temporal specificity, light waves present the disadvantage to not penetrate deep tissue, and implanted devices are required for in vivo light stimulation. In contrast to visible light-waves, radio-waves (i.e longer wavelength and lower frequency) can penetrate deep tissues with minimal energy absorption.
RESUMO
Heat shock proteins (Hsps) are a set of molecular chaperones involved in cellular repair. They provide protective mechanisms that allow cells to survive potentially lethal insults, In response to a conditioning stress their expression is increased. Here we examined the connection between Hsps and Aß(42), the amyloid peptide involved in the pathological sequence of Alzheimer's disease (AD). Extracellular Aß(42) associates with neuronal cells and is a major constituent of senile plaques, one of the hallmarks of AD. Although Hsps are generally thought to prevent accumulation of misfolded proteins, there is a lack of mechanistic evidence that heat shock chaperones directly modulate Aß(42) toxicity. In this study we show that neither extracellular Aß(42) nor Aß(42/)PrP(C) trigger the heat shock response in neurons. To address the influence of the neuroprotective heat shock response on cellular Aß(42), Western analysis of Aß(42) was performed following external Aß(42) application. Five hours after a conditioning heat shock, Aß(42) association with CAD cells was increased compared to control neurons. However, at forty-eight hours following heat shock Aß(42) levels were reduced compared to that found for control cells. Moreover, transient transfection of the stress induced Hsp40, decreased CAD levels of Aß(42). In contrast to CAD cells, hippocampal neurons transfected with Hsp40 retained Aß(42) indicating that Hsp40 modulation of Aß(42) proteostasis is cell specific. Mutation of the conserved HPD motif within Hsp40 significantly reduced the Hsp40-mediated Aß(42) increase in hippocampal cultures indicating the importance of this motif in regulating cellular Aß(42). Our data reveal a biochemical link between Hsp40 expression and Aß(42) proteostasis that is cell specific. Therefore, increasing Hsp40 therapeutically with the intention of interfering with the pathogenic cascade leading to neurodegeneration in AD should be pursued with caution.
Assuntos
Peptídeos beta-Amiloides/metabolismo , Espaço Extracelular/metabolismo , Proteínas de Choque Térmico HSP40/metabolismo , Fragmentos de Peptídeos/metabolismo , Doença de Alzheimer/metabolismo , Doença de Alzheimer/patologia , Animais , Bovinos , Linhagem Celular Tumoral , Demência/metabolismo , Demência/patologia , Regulação da Expressão Gênica , Hipocampo/citologia , Humanos , Cinética , Camundongos , Príons/metabolismo , RatosRESUMO
T-type calcium channels represent a key pathway for Ca(2+) entry near the resting membrane potential. Increasing evidence supports a unique role of these channels in fast and low-threshold exocytosis in an action potential-independent manner, but the underlying molecular mechanisms have remained unknown. Here, we report the existence of a syntaxin-1A/Ca(v)3.2 T-type calcium channel signaling complex that relies on molecular determinants that are distinct from the synaptic protein interaction site (synprint) found in synaptic high voltage-activated calcium channels. This interaction potently modulated Ca(v)3.2 channel activity, by reducing channel availability. Other members of the T-type calcium channel family were also regulated by syntaxin-1A, but to a smaller extent. Overexpression of Ca(v)3.2 channels in MPC 9/3L-AH chromaffin cells induced low-threshold secretion that could be prevented by uncoupling the channels from syntaxin-1A. Altogether, our findings provide compelling evidence for the existence of a syntaxin-1A/T-type Ca(2+) channel signaling complex and provide new insights into the molecular mechanism by which these channels control low-threshold exocytosis.
Assuntos
Canais de Cálcio Tipo T/metabolismo , Exocitose/fisiologia , Complexos Multiproteicos/metabolismo , Transdução de Sinais/fisiologia , Sintaxina 1/metabolismo , Canais de Cálcio Tipo T/genética , Linhagem Celular , Humanos , Complexos Multiproteicos/genética , Sintaxina 1/genéticaRESUMO
Parkinson's disease (PD) is a common neurodegenerative disease characterized by the loss of dopaminergic neurons in the substantia nigra and the aggregation of α-synuclein into Lewy bodies. Existing therapies address motor dysfunction but do not halt progression of the disease. A still unresolved question is the biochemical pathway that modulates the outcome of protein misfolding and aggregation processes in PD. The molecular chaperone network plays an important defensive role against cellular protein misfolding and has been identified as protective in experimental models of protein misfolding diseases like PD. Molecular mechanisms underlying chaperone-neuroprotection are actively under investigation. Current evidence implicates a number of molecular chaperones in PD including Hsp25, Hsp70 and Hsp90, however their precise involvement in the neurodegenerative cascade is unresolved. The J protein family (DnaJ or Hsp40 protein family) has long been known to be important in protein conformational processes.We assessed sensory and motor function of control and PD rats and then evaluated the brain region-specific expression levels of select J proteins by Western analysis. Surprisingly, we observed a widespread 26 kDa breakdown product of the J protein, TID1, (tumorous imaginal discs, mtHsp40 or DnaJ3) in a 6-hydroxydopamine (6-OHDA) rat model of PD in which food handling, gait symmetry and sensory performance were impaired. Greater behavioral deficits were associated with lower TID1 expression. Furthermore, direct application of either 6-OHDA or MPP+ (1-methyl-4-phenylpyridinum) to CAD (CNS-derived catecholinaminergic neuronal cell line) cell cultures, reduced TID1 expression levels.Our results suggest that changes in cellular TID1 are a factor in the pathogenesis of PD by impeding functional and structural compensation and exaggerating neurodegenerative processes. In contrast, no changes were observed in CSPα, Hsp40, Hsp70, Hsc70 and PrP(C) levels and no activation of caspase3 was observed. This study links TID1 to PD and provides a new target for therapeutics that halts the PD progression.
Assuntos
Regulação da Expressão Gênica/efeitos dos fármacos , Proteínas de Choque Térmico HSP40/metabolismo , Oxidopamina/farmacologia , Doença de Parkinson/etiologia , Doença de Parkinson/metabolismo , 1-Metil-4-fenilpiridínio/farmacologia , Animais , Linhagem Celular Tumoral , Modelos Animais de Doenças , Feminino , Proteínas de Choque Térmico HSP40/química , Homeostase/efeitos dos fármacos , Camundongos , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Peso Molecular , Doença de Parkinson/patologia , Doença de Parkinson/fisiopatologia , Desempenho Psicomotor/efeitos dos fármacos , Ratos , Ratos Long-Evans , Transdução de Sinais/efeitos dos fármacos , alfa-Sinucleína/metabolismoRESUMO
BACKGROUND: Cysteine string protein (CSPalpha) is a synaptic vesicle protein that displays unique anti-neurodegenerative properties. CSPalpha is a member of the conserved J protein family, also called the Hsp40 (heat shock protein of 40 kDa) protein family, whose importance in protein folding has been recognized for many years. Deletion of the CSPalpha in mice results in knockout mice that are normal for the first 2-3 weeks of life followed by an unexplained presynaptic neurodegeneration and premature death. How CSPalpha prevents neurodegeneration is currently not known. As a neuroprotective synaptic vesicle protein, CSPalpha represents a promising therapeutic target for the prevention of neurodegenerative disorders. METHODOLOGY/PRINCIPAL FINDINGS: Here, we demonstrate that the flavonoid quercetin promotes formation of stable CSPalpha-CSPalpha dimers and that quercetin-induced dimerization is dependent on the unique cysteine string region. Furthermore, in primary cultures of Lymnaea neurons, quercetin induction of CSPalpha dimers correlates with an inhibition of synapse formation and synaptic transmission suggesting that quercetin interfers with CSPalpha function. Quercetin's action on CSPalpha is concentration dependent and does not promote dimerization of other synaptic proteins or other J protein family members and reduces the assembly of CSPalpha:Hsc70 units (70kDa heat shock cognate protein). CONCLUSIONS/SIGNIFICANCE: Quercetin is a plant derived flavonoid and popular nutritional supplement proposed to prevent memory loss and altitude sickness among other ailments, although its precise mechanism(s) of action has been unclear. In view of the therapeutic promise of upregulation of CSPalpha and the undesired consequences of CSPalpha dysfunction, our data establish an essential proof of principle that pharmaceutical agents can selectively target the neuroprotective J protein CSPalpha.
Assuntos
Proteínas de Choque Térmico HSP40/efeitos dos fármacos , Proteínas de Membrana/efeitos dos fármacos , Quercetina/farmacologia , Transmissão Sináptica/efeitos dos fármacos , Animais , Dimerização , Proteínas de Choque Térmico HSP40/genética , Proteínas de Choque Térmico HSP40/fisiologia , Proteínas de Membrana/genética , Proteínas de Membrana/fisiologia , Camundongos , Camundongos KnockoutRESUMO
Until now it is still not clear which structural elements of the prion protein (PrP) are involved in its conversion process. Characterisation of these essential regions would help to understand the conversion process itself and might help to develop specific therapeutic approaches to inhibit PrP(res) formation by dominant inhibitory mutations. To address this important question 33 evenly spaced insertion mutants were generated spanning the entire sequence of the murine 3F4-tagged PrP. The mutants were expressed by retroviral transduction in three different scrapie infected cell lines (ScN2a; SMB[RC040]; SMB[22F]). The convertibility was affected not only by introducing the insertion in the putatively refolded region (aa100-170), but also in the C-terminus of PrP (up to aa214). Moreover, dominant inhibitory effects on conversion were observed for PrP-mutants at four distinguished regions (aa100-112; aa130-154; aa166-172, aa196-200). Computer based structural analysis revealed that these segments were organized in two structurally clearly separated regions supporting the idea that they could function as protein-protein interaction sites which are necessary during seed formation.
Assuntos
Regulação da Expressão Gênica , Mutagênese Insercional , Proteínas PrPSc/metabolismo , Sequência de Aminoácidos , Animais , Linhagem Celular , Glicosilação , Camundongos , Modelos Moleculares , Dados de Sequência Molecular , Proteínas PrPSc/química , Proteínas PrPSc/genética , Domínios e Motivos de Interação entre Proteínas , Isoformas de Proteínas , Estrutura Terciária de Proteína , Transporte ProteicoRESUMO
In response to a conditioning stress, the expression of a set of molecular chaperones called heat shock proteins is increased. In neurons, stress-induced and constitutively expressed molecular chaperones protect against damage induced by ischemia and neurodegenerative diseases, however the molecular basis of this protection is not known. Here we have investigated the crosstalk between stress-induced chaperones and cysteine string protein (CSPalpha). CSPalpha is a constitutively expressed synaptic vesicle protein bearing a J domain and a cysteine rich "string" region that has been implicated in the long term functional integrity of synaptic transmission and the defense against neurodegeneration. We have shown previously that the CSPalpha chaperone complex increases isoproterenol-mediated signaling by stimulating GDP/GTP exchange of Galpha(s). In this report we demonstrate that in response to heat shock or treatment with the Hsp90 inhibitor geldanamycin, the J protein Hsp40 becomes a major component of the CSPalpha complex. Association of Hsp40 with CSPalpha decreases CSPalpha-CSPalpha dimerization and enhances the CSPalpha-induced increase in steady state GTP hydrolysis of Galpha(s). This newly identified CSPalpha-Hsp40 association reveals a previously undescribed coupling of J proteins. In view of the crucial importance of stress-induced chaperones in the protection against cell death, our data attribute a role for Hsp40 crosstalk with CSPalpha in neuroprotection.
Assuntos
Proteínas de Choque Térmico HSP40/metabolismo , Resposta ao Choque Térmico , Proteínas de Membrana/metabolismo , Animais , Benzoquinonas/farmacologia , Linhagem Celular Tumoral , Lactamas Macrocíclicas/farmacologia , Camundongos , Chaperonas Moleculares , Complexos Multiproteicos , Neuroblastoma/patologia , Ligação Proteica , Multimerização Proteica , Receptor Cross-TalkRESUMO
Resistance to the antibiotic tetracycline (Tc) is regulated by its binding as a Tc:Mg2+ complex to the Tet Repressor protein (TetR). Tc:TetR recognition is a complex problem, with the protein and ligand each having several possible conformations and protonation states, which are difficult to elucidate by experiment alone. We used a combination of free-energy simulations and crystallographic analysis to investigate the electrostatic interactions between protein and ligand and the possible role of induced fit in Tc binding. Tc in solution was described quantum mechanically, while Tc:TetR interactions were described by a recent, high-quality molecular-mechanics model. The orientations of the amide and imidazole groups were determined experimentally by a careful analysis of Debye-Waller factors in alternate crystallographic models. The agreement with experiment for these orientations suggested that the simulations and their more detailed, thermodynamic predictions were reliable. We found that the ligand prefers an extended, zwitterionic state both in solution and in complexation with the protein. Tc is thus preorganized for binding, while the protein combines lock-and-key behavior for regions close to the ligand's amide, enolate, and ammonium groups, with an induced fit for regions close to the Mg2+ ion. These insights and the modeling techniques employed should be of interest for engineering improved TetR ligands and improved TetR proteins for gene regulation, as well as for drug design.