[The protective properties of the outer membranes of Francisella tularensis in an experimental infection in guinea pigs]. / Zashchitnye svoistva vneshnykh membran Francisella tularensis pri éksperimental'noi infektsii morskikh svinok.

Subcutaneous immunization, made in a single injection, with outer membrane preparations obtained from F.tularensis vaccine strain 15 and virulent strain A'Cole results in intensive immunity to tularemia in guinea pigs, ensuring the protection of 60-100% of the animals within a month after challenge with F.tularensis virulent strain 503 in a dose of 1,000 DCL. The development of protective effect induced by F.tularensis outer membranes can be observed during the first 24 hours and reaches its maximum by days 15-21 after immunization.

[Biochemical, antigenic and protective properties of the outer membrane of tularemia pathogens]. / Izuchenie biokhimicheskikh, antigennykh i protektivnykh svoistv vneshnei membrany vozbuditelia tuliaremii.

The outer membranes of Francisella tularensis were studied. The membranes were identified morphologically, immunologically and biochemically. They contained 12-20% of protein, 15-30% of carbohydrates, up to 40% of lipids. The main integral proteins of the outer membranes were the 47, 43, 17 and 12 kD proteins. The main protein 63 kD was not integral. The lipopolysaccharides isolated from the outer membranes and acetone-dried cells did not possess the protective properties in experimental tularemia. The preparations of outer membranes possessed the protective properties for mice infected with the virulent strain 503. Chitosan amplified the protective properties of outer membranes.