RESUMO
Fractionated phospholipids (PL) from soya beans were tried as partial thromboplastin (PT) in the activated partial thromboplastin time test. PL fractions were investigated for content of individual phospholipid classes, acidity and procoagulant activity as were PT from human brain and chromatographically pure PL. Procoagulant activity of phospholipid mixtures is higher than that of chromatographically pure PL and is unrelated to PL variety. Soya PL mixtures with human brain PT demonstrating high procoagulant activity are characterized by 0.3-0.8 ratio of total acid to total neutral PL and acid number 40-60 mg KOH. Isolated from soya PL procoagulants accelerate clotting of plasma from healthy subjects 2.2-fold being just a little less active than brain PL. This makes the above procoagulants usable as test-reagent for evaluation of hemostasis.