RESUMO
Thyroid carcinoma (THCA) is a malignant tumor of the endocrine system. Previous studies have revealed the vital roles of microRNAs (miRNAs/miRs) in THCA procession. The present study aimed to explore the effects of miR15b5p on the progression of THCA and its targeting mechanism. The data of THCA and healthy samples were firstly collected from starbase2.0 and used to analyze the relationship of miR15b5p with THCA. Dualluciferase assay was performed to detect the direct interaction between miR15b5p and the predicted target gene GDP dissociation inhibitor 2 (GDI2). The effects of miR15b5p and GDI2 on the overall survival of patients with THCA were analyzed using KaplanMeier analysis with log rank test. Cell Counting Kit8 and Transwell assays were conducted to assess the impacts of miR15b5p and GDI2 on the proliferation and invasion of THCA cells. Reverse transcriptionquantitative PCR and western blot analyses were performed to analyze the expression levels of the related miRNAs and proteins, respectively. miR15b5p was found to be downregulated both in THCA tissues and cells, and the low expression of miR15b5p was associated with the short overall survival time of patients. Moreover, the upregulation or downregulation of miR15b5p could inhibit or enhance the proliferation and invasion of THCA cells, respectively. miR15b5p reduced the protein expression levels of matrix metalloproteinase (MMP)2 and MMP9, which were related to cell invasion. Furthermore, GDI2, which was enhanced in THCA and related to the poor prognosis of patients with THCA, was identified as the target gene of miR15b5p and negatively regulated by miR15b5p. Additional experiments demonstrated that GDI2 overexpression could significantly reduce the antitumor effect of miR15b5p and its inhibitory action on the expression levels of MMP2 and MMP9. Thus, the results indicated a potential tumor suppressive role of miR15b5p in THCA, which was mainly exerted by targeting GDI2 and modulating MMP2 and MMP9. These findings will increase the understanding on the pathogenesis of THCA and provide novel candidates for THCA therapy.
Assuntos
Regulação para Baixo , Inibidores de Dissociação do Nucleotídeo Guanina/genética , MicroRNAs/genética , Neoplasias da Glândula Tireoide/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Linhagem Celular Tumoral , Proliferação de Células , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Análise de Sobrevida , Neoplasias da Glândula Tireoide/mortalidade , Adulto JovemRESUMO
Laryngeal squamous cell carcinoma (LSCC) is the most common type of laryngeal cancer with poor prognosis. In the present study, we aimed to investigate the biological role of long non-coding RNA OIP5-AS1 in LSCC. The results demonstrated that the expression levels of OIP5-AS1 were significantly increased in LSCC tissues and cell lines. High expression of OIP5-AS1 was closely correlated with lymph node metastasis and advanced clinical stage of LSCC patients. Moreover, in vitro assays showed that OIP5-AS1 overexpression promoted the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of LSCC cells, whereas OIP5-AS1 knockdown exerted suppressive effects on LSCC cells. Furthermore, OIP5-AS1 was confirmed to serve as a competing endogenous RNA of miR-204-5p in LSCC cells, and restoration of miR-204-5p counteracted the OIP5-AS1-mediated oncogenic effects. In conclusion, our study provides promising evidence that lncRNA OIP5-AS1 functions as a tumor promoter in LSCC and may be used as a potential target for LSCC therapy.