RESUMO
Objective: To evaluate the prediction power of HIV infection risk assessment tool and the applicability in MSM in Guizhou province. Methods: MSM were recruited through snowball sampling method. Questionnaire surveys were conducted among the MSM using HIV infection risk assessment tool, and combined with HIV serologic test results, the risk prediction power of HIV infection risk assessment tool was evaluated. Results: A total of 3 379 MSM were recruited from January 2018 to December 2019 in Guizhou. The HIV infection rate was 3.3%(111/3 379). The mean risk scores of HIV positive and HIV negative MSM were (12.15±3.08) and (12.07±3.07), respectively. The difference in risk score was significant between MSM with different HIV status (t=8.69, P<0.001). According to the principle of decision tree, individual risk scores were divided into following three categories: ≤11.96, 11.97-14.80 and >14.80, the HIV infection rate was 0.8%, 4.3% and 8.6% respectively, suggesting that the higher the individual risk score was, the higher the HIV infection rate was (trend χ2=88.18, P<0.001). Multivariate logistic regression analysis showed that the higher the individual risk score was, the higher the risk of HIV infection was. Compared to the total score ≤11.96, the aOR values at total scores of 11.97-14.80 and >14.80 were 6.34 (95%CI: 3.38-11.88) and 14.07(95%CI: 7.44-26.61), respectively. The risk of HIV infection in Miao ethnic group was higher than that in Han ethnic group (aOR=1.83, 95%CI:1.04-3.21), and the risk of HIV infection in those with education level of primary school and below was higher than that in undergraduates or those with education level of junior college and above (aOR=2.50, 95%CI:1.06-5.88), and the risk of HIV infection was higher in those who had bisexual behaviors than in those who had homosexual behaviors (aOR=1.95, 95%CI:1.19-3.19). The risk of HIV infection was higher in those who had never received HIV testing (aOR=1.53, 95%CI:1.01-2.33). The area under the receiver operating characteristic (ROC) curve and area under ROC (AUC) for HIV infection prediction was 0.751 (95%CI:0.710-0.792, P<0.001). The maximum Youden's index was individual risk score of 12.56, and the sensitivity of the risk assessment tool was 0.838, and its specificity was 0.412. Conclusions: The results of HIV infection risk assessment tool in Guizhou indicated that in MSM the higher the individual risk score, the higher the risk of HIV infection is. The tool can be used to evaluate the risk of HIV infection in MSM, but the specificity should be improved.
Assuntos
Infecções por HIV , Minorias Sexuais e de Gênero , Bissexualidade , Infecções por HIV/epidemiologia , Homossexualidade Masculina , Humanos , Masculino , Medição de RiscoRESUMO
OBJECTIVE: Recent studies have provided evidence that long noncoding RNA SNHG7 is highly expressed and associated with poor clinical outcomes in cancer patients. The meta-analysis is aimed to evaluate the prognostic value of SNHG7 across various cancers. MATERIALS AND METHODS: Eligible studies about prognosis and clinicopathological features of SNHG7 expression in all kinds of tumors were collected by searching the databases of PubMed, Web of Science, Embase, Cochrane Library from inception through August 13, 2020. Odds ratios (ORs) and hazard ratios (HRs) with 95% confidence intervals (CIs) from eligible studies were extracted and pooled to investigate the association between SNHG7 and survival or clinicopathology by STATA 16.0 software. RESULTS: A total of 13 studies enrolling 1029 cancer patients met the inclusion criteria in this meta-analysis. Based on the results, over-expressed SNHG7 was associated with deeper tumor invasion (OR: 2.76; 95% CI: 1.98-3.86; p: 0.000), earlier lymphatic metastasis (OR: 4.22; 95% CI: 3.04-5.86; p: 0.000), more advanced tumor stage (OR: 3.49; 95% CI: 2.45-4.98; p: 0.000) and poor histologic grade (OR: 2.23; 95% CI: 1.33-3.74; p: 0.002), but not with sex, age, tumor size and distant metastasis. As for prognosis, patients with high expression of SNHG7 were more likely to have shorter overall survival (OS) (HR: 1.64; 95% CI: 1.38-1.94; p: 0.000) and disease-free survival (DFS) (HR: 1.37; 95% CI: 1.09-1.71; p: 0.006). CONCLUSIONS: SNHG7 may serve as a novel biomarker in terms of predicting prognosis and clinicopathological characters in various human cancers.
Assuntos
Neoplasias/genética , RNA Longo não Codificante/genética , Humanos , Neoplasias/diagnóstico , SoftwareRESUMO
Both interleukin (IL)-33 and IL-25 induce Th2-type cytokine production by various cell types, suggesting that they may contribute to development of allergic disorders, however, the immunomodulatory effects of IL-33 and IL-25 in ovalbumin (OVA)-induced allergic rhinitis (AR) remain unclear. In the present study, anti-IL-33 and anti-IL-25 Abs were administrated intranasally during rechallenge in OVA-induced AR. Immunomodulatory effects were evaluated by measuring nasal rubbing, sneezing occurrence, serum OVA-specific antibodies, Th2 immune responses, neutrophil, eosinophil and mast cell recruitment into the nasal mucosa. We found that treatment with anti-IL-33 Ab markedly reduced nasal rubbing, sneezing events, Th2 immune responses, serum OVA-specific IgE and IgG1 levels, mucosal neutrophil, eosinophil and mast cell infiltration. In contrast, the effect of IL-25 antagonism was limited to attenuating the Th2 immune responses, and neutrophil and eosinophil infiltration. These observations indicate that IL-33 and IL-25 play a pathogenic role in an established AR mouse model, with a greater contribution of IL-33 than IL-25. Our findings suggest that IL-33 neutralization may be a potential approach for treatment of AR.
Assuntos
Interleucina-17 , Rinite Alérgica , Animais , Citocinas , Modelos Animais de Doenças , Imunidade , Interleucina-33 , Camundongos , Camundongos Endogâmicos BALB C , Mucosa Nasal , Ovalbumina , Rinite Alérgica/induzido quimicamente , Rinite Alérgica/tratamento farmacológico , Células Th2RESUMO
AIM: To investigate the innervation pattern of extra-ocular muscles in patients with clinically diagnosed Duane's ocular retraction syndrome (DRS) using magnetic resonance imaging (MRI). MATERIALS AND METHODS: The study population consisted of 11 patients. Six patients had type I DRS (eight eyes), four patients had type II DRS (five eyes) and one patient had inverse DRS. Images were acquired using a Siemens 3 T MRI system. The type of DRS, corresponding innervation findings, and condition of the affected muscles were evaluated by two experienced neuroradiologists in consensus. RESULTS: All patients with clinically diagnosed DRS type I showed absence of the abducens nerve (CN6), hypoplasia of the superior oblique muscle (SOM), and aberrant innervation of lateral rectus muscle (LRM) by an extra branch of oculomotor nerve (CN3). All patients with type II DRS show dual-innervation of the LRM (by CN6 and an aberrant CN3 branch) and hypoplasia of SOM. The single patient with inverse DRS showed hypoplasia of CN3, the medial rectus muscle (MRM), the inferior rectus muscle (IRM), and the inferior oblique muscle (IOM). CONCLUSION: Each type of DRS has characteristic MRI appearances. Therefore, MRI is a useful diagnostic tool for the confirmation and classification of suspected cases of DRS.
Assuntos
Nervo Abducente/patologia , Síndrome da Retração Ocular/patologia , Imageamento por Ressonância Magnética , Músculos Oculomotores/patologia , Nervo Oculomotor/patologia , Nervo Abducente/anormalidades , Adulto , Criança , Pré-Escolar , China/epidemiologia , Diagnóstico Diferencial , Síndrome da Retração Ocular/genética , Síndrome da Retração Ocular/fisiopatologia , Feminino , Humanos , Masculino , Músculos Oculomotores/inervação , Nervo Oculomotor/fisiopatologiaRESUMO
Many different classes of drugs induce fetal hemoglobin (HbF) including histone deacetylase (HDAC) inhibitors such as butyrate and trichostatin A. Although these agents induce gamma-globin expression in culture many are ineffective in vivo, therefore research efforts continue to identify clinically useful fetal globin inducers. We and others demonstrated a role for p38 mitogen activated protein kinase (MAPK) in gamma-globin promoter activation by HDAC inhibitors. In this study we determined the ability of scriptaid, a novel HDAC inhibitor, to induce gamma-globin expression via p38 MAPK signaling. Scriptaid induced gamma-globin in K562 cells and human erythroid progenitors. Furthermore the p38-selective inhibitor SB203580 completely reversed the ability of scriptaid to induce HbF. To test the potential efficacy of scriptaid in humans, in vivo studies were completed in beta-YAC transgenic mice where the gamma-gene is completely silenced. Scriptaid induced reticulocytosis and human gamma-globin mRNA synthesis. At a concentration of 1 mg/kg/day given by intraperitoneal injections twice weekly we observed a significant 1.8-fold increase in gamma-globin mRNA transcripts. The potential for scriptaid as a treatment option for sickle cell disease will be discussed.
Assuntos
Células Precursoras Eritroides/efeitos dos fármacos , Hemoglobina Fetal/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Globinas/genética , Inibidores de Histona Desacetilases , Hidroxilaminas/farmacologia , Quinolinas/farmacologia , Anemia Falciforme/tratamento farmacológico , Animais , Butiratos/farmacologia , Inibidores Enzimáticos/farmacologia , Células Precursoras Eritroides/química , Células Precursoras Eritroides/metabolismo , Hemoglobina Fetal/biossíntese , Inativação Gênica , Globinas/biossíntese , Humanos , Ácidos Hidroxâmicos/farmacologia , Hidroxilaminas/uso terapêutico , Imidazóis/farmacologia , Células K562 , Camundongos , Camundongos Transgênicos , Piridinas/farmacologia , Quinolinas/uso terapêutico , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Transdução de Sinais/efeitos dos fármacos , Proteínas Quinases p38 Ativadas por Mitógeno/fisiologiaRESUMO
HBx, a transcriptional transactivating protein of hepatitis B virus (HBV), is required for viral infection and has been implicated in virus-mediated liver oncogenesis. However, the molecular mechanism for its influence on cell remains largely unknown. It was proved that HBx need the help of host cell proteins to exert its function by binding to them. During purifying of GSTX (fusion protein of GST and HBx) expressed in E. coli, we found that it can bind specifically with GrpE (HSP60) and DnaK (HSP70) of E. coli while GST cannot. Using GST pull-down, two-dimensional gel electrophoresis and mass spectrum, we found that GSTX can also bind to human mitochondrial HSP60 and HSP70, which are homologues of GrpE and DnaK. These interactions between HBx and mitochondrial HSP60 and HSP70 are supported by the result of co-immunoprecipitation experiment. It means that HBx can form complex with E. coli and human HSP60 and HSP70. The implication of HBx, HSP60 and HSP70 complex in molecular mechanism of virus infection is discussed.
Assuntos
Chaperonina 60/metabolismo , Proteínas de Choque Térmico HSP70/metabolismo , Antígenos da Hepatite B/metabolismo , Mitocôndrias/metabolismo , Transativadores/metabolismo , Sequência de Aminoácidos , Animais , Células COS , Linhagem Celular , Chaperonina 60/genética , Chlorocebus aethiops , Escherichia coli/metabolismo , Proteínas de Choque Térmico HSP70/genética , Humanos , Dados de Sequência Molecular , Transportadores de Ânions Orgânicos/metabolismo , Ligação Proteica , Alinhamento de Sequência , Transativadores/genética , Proteínas Virais Reguladoras e AcessóriasRESUMO
Parvovirus B19 involvement was investigated in 30 children with severe aplastic anaemia. Active or recent parvovirus B19 infection, as shown by B19 DNA viraemia, positive B19 specific IgM antibodies, or both, was diagnosed in six patients. There were no other plausible causes. We suggest that parvovirus B19 infection might be associated with severe aplastic anaemia.
Assuntos
Anemia Aplástica/virologia , Infecções por Parvoviridae/complicações , Parvovirus B19 Humano , Adolescente , Criança , Pré-Escolar , Feminino , Humanos , Lactente , MasculinoRESUMO
In this paper, the iodine beta-cyclodextrin complexes prepared in different techniques were investigated by Fourier transformation infrared spectrum(FTIR), FT-Raman, Ultra-violet absorption spectrum in qualitative and quantitative analysis. Results showed that the iodine existed in dissociated, bundle and polymerized forms in the complexes. Contras to the method of alcohol craft, the sample prepared in water craft method included a higher capacity of iodine, but it was not stable enough to keep a constant high iodine concentration. This analysis method was simple and convenient, it is useful for quality control of the iodine beta-cyclodextrin complexes raw material medicine.
Assuntos
Quelantes/química , Ciclodextrinas/química , Iodo/química , Anti-Infecciosos/química , Estabilidade de Medicamentos , Espectrofotometria Ultravioleta , Espectroscopia de Infravermelho com Transformada de Fourier , Análise Espectral RamanRESUMO
Affinity capillary electrophoresis (ACE) has been used to investigate the epitope on the human immunodeficiency virus (HIV) core protein p24 recognized by the monoclonal antibody (mAb) 13-102-100. The affinity of a series of peptides with N- and C-terminal truncations of the epitope sequence determined by mass spectrometry was studied. The peak area change assay was used for the study of the interactions of the mAb with those peptides, exhibiting tight binding to the mAb, and the migration time shift assay was used to probe the relative affinities of peptides showing weak binding to the mAb. The experimental results show that the monoclonal antibody 13-102-100 recognizes the peptide VHPVHAGPIAP with highest affinity. Smaller peptides incorporating only part of the epitope, however, are recognized to some extent in the ACE experiments.
Assuntos
Eletroforese Capilar/métodos , Epitopos/química , Proteína do Núcleo p24 do HIV/química , Proteína do Núcleo p24 do HIV/imunologia , Sequência de Aminoácidos , Anticorpos Monoclonais , Reações Antígeno-Anticorpo , Anticorpos Anti-HIV , Humanos , Cinética , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/imunologia , Ligação ProteicaRESUMO
Postpartal determination of lactate and glucose in the umbilical cord whole blood of 139 successive deliveries utilizing biosensors (blood gas analysator 865, Ciba Corning) are presented. The median lactate value in the umbilical arterial blood is 4.45 mmol/l and in the venous blood 4.23 mmol/l. Following categorization into control and high-risk groups, the arterial mean values are 4.23 mmol/l and 6.39 mmol/l and the respective venous values are 3.95 mmol/l and 5.04 mmol/l. Using the U-test these differences between the control and high-risk groups are significant. The mean of the measured lactate correlates significantly with the mean of the calculated base excess (< 0.001). The mean glucose value in the umbilical arterial blood is 78 mg/dl and in the venous 93 mg/dl. Between high-risk and control group no significant difference is found.
Assuntos
Técnicas Biossensoriais , Gasometria/instrumentação , Glicemia/análise , Sangue Fetal/química , Ácido Láctico/sangue , Asfixia Neonatal/sangue , Asfixia Neonatal/diagnóstico , Desenho de Equipamento , Feminino , Humanos , Recém-Nascido , Masculino , Gravidez , Valores de Referência , Fatores de RiscoRESUMO
BACKGROUND: Thrombospondin-1 (TSP-1) is a matrix-bound adhesive glycoprotein. Breast carcinoma cells exhibit increased expression of a novel TSP-1 receptor. We evaluated the role of this receptor in breast cancer adhesion and progression. METHODS: Adhesion assays were performed to evaluate MDA-MB-231 breast cancer cell adhesion to TSP-1 in vitro in the presence of either nonimmune immunoglobulin G(IgG) or anti-TSP-1 receptor IgG. Receptor-mediated tumor cell progression was evaluated in athymic nude mice. Mice were inoculated with MDA-MB-231 breast cancer cells and randomized to treatment with intraperitoneal injections of saline solution, nonspecific IgG antibody, or an anti-TSP-1 receptor antibody every other day for 20 days. Mice were killed at 21 days. The peritoneal cavity was examined grossly for primary tumor implantation. The liver and lungs were examined histologically for micrometastases. RESULTS: MDA-MB-231 breast cancer cells adhered to TSP-1 in vitro. This adhesion was inhibited to 10% of control by anti-TSP-1 receptor antibody (p < 0.005). Anti-TSP-1 receptor antibody inhibited in vivo breast cancer progression. Mice treated with control IgG antibody or saline solution alone exhibited extensive intraperitoneal seeding. Only one mouse treated with the anti-TSP-1 receptor antibody exhibited any intraperitoneal tumor seeding (p < 0.01). CONCLUSIONS: These data suggest that TSP-1 and its receptor play an important role in breast cancer progression.
Assuntos
Neoplasias da Mama/patologia , Antígenos CD36/imunologia , Animais , Especificidade de Anticorpos , Moléculas de Adesão Celular/fisiologia , Transformação Celular Neoplásica/imunologia , Modelos Animais de Doenças , Feminino , Humanos , Camundongos , Camundongos Nus , Transplante de Neoplasias , Neoplasias Peritoneais/secundário , Coelhos , Células Tumorais Cultivadas/química , Células Tumorais Cultivadas/citologia , Células Tumorais Cultivadas/fisiologiaRESUMO
BACKGROUND: Thrombospondin (TSP), a cell matrix protein, and transforming growth factor beta (TGF-beta), a growth regulatory protein, play roles in tumor progression. The purpose of this study was to investigate the effects of TSP and TGF-beta on tumor cell invasion. MATERIALS AND METHODS: Tumor cell invasion assays were performed using a modified Boyden chamber apparatus with collagen-coated membranes. The KB oral carcinoma cell line was studied in serum-free media. Invasion was measured as the summation of the number of cells in five representative low-power fields (x 100) traversing the collagen barrier after a 3-hour incubation period. The effects of antibodies against TSP, TGF-beta and the cysteine-serine-valine-threonine-cysteine-glycine (CSVTCG)-specific TSP receptor were also evaluated. RESULTS: TSP caused a dose-dependent stimulation of tumor cell invasion. Antibodies against TSP, its CSVTCG-specific receptor, and TGF-beta inhibited TSP-promoted invasion by 50% to 71%. CONCLUSIONS: TSP and its CSVTCG-specific receptor promote KB cell invasion of collagen through the production and/or activation of TGF-beta.
Assuntos
Carcinoma de Células Escamosas/patologia , Moléculas de Adesão Celular/farmacologia , Colágeno/efeitos dos fármacos , Glicoproteínas de Membrana/farmacologia , Neoplasias Bucais/patologia , Sequência de Aminoácidos , Anticorpos Monoclonais , Antígenos CD36/efeitos dos fármacos , Antígenos CD36/imunologia , Antígenos CD36/fisiologia , Carcinoma de Células Escamosas/metabolismo , Moléculas de Adesão Celular/administração & dosagem , Moléculas de Adesão Celular/imunologia , Contagem de Células , Colágeno/metabolismo , Meios de Cultura Livres de Soro , Cultura em Câmaras de Difusão , Progressão da Doença , Relação Dose-Resposta a Droga , Humanos , Imunoglobulina G , Integrinas/efeitos dos fármacos , Integrinas/imunologia , Integrinas/fisiologia , Glicoproteínas de Membrana/administração & dosagem , Glicoproteínas de Membrana/imunologia , Membranas Artificiais , Microscopia de Contraste de Fase , Neoplasias Bucais/metabolismo , Invasividade Neoplásica , Cimento de Policarboxilato , Trombospondinas , Fator de Crescimento Transformador beta/imunologia , Fator de Crescimento Transformador beta/farmacologia , Células Tumorais CultivadasRESUMO
The FLP recombinase promotes a site-specific recombination reaction in the 2mu plasmid of yeast. The protein-DNA complex that carries out the reaction is asymmetric. Three FLP monomers bound to specific FLP-recognition sequences are required to efficiently carry out one set of reciprocal DNA cleavage and strand exchange events on a Holliday junction substrate. If a fourth monomer plays an auxiliary role in the reaction, it is bound without sequence specificity. The data suggest a modified model for cleavage of DNA in trans by the FLP recombinase that might help reconcile some seemingly conflicting resulted obtained with integrase class recombinases.
Assuntos
DNA Nucleotidiltransferases/metabolismo , Plasmídeos , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Sequência de Bases , Sítios de Ligação , DNA Nucleotidiltransferases/química , Proteínas Fúngicas/metabolismo , Cinética , Substâncias Macromoleculares , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Conformação Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Recombinação Genética , Especificidade por SubstratoRESUMO
The bcr/abl fusion gene in 20 patients with chronic myeloid leukemia (CML) was detected by RNA polymerase chain reaction, which used mRNA as the starting material to generate cDNA with reverse transcriptase followed by PCR amplification (RNA/PCR). Amplification of a sequence spanning the bcr/abl junction region was achieved by using peripheral blood cells as the source of mRNA from all 20 patients with CML, including 3 cases of Ph (-) CML, and cell line K562 was derived from patients with CML. No amplification was seen when mononuclear cells from 3 normal individuals, 2 patients with lymphoma and cell line HL-60 were used. The presence or absence of bcr exon 3 in the fusion mRNA was determined by the size of the amplified fragments. Of the 20 CML patients, 15 showed only the 165-bp amplified band (indicating retention of bcr exon 3), one showed only the 90-bp amplified band, and 4 showed both 165-bp and 90-bp bands. Both bands were seen more frequently in blast crisis than in remission and chronic phase.
Assuntos
Proteínas de Fusão bcr-abl/genética , Genes abl/genética , Leucemia Mielogênica Crônica BCR-ABL Positiva/genética , RNA Neoplásico/genética , Adolescente , Adulto , Sequência de Bases , Criança , Cromossomos Humanos Par 22 , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Cromossomo Filadélfia , Reação em Cadeia da Polimerase , Translocação GenéticaRESUMO
Maternal venous blood (MB), umbilical blood (UB) and placental tissue (PT) were collected from 40 HBsAg positive mothers and their neonates, and also blood from 17 babies aged 3-6 months old of this group (BB). All samples were determined for hepatitis B virus (HBV) DNA by molecular hybridization technique using Bio-HBV DNA probe. The results showed: HBV DNA positive rate of MB was 35.0%, 47.5% for UB, 75.0% for PT and 29.4% for BB. 32 P-HBV DNA probe was also used to examine MB and PT. The positive rates of HBV DNA were 30.0% and 70.0% respectively. There was no significant difference between the results of the 2 probes. We considered: (1) With the rapid development of HBV detection technique, the detectable rate of intrauterine infection increases accordingly. (2) Besides transplacental infection, other transmission routes might be existed. (3) The detection of HBV DNA in UB, PT and in the blood of babies born by HBV DNA positive mother within 6 months old provides the reliable diagnosis. (4) HBV DNA molecular hybridization is an accurate and sensitive method for the diagnosis of HBV intrauterine infection.
Assuntos
DNA Viral/análise , Vírus da Hepatite B/isolamento & purificação , Hepatite B/transmissão , Complicações Infecciosas na Gravidez/microbiologia , Vilosidades Coriônicas/microbiologia , Sondas de DNA , Feminino , Sangue Fetal/microbiologia , Antígenos de Superfície da Hepatite B/sangue , Humanos , Recém-Nascido , Hibridização de Ácido Nucleico , GravidezRESUMO
The FLP recombination target (FRT) can be cut in half so that only one FLP protein binding site is present (a "half site"). FLP protein binds the half sites and joins them into dimeric, asymmetric head-to-head complexes held together chiefly by strong noncovalent interactions. These complexes react with full (normal) FRT sites to generate a variety of products. Analysis of these DNA species reveals that the reaction follows a well-defined reaction pathway that generally parallels the normal reaction pathway. The system is useful in analyzing early steps in recombination, since the identity of the products in a given recombination event unambiguously pinpoints the order in which the cleavage and strand exchange reactions occur. Two conclusions are derived from the present study: (i) Formation of the dimeric head-to-head complex of half sites is a prerequisite to further steps in recombination. (ii) The identity of the base pairs at positions 6 and -6 within the FRT site has a subtle effect in directing the first strand exchange event in the reaction to predominantly one of two possible cleavage sites. In addition, results are presented that suggest that a DNA-DNA pairing intermediate involving only two base pairs of the core sequence is formed prior to the first cleavage and strand exchange. DNA-DNA interactions may therefore not be limited to the isomerization step that follows the first strand exchange.
Assuntos
DNA Nucleotidiltransferases/metabolismo , Recombinação Genética , Sequência de Bases , DNA/genética , DNA/metabolismo , DNA/ultraestrutura , DNA Nucleotidiltransferases/genética , Microscopia Eletrônica , Modelos Genéticos , Dados de Sequência Molecular , Plasmídeos , Especificidade por SubstratoRESUMO
75 patients with hypertension syndrome of pregnancy (HSP) were randomly designed to 2 groups: the control group treated with magnesium sulfate (20-25g/d) and the Ligustrazine (120-160mg/d) group. The results of Ligustrazine group compared with the control group were as follows: (1) Mean arterial pressure was significantly decreased (P less than 0.01). (2) Edema and proteinuria was lowered (P less than 0.05). (3) The condition of rheology was improved, especially, hematocrit was significantly decreased (P less than 0.001). (4) The positive rate of NST and Apgar's score were not different between the 2 groups. Clinical monitoring showed Ligustrazine without side effects in the group. Mechanisms of Ligustrazine in HSP were (1) dilating blood vessel; (2) improving kidney function; (3) improving microcirculatory and rheology.
Assuntos
Inibidores da Agregação Plaquetária/uso terapêutico , Pré-Eclâmpsia/tratamento farmacológico , Pirazinas/uso terapêutico , Vasodilatadores/uso terapêutico , Adulto , Avaliação de Medicamentos , Feminino , Humanos , Sulfato de Magnésio/uso terapêutico , GravidezRESUMO
By means of DEAE-Sepharose CL-6B ion exchange chromatography and TSK-GEL G2000 SW high-performance gel filtration, a purified protein with fibrinolytic activity was obtained from the venom of Agkistrodon halys brevicaudus (Korean mamushi). The protein was homogeneous as judged by isoelectric focusing electrophoresis and high-performance gel filtration. Its mol. wt is 39,200 and its isoelectric point 4.12. The specific fibrinolytic activity of the protein was 3.2 times higher than that of the crude venom. The fibrinolytic activity of the purified principle was 33 units/mg protein (units of standard urokinase activity).
Assuntos
Venenos de Crotalídeos/enzimologia , Fibrinolíticos/farmacologia , Aminoácidos/análise , Fenômenos Químicos , Físico-Química , Cromatografia DEAE-Celulose , Cromatografia em Gel , Cromatografia por Troca Iônica , Venenos de Crotalídeos/química , Venenos de Crotalídeos/farmacologia , Focalização Isoelétrica , Peso Molecular , Ativador de Plasminogênio Tipo Uroquinase/análiseRESUMO
When the FLP recombination target (FRT) is cut in half so that only one FLP protein-binding site is present, FLP protein forms a complex in which two such sites are linked head to head. Although held together exclusively by noncovalent interactions, this complex survives electrophoresis in an agarose gel and exhibits a half-life that can be measured in hours. Characterization of this complex indicates that a very stable, asymmetric dimeric complex of FLP protein monomers bound to the FRT is a likely early intermediate in FLP-mediated site-specific recombination. The apparent asymmetry is a property of the protein components of the complex. Even though the DNA components form a perfect palindrome, only one of the two possible DNA cleavage steps takes place in the course of complex formation. Formation of this complex does not occur with half-FRT site DNA substrates that preclude head to head monomer contact or when a FLP mutant protein is used that binds the FRT site but cannot cleave it. Trimeric and tetrameric complexes are also observed, the latter at very low frequency. These results are discussed in terms of an expanded model for early events in FLP-mediated site-specific recombination.