RESUMO
Objective To optimize the color conditions of spectrophotometric determination of total saponins in Fructus Trichosanthis. Methods Several factors affecting the content determination of total saponins in Fructus Trichosanthis were studied , including the color temperature,color time,5%vanillin-acetic acid dosage,as well as the amount of perchloric acid. The analysis con-ditions were optimized by Box-Behnken response surface method. Results The optimized conditions were:temperature 60℃,the col-or time 25 min,5%vanillin-acetic acid 0.1 ml and perchloric 0.6 ml. The color was stable and the determination results were accurate. Conclusion The color conditions determined by Box-Behnken response surface method can be used for content determination of total saponins in Fructus Trichosanthis.
RESUMO
Objective To study the similarity of chemical fingerprints of different proportion of Gualoupi-Gualouzi and Fruc?tus Trichosanthis,so as to provide the theoretical basis for the reasonable proportion of Gualoupi-Gualouzi. Methods The HPLC fin?gerprints and the common patterns of 6 the proportion of the 6 kinds of compatibility(30:70,35:65,40:60,45:55,50:50 and 55:45)of Gualoupi-Gualouzi and Fructus Trichosanthis were established,and the similarity of chemical fingerprints of different compati?bility proportion of Gualoupi-Gualouzi and Fructus Trichosanthis between their common patterns were compared. Results Compared with the common pattern of Fructus Trichosanthis,the similarities of the common patterns of chemical fingerprints of the above 6 kinds of compatibility proportion of Gualoupi-Gualouzi were 0.8808 ± 0.0407,0.8930 ± 0.0236,0.8995 ± 0.0195,0.9033 ± 0.0190,0.9363 ± 0.0082 and 0.8904 ± 0.0144,respectively. Among them,Gualoupi-Gualouzi ratio of 50:50 had the highest similarity,and compared with other groups,the differences were statistically significant(P<0.05 or 0.01). Conclusion The optimum proportion of Gualoupi-Gualouzi can be determined by the fingerprint technology,which provides the theoretical basis for the reasonable proportion of Gualou?pi-Gualouzi.