RESUMO
Ce-MOF was synthesized by a solvothermal synthesis method and was used to simultaneously remove phosphate, fluoride and arsenic (V) from water by adsorption. Ce-MOF was characterized by a nitrogen adsorption-desorption isotherm, scanning electron microscopy, and infrared spectroscopy. The effects of initial concentration, adsorption time, adsorption temperature, pH value and adsorbent on the adsorption properties were investigated. A Langmuir isotherm model was used to fit the adsorption data, and the adsorption capacity of phosphate, fluoride, and arsenic (V) was calculated to be 41.2 mg·g-1, 101.8 mg·g-1 and 33.3 mg·g-1, respectively. Compared with the existing commercially available CeO2 and other MOFs, Ce-MOF has a much higher adsorption capacity. Furthermore, after two reuses, the performance of the adsorbent was almost unchanged, indicating it is a stable adsorbent and has good application potential in the field of wastewater treatment.
RESUMO
A novel dual-mode nanoprobe (Apt@MNPS) was created for the detection of autophagy-related miRNAs to monitor the autophagic level and study the effect of PTT on autophagy. Interestingly, using our developed probe, PTT was found to be able to activate the autophagy by down regulation of miR-18a* and miR-4802, which in turn restricted the PTT efficiency for cancer.
Assuntos
Autofagia/efeitos dos fármacos , Autofagia/efeitos da radiação , MicroRNAs/análise , Nanosferas/química , Aptâmeros de Nucleotídeos/química , Aptâmeros de Nucleotídeos/genética , Aptâmeros de Nucleotídeos/farmacologia , Carbocianinas/química , Regulação para Baixo , Corantes Fluorescentes/química , Humanos , Hipertermia Induzida , Limite de Detecção , Células MCF-7 , MicroRNAs/genética , MicroRNAs/metabolismo , Molibdênio/química , Molibdênio/farmacologia , Molibdênio/efeitos da radiação , Nanosferas/efeitos da radiação , Neoplasias/terapia , Hibridização de Ácido Nucleico , Óxidos/química , Óxidos/farmacologia , Óxidos/efeitos da radiação , Tamanho da Partícula , Fototerapia/métodos , Espectrometria de Fluorescência/métodos , Análise Espectral Raman/métodosRESUMO
A novel capillary electrophoresis with laser-induced fluorescence detection method has been developed for the analysis of aldehyde metabolism biomarkers for oxidative stress in exhaled breath condensate (EBC), and fluorescein 5-thiosemicarbazide was used as a derivatization reagent. In a simple capillary zone electrophoresis mode, ten low molecular weight aldehydes (LMWAs) could be well separated within 30 min. The reaction efficiency was doubled by increasing sample solution pH and magnetic stirring, and the LODs of this method reached 0.16-3.4 nM (S/N = 3). Acceptable recoveries (82.1-115%) were obtained for EBC samples, and the RSD data were within 7.9%. This developed method has been applied for the analyses of EBC samples and evaluation of the correlation between smoking and the contents of aldehyde metabolites in EBC. Due to no need of buffer additives and sample preconcentration, this proposed method may provide an appealing alternative for the trace analyses of LMWAs in noninvasive biofluids. Graphical abstract á .
